Tracking immune cell migration in tumour and normal tissues after combined radiation-drug therapy in Kaede mice

追踪 Kaede 小鼠放射药物联合治疗后肿瘤和正常组织中免疫细胞的迁移

• 批准号: 2897116
• 金额: --
• 依托单位: Institute of Cancer Research
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2897116
• 关键词: Tracking; immune; cell; migration; tumour

Kaede mice are transgenic for a photoactivatable fluorescent protein derived from a stony coral, Trachyphyllia geoffroyi. Following in vivo ultraviolet illumination, cells in the light field irreversibly photoconvert from green to red fluorescence. The candidate will use Kaede mice to track migration and activation/suppression of immune cells (by flow cytometry, fluorescence microscopy, transcriptional profiling) in irradiated and unirradiated tissues. The effects of additional immunomodulatory therapies, including chemotherapy, DNA damage response (DDR) and immune checkpoint inhibitors, will also be assessed. The candidate will analyse the effects of dose-fractionation regimens, lymph node irradiation or shielding and spatial dose-modulation on immune cell trafficking.

Kaede小鼠是转基因的光活化荧光蛋白衍生自石珊瑚，Trachyphyllia geoffroyi。在体内紫外线照射后，光场中的细胞不可逆地从绿色荧光光转换为红色荧光。候选人将使用Kaede小鼠跟踪辐照和未辐照组织中免疫细胞的迁移和激活/抑制（通过流式细胞术、荧光显微镜、转录谱分析）。还将评估其他免疫调节治疗的效果，包括化疗、DNA损伤反应（DDR）和免疫检查点抑制剂。候选人将分析剂量分级方案，淋巴结照射或屏蔽和空间剂量调节对免疫细胞运输的影响。