Diagnosis and Tracking of Spinal Staphylococcus aureus Orthopaedic Implant Infections

脊柱金黄色葡萄球菌骨科植入物感染的诊断和追踪

• 批准号: 10554426
• 负责人: Cheryl Lynne Ackert-Bicknell
• 金额: $ 23.18万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-01-24 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10554426
• 关键词: Anatomy; Antibiotic Therapy; Antibodies; Antibody titer measurement; Antibody-Producing Cells; Antigens; B-Cell Activation; Benchmarking; Biological; Biological Assay; Blood; Blood Tests; Blood specimen; Body Temperature; C-reactive protein; Cell Culture Techniques; Cell secretion; Circulation; Clinical; Collecting Cell; Complex; Complication; Cultured Cells; Data; Death Rate; Devices; Diagnosis; Diagnostic; Diagnostic Procedure; Diagnostic tests; Disadvantaged; Early Intervention; Environment; Erythrocyte Sedimentation Rate; Fever; Goals; Harvest; Immune response; Immunoassay; Immunoglobulin G; Immunoglobulin-Secreting Cells; Implant; In Vitro; Infection; Infectious Agent; Infectious Skin Diseases; Joint Prosthesis; Knee; Learning; Liquid substance; Masks; Measurement; Measures; Methods; Microbial Biofilms; Monitor; Morbidity - disease rate; Musculoskeletal; Neurologic Symptoms; Nomograms; Operative Surgical Procedures; Organism; Orthopedics; Outcome; Pain; Patients; Peripheral Blood Mononuclear Cell; Polymers; Postoperative Period; Practice Guidelines; Property; Rapid diagnostics; Reaction; Sampling; Second Look Surgery; Serum; Serum Markers; Site; Spinal; Staphylococcus aureus; Staphylococcus aureus infection; Surface; Surgical Wound Infection; Temperature; Testing; Therapeutic; Therapeutic Intervention; Time; Vertebral column; White Blood Cell Count procedure; Whole Blood; Work; antimicrobial; cost; cytokine; diagnostic accuracy; diagnostic tool; diagnostic value; experience; extracellular; foot; implant associated infection; implantation; in vivo; joint infection; novel; pathogen; prognostic; response; spine bone structure; standard of care; success; treatment guidelines; treatment response; usability

Spinal infections are a serious complication of vertebral implantation surgery with a death rate as high as 20% in the first year. Challenging to identify, especially in the early stages, they are typically diagnosed after becoming well established when the patient is experiencing significant pain and permanent vertebral damage. Here, we propose to explore the early immune response to these infections, focusing on Staphylococcus aureus, (S. aureus), the most frequent and serious among the multiple pathogens that cause spinal implant infections. Our goal is to develop a new way to diagnose spinal implant infections that will: 1) be usable early in the infection's course, 2) cost less than current approaches, 3) require only a routine blood sample, and 4) be capable of monitoring therapeutic success. Most patients have high circulating levels of S. aureus-specific antibodies, and these levels increase with infection, but remain elevated long after the infection has been resolved. To provide a more sensitive diagnostic tool and at the same time create a simple measure for monitoring therapeutic success, we propose to measure the antibodies produced by circulating Antibody- Secreting Cells (ASC) that are present in the blood while the infection is ongoing and rapidly decline thereafter. ASC can be harvested from whole blood, washed free of serum antibodies, and cultured in vitro for a short time. These cultured cells will secrete newly synthesized antibodies, as well as the cytokines, yielding “medium enriched for newly synthesized antibodies” or MENSA, for short. Antibody titers of MENSA fluid against antigens for specific pathogens and the cytokines being secreted can be measured using a multiplex immunoassay. In this proposal, we hypothesize that the diagnosis and monitoring of treatment response due to spine related S. aureus infection is feasible utilizing pathogen-specific antibodies secreted by ASC, and that the antigenic signatures will be distinct compared to other musculoskeletal infections. To test this hypothesis, we will examine MENSA antibody and cytokine levels in patients with a known or suspected infection associated with previously placed spinal orthopedic implants. We will determine if MENSA antibodies alone or in combination with cytokines can discriminate between patients with S. aureus infections or infections due to other pathogens. Accuracy will be determined by comparing to the clinical gold standard of bacterial culture. We will also determine if we can use MENSA to track the response to treatment for S. aureus infection. This study is focused on S. aureus as we can diagnosis this pathogen via culture, giving us a benchmark for accuracy, even though 48% of the time, the infectious agent in spine infections cannot be determined by culture. This study is a proof-of-concept study that can be expanded to other pathogens that are complex to diagnose while also monitoring treatment when direct culture methods become impractical. The goal of this work is the creation of simple, inexpensive, rapid diagnostic method that can lead to earlier interventions and better outcomes for spinal infections patients.

脊柱感染是椎体植入手术的严重并发症，死亡率高达20%