CORE--Assay

核心检测

• 批准号: 7133570
• 负责人: MATTHIAS H TSCHOP
• 金额: $ 17.14万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7133570
• 关键词: bioassay; biomedical facility; cholecystokinin; corticosterone; gastrin releasing peptide; glucose; immunologic assay /test; insulin; leptin; measurement; obesity; peptide hormone; radioimmunoassay; technology /technique development; tissue /cell preparation

Since the measurement of peptide hormones and circulating substrates will provide key experimental endpoints for each of the separate projects that make up this program project, funding is requested for an Assay Core laboratory. The primary purpose of this laboratory will be to establish and perform assays for the measurement of the key regulatory peptides necessary for the completion of the three projects. Routine assays for the core will include insulin, leptin, glucose, corticosterone, gastrin releasing peptide, and cholecystokinin. This core will also be responsible for adapting commercially available kits necessary to measure other hormones and peptides, labeling peptides for use as tracers, processing tissues for assay of peptides and development of new techniques and assays. This core will be utilized extensively by all 3 of the individual projects.

由于肽激素和循环底物的测量将提供关键的实验 构成该计划项目的每个单独项目的终点，需要资助 测定核心实验室。该实验室的主要目的是建立并进行以下分析： 完成这三个项目所需的关键调节肽的测量。 核心的常规检测包括胰岛素、瘦素、葡萄糖、皮质酮、胃泌素释放肽、 和胆囊收缩素。该核心还将负责调整商用套件 测量其他激素和肽、标记用作示踪剂的肽、处理组织所必需的 用于肽的测定以及新技术和测定的开发。该核心将被利用 广泛地由所有 3 个单独的项目组成。