CD8+ T Cell Response to Burn Injury

主要研究者的目标是成为一名独立资助的外科科学家，将基本免疫学转化为严重烧伤和创伤中最具挑战性问题的解决方案。首席研究员有一个强大的初步记录，完成了N1 H-NRSA创伤培训奖学金，并发表了一些科学手稿。该提案旨在实现长期的科学成功，概述了一项具体的培训计划，其中包括教学工作、技术讲习班、直接实验室监督、道德和应用基础科学研究的专业发展。环境：UNC-CH拥有强大的学术传统，并致力于培养临床科学家。学校拥有许多核心设施，包括Lineberger癌症中心和分子生物学和生物技术计划，专门用于支持临床科学家的发展。Jeffrey A.博士Frelinger博士，微生物学和免疫学系主任，本提案的导师，拥有长期的科学成功记录，并专门致力于主要研究者的科学发展。主要研究者将获得成功完成本提案所需的所有实验室设备、资源、专业知识和办公空间。外科部门绝对致力于主要研究者的科学发展，并坚定地承诺将75%的时间用于这项提案。研究：该提案的目标是确定CD 8 + T细胞对烧伤的反应。CD 8 + T细胞，越来越多地被认为是免疫反应的重要调节因子，对烧伤的反应知之甚少。初步数据显示，虽然对烧伤的CTL同种异体反应受损，但活化的CD 8 + T细胞的表型表明过度的促炎反应。该建议的假设是，CD 8 + T细胞对烧伤的反应是多效性的，并且这种反应的机制是改变的CD 8 + T细胞活化。主要研究者将使用各种先进的细胞免疫学技术来确定烧伤后CD 8 + T细胞的表型和功能。此外，将研究CD 8 + T细胞活化的机制，特别是宿主环境和细胞因子环境如何影响CD 8 + T细胞对烧伤的反应。

DESCRIPTION (provided by applicant): Candidate: The goal of the principal investigator is to become an independently funded surgical scientist who translates basic immunology into solutions for the most challenging problems in severe burns and trauma. The principal investigator has a strong preliminary record, having completed a N1H-NRSA trauma training fellowship and published a number of scientific manuscripts. The proposal, designed for long-term scientific success, outlines a specific training plan that includes didactic work, technical workshops, direct laboratory supervision, ethics, and professional development in applied basic science research. Environment: UNC-CH has a strong academic tradition and a commitment to training clinician scientists. The school has a number of core facilities, including the Lineberger Cancer Center and the Program in Molecular Biology and Biotechnology specifically designed to support the development of clinician scientists. Dr. Jeffrey A. Frelinger, PhD, Chair, Department of Microbiology and Immunology, the mentor for this proposal, has a long track record of scientific success and is committed specifically to the scientific development of the principal investigator. All required laboratory equipment, resources, expertise, and office space necessary for successful completion of this proposal will be available to the principal investigator. The Department of Surgery is absolutely committed to the scientific development of the principal investigator and his firm commitment to spend 75% of his time on this proposal. Research: The goal of this proposal is to determine the CD8+ T cell response to burn injury. CD8+ T cells, increasingly recognized as an important regulator of the immune response, have a poorly understood response to burn injury. Preliminary data reveals that while the CTL alloresponse to burn injury is impaired, the phenotype of activated CD8+ T cells suggests an exaggerated proinflammatory response. The hypothesis of this proposal is that the CD8+ T cell response to burn injury is pleiotropic and the mechanism for this response is altered CD8+ T cell activation. The principal investigator will use a variety of advanced cellular immunologic techniques to determine the phenotype and function of CD8+ T cells after burn injury. In addition, the mechanisms of CD8+ T cell activation will be investigated, specifically how the host environment and the cytokine environment influence the CD8+ T cell response to burn injury.