CORE--Cell Culture/Adenovirus

CORE--细胞培养/腺病毒

• 批准号: 7133533
• 负责人: MING TSE
• 金额: $ 10.82万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-15 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7133533
• 关键词: Adenoviridae; Lentivirus; antibody; biomedical facility; cell line; complementary DNA; computers; epithelium; infection; reagent /indicator; tissue /cell culture; tissue resource /registry; training

Core B: The Cell Culture/Adeno/Lentivirus Core is a Service Core which will: 1) Grow and maintain specialized cell lines to be used by multiple investigators. 2. After infection, maintain epithelial cell lines with adenoviral constructs for acute studies and lentiviral constructs. 3. Keep list of all cells maintained in culture computerized. 4. Assign costs to PPG investigators for use of Core resources (provision of cells, adenovirus, lentivirus etc.). 5. Provide small amounts of cultured cells to other investigators both within and outside of Hopkins for pilot experiments (charge-back to be used). 6. Provide cells/reagents of PPG investigators to scientists around Hopkins and outside Hopkins (cells, antibodies, cDNAs, etc.). 7. Although the Core is largely a Service Core, it also will continue to serve a more minor role to train investigators in using epithelial cells in culture. 8. Provide adenovirus constructs for transient infection studied and lentiviral constructs to make stable lines. This requires engineering, growth, purification and titering.

核心B：细胞培养/adeno/lentivirus核心是一种服务核心，它将： 1）生长和维护专门的细胞系，可供多个研究者使用。 2。感染后，用腺病毒构建急性研究和慢病毒的腺病毒构建线维持上皮细胞系 构造。 3。保留在计算机化文化中维持的所有细胞的列表。 4。将成本分配给PPG调查人员使用核心资源（提供细胞，腺病毒，慢病毒 ETC。）。 5。向霍普金斯内外的其他研究人员提供少量的培养细胞，以供飞行员 实验（要使用的充电）。 6。向霍普金斯和霍普金斯外部的科学家提供PPG研究者的细胞/试剂（细胞， 抗体，cDNA等）。 7。尽管核心在很大程度上是服务核心，但它也将继续发挥更小的作用 培训研究人员在培养中使用上皮细胞。 8。提供研究的瞬态感染和慢病毒构建体的腺病毒构建体以使稳定 线。这需要工程，增长，净化和效率。