Molecular Analysis of Juvenile Hormone Action

保幼激素作用的分子分析

• 批准号: 7172999
• 负责人: SUBBA R PALLI
• 金额: $ 19.34万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7172999
• 关键词: Molecular; Analysis; Juvenile; Hormone; Action

DESCRIPTION (provided by applicant): Some insects compete with us for food and transmit human and livestock diseases, but other insects are beneficial. Therefore, there has been a continuous demand for the development of insect control methods that are target-specific. Juvenile hormone (JH) and ecdysteroids are the major hormones that regulate molting, metamorphosis and reproduction in insects. Since these hormones are not present in vertebrates, they represent attractive targets for the development of insect control methods. Hindering this effort is the lack of understanding of the molecular basis of JH action. The main objective of this proposal is to identify genes that play critical roles in JH action. JH response elements (JHRE) identified in the promoter region of JH- and 20-hydroxyecdysone (20E)-responsive JH esterase (jhe) gene and ecdysone receptor (EcR) deficient Drosophila melanogaster cell line (L57) were used to develop a robust system that can be used for studying the molecular basis of JH action. In L57 cells, a reporter gene placed under the control of JHRE is induced by JH, and the JH induction was suppressed by 20E. The nuclear proteins isolated from L57 cells bind to JHRE and 20E in the presence of EcR can prevent this binding. In L57 cells, EcR and DSP do not bind directly to JHRE and are not directly involved in JH induction of a reporter gene regulated by JHRE. Identification of proteins that bind to JHRE is critical for understanding JH action. The two specific aims of the proposal are: (1) to identify genes that play key roles in JH action; and (2) to determine the role of identified genes in JH action. The genes that are involved in JH induction of JHRE regulated reporter gene will be identified using RNAi and yeast assays. The identified gene will be characterized by studying the binding of expressed proteins to JHRE and labeled JH, expression of their mRNA and proteins during D. melanogaster development, and analysis of D. melanogaster mutants deficient in identified genes. JH analogs are being used for mosquito control, but some mosquitoes are already developing resistance to these analogs. The results from these studies will aid in understanding the mode of action of JH analogs, thus facilitating their judicious use. The genes identified will be useful to develop screening assays to identify new insect disease vector control agents. The receptors can also be used in medicine by developing gene switches for various applications such as gene therapy, drug discovery and therapeutic protein production.

描述（申请人提供）：有些昆虫与我们竞争食物并传播人类和牲畜疾病，但其他昆虫是有益的。因此，不断需要开发针对特定目标的昆虫控制方法。保幼激素（JH）和蜕皮激素是调节昆虫蜕皮、变态和繁殖的主要激素。由于这些激素不存在于脊椎动物中，因此它们代表了昆虫控制方法开发的有吸引力的目标。阻碍这一努力的是缺乏对 JH 作用的分子基础的了解。该提案的主要目标是确定在 JH 作用中发挥关键作用的基因。在 JH 和 20-羟基蜕皮激素 (20E) 响应的 JH 酯酶 (jhe) 基因和蜕皮激素受体 (EcR) 缺陷的果蝇细胞系 (L57) 的启动子区域中鉴定出的 JH 响应元件 (JHRE) 用于开发一个强大的系统，可用于研究 JH 作用的分子基础。在L57细胞中，JHRE控制下的报告基因被JH诱导，并且JH诱导被20E抑制。从 L57 细胞中分离的核蛋白与 JHRE 结合，而 EcR 存在时 20E 可以阻止这种结合。在L57细胞中，EcR和DSP不直接与JHRE结合，并且不直接参与受JHRE调节的报告基因的JH诱导。鉴定与 JHRE 结合的蛋白质对于理解 JH 作用至关重要。该提案的两个具体目标是：（1）识别在 JH 作用中发挥关键作用的基因； (2) 确定已识别基因在 JH 作用中的作用。将使用 RNAi 和酵母测定来鉴定参与 JHRE 调节报告基因的 JH 诱导的基因。将通过研究表达蛋白与 JHRE 和标记 JH 的结合、黑腹果蝇发育过程中其 mRNA 和蛋白的表达，以及分析缺乏已识别基因的黑腹果蝇突变体来表征已鉴定的基因。 JH 类似物被用于控制蚊子，但一些蚊子已经对这些类似物产生了抗药性。这些研究的结果将有助于理解 JH 类似物的作用方式，从而促进其明智的使用。鉴定出的基因将有助于开发筛选方法来鉴定新的昆虫病媒控制剂。这些受体还可以通过开发用于基因治疗、药物发现和治疗性蛋白质生产等各种应用的基因开关来用于医学。