TSE LabMaster - CaloS-Dri/Fed+AC/Act.XY-Tel, 3x8 Mice

描述（由申请人提供）：肥胖及相关疾病如糖尿病仍然是西方社会主要的健康威胁。为了更好地理解参与体脂调节的代谢过程的分子调控，需要大量的遗传和药理学改变的动物模型，以及一套日益完善的工具来表征这些模型。我们需要一个24笼综合老鼠能量平衡分析系统。主要特点是最先进的开路间接量热仪与高速气体传感器，同时和连续监测食物和水的消耗，运动活动，能量消耗，身体核心温度和心率。重要的是，它在从寒冷暴露到动物热中性区（在那里它们需要尽可能少的代谢能量来冷却或加热它们的生物体）的几种环境温度梯度内执行小鼠代谢表型。此外，该系统还包括一种新颖的方式，通过智能软件来“控制”食物摄入，从而允许对细胞代谢和能量消耗进行独立的喂养研究。我们建议的关键点是，所要求的系统将允许将小鼠模型的能量代谢分析作为这里列出的主要和次要用户的众多赠款和项目的中心部分，达到前所未有的技术复杂程度，这将允许高通量生成更具体，更详细，更相关，更科学和生理上有意义的结果。直接相关的项目将大量受益于这一共享仪器，重点关注中枢神经系统对能量代谢的直接调节，肠道激素-大脑相互作用或已知的调节能量代谢的炎症途径。最终，该仪器及其在辛辛那提大学众多正在进行的代谢和肥胖研究中的应用将促进对肥胖和糖尿病的更好理解。

DESCRIPTION (provided by applicant): Obesity and related disease such as diabetes remain major health threats in western societies. In order to better understand the molecular regulation of metabolic processes involved in body fat regulation, numerous genetically and pharmacologically altered animal models are necessary along with an increasingly refined set of tools to characterize these models. We are requesting a 24 cage Integrated Mouse Energy Balance Analysis system. Key features are state-of-the-art open-circuit indirect calorimetry with high-speed gas sensors, and simultaneous and continuous monitoring of food and water consumption, locomotor activity, energy expenditure, body core temperature and heart rate. Importantly, it performs mouse metabolic phenotyping within a gradient of several environmental temperatures from cold exposure to the animals thermoneutral zone (where they need the least possible metabolic energy to cool or heat their organism). Also, this system includes a novel way to "clamp" food intake by smart software to allow feeding independent study of cell metabolism and energy expenditure. The key point of our proposal is that the requested system will allow to take energy metabolism analysis of mouse models as center parts of numerous grants and projects by the here listed major and minor users to an unprecedented level of technical sophistication that will allow for high- throughput generation of more specific, more detailed, more relevant and more scientifically and physiologically meaningful results. Directly related projects which would massively benefit from this shared instrument are focusing on direct regulation of energy metabolism by the CNS, gut hormone - brain interactions or inflammatory pathways known to also regulated energy metabolism. Ultimately, this instrument and its use for the numerous ongoing metabolism and obesity studies at the University of Cincinnati will facilitate progress towards a better understanding of obesity and diabetes.