CORE--MOLECULAR AND CELL BIOLOGY

核心--分子和细胞生物学

• 批准号: 7137089
• 负责人: Anton M Bennett
• 金额: $ 19.48万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7137089
• 关键词: biomedical facility; calcium flux; cell biology; cell nucleus; gene mutation; genetically modified animals; laboratory mouse; liver cells; molecular biology; molecular cloning; nucleic acid amplification techniques; nucleic acid purification; nucleic acid sequence; tissue /cell culture; tissue /cell preparation; tissue resource /registry

The Molecular Biology and Animal Core will provide reagents to assist in the study of nuclear calcium signaling and its effects in liver. The core will generate, purify and maintain adenoviruses and genetically engineered cell lines in which nuclear or cytosolic calcium signaling is suppressed. The core also will assist in the development and characterization of siRNAs requested for use in the individual projects. The core will offer breeding and genotyping services for MKP-1 knock-out mice. The core will provide hepatocytes and other cells from MKP-1 knock-out mice for the analysis of calcium signaling and related studies. The core will include dedicated personnel and will be located within the Core Directors' laboratory.

分子生物学和动物中心将提供试剂来协助核的研究