Novel Multimodal Selectors for CE and HPLC

描述(申请人提供)：目前，高效液相色谱分离发展最快的领域是生物分子分离和液质联用(LC-MS)。尽管在过去的几十年里，质谱学取得了惊人的进步，但与质谱计相结合的分离所固有的缺陷仍然对获取生物样品中的全部信息内容构成了相当大的障碍。生物分子分离具有挑战性，因为它们呈现了从疏水到极性再到静电的各种相互作用模式。拟议的工作解决了迫切需要改进的技术，用于生物聚合物(例如多肽、蛋白质、寡核苷酸)的新颖和更通用的分离战略，同时能够表征溶质/离子液体相互作用。这项拟议工作的目标是将新兴的离子液体领域整合到生物分子分离的新分离策略中，目的是提供一种快速评估离子液体/目标分析物相互作用的方法，这是一种能够分离复杂生物分子混合物的新型固定相。 我们假设所提出的选择器将为分离多种生物分子分析物提供多模式保留。 两个具体目标将解决这一假设： 1.离子液体选择剂的电致化学研究毛细管电泳法将用于快速筛选离子液体/选定分析物的相互作用。我们将把迁移数据与离子液体/分析物亲和力联系起来。 2.离子液体选择剂的LC研究随着这种新介质所包含的分离空间的规划，将从系统地从小分子(例如，芳烃、核苷酸、氨基酸)开始，扩展到较大的分子(例如，肽、多核苷酸)，从而获得关于建议的高效液相色谱吸附剂的保留数据。

DESCRIPTION (provided by applicant): Currently, the fastest growing areas of high performance liquid chromatographic separations are in the areas of biomolecule separations and liquid chromatography-mass spectrometry (LC-MS). While the last few decades have seen phenomenal progress in mass spectrometry, the shortfalls inherent in the separations coupled to the mass spectrometer still present considerable barriers to accessing the full information content in biological samples. Biomolecule separations are challenging because they present a variety of interaction modalities ranging from hydrophobic to polar to electrostatic. The proposed work addresses the urgent need for improved technologies for novel and more universal separation strategies for biopolymers (e.g., polypeptides, proteins, oligonucleotides) while simultaneously enabling characterization of solute/ionic liquid interactions. The objective of the proposed work is to integrate the emerging field of ionic liquids into novel separation strategies for biomolecular separations with the goal of providing a rapid method for assessing ionic liquids/target analyte interactions a new class of stationary phases enabling separations of complex biomolecular mixtures. We hypothesize that the proposed selectors will provide multimodal retention for the separation of a wide variety of biomolecular analytes. Two specific aims will address this hypothesis: 1. CE Studies of Ionic Liquid Selectors. Capillary electrophoresis will be used to rapidly screen for ionic liquids/selected analyte interactions. We will relate migration data to ionic liquid/analyte affinity. 2. LC Studies of Ionic Liquid Selectors. Retention data will be obtained on the proposed HPLC sorbents beginning systematically with small molecules (e.g., aromatics, nucleotides, amino acids) and extending to larger molecules (e.g., peptides, polynucleotides) as the separation space encompassed by this new media is mapped out.