TSE LabMaster - CaloS-Dri/Fed+AC/Act.XY-Tel, 3x8 Mice

TSE LabMaster - CaloS-Dri/Fed AC/Act.XY-Tel，3x8 小鼠

• 批准号: 7794000
• 负责人: MATTHIAS H TSCHOP
• 金额: $ 44.99万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2011-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794000
• 关键词: Animal Model; Animals; Arts; Body fat; Brain; Cells; Computer software; Diabetes Mellitus; Eating; Energy Metabolism; Exposure to; Gases; Generations; Grant; Health; Heart Rate; Heating; Hormones; Indirect Calorimetry; Inflammatory; Metabolic; Metabolism; Minor; Modeling; Molecular; Motor Activity; Mus; Obesity; Obesity associated disease; Organism; Pathway interactions; Phenotype; Regulation; Societies; Speed; System; Systems Analysis; Temperature; Universities; Water consumption; benefit sharing; cold temperature; energy balance; feeding; food surveillance; instrument; mouse model; novel; sensor; tool

DESCRIPTION (provided by applicant): Obesity and related disease such as diabetes remain major health threats in western societies. In order to better understand the molecular regulation of metabolic processes involved in body fat regulation, numerous genetically and pharmacologically altered animal models are necessary along with an increasingly refined set of tools to characterize these models. We are requesting a 24 cage Integrated Mouse Energy Balance Analysis system. Key features are state-of-the-art open-circuit indirect calorimetry with high-speed gas sensors, and simultaneous and continuous monitoring of food and water consumption, locomotor activity, energy expenditure, body core temperature and heart rate. Importantly, it performs mouse metabolic phenotyping within a gradient of several environmental temperatures from cold exposure to the animals thermoneutral zone (where they need the least possible metabolic energy to cool or heat their organism). Also, this system includes a novel way to "clamp" food intake by smart software to allow feeding independent study of cell metabolism and energy expenditure. The key point of our proposal is that the requested system will allow to take energy metabolism analysis of mouse models as center parts of numerous grants and projects by the here listed major and minor users to an unprecedented level of technical sophistication that will allow for high- throughput generation of more specific, more detailed, more relevant and more scientifically and physiologically meaningful results. Directly related projects which would massively benefit from this shared instrument are focusing on direct regulation of energy metabolism by the CNS, gut hormone - brain interactions or inflammatory pathways known to also regulated energy metabolism. Ultimately, this instrument and its use for the numerous ongoing metabolism and obesity studies at the University of Cincinnati will facilitate progress towards a better understanding of obesity and diabetes.

描述(申请人提供)：肥胖和相关疾病，如糖尿病，仍然是西方社会的主要健康威胁。为了更好地了解参与体内脂肪调节的代谢过程的分子调节，需要大量的遗传和药物改变的动物模型，以及一套日益完善的工具来表征这些模型。我们要求一套24笼集成鼠标能量平衡分析系统。主要功能是具有高速气体传感器的最先进的开路间接热量测量，以及对食物和水消耗、运动活动、能量消耗、身体核心温度和心率的同步和持续监测。重要的是，它在几个环境温度的梯度内执行小鼠的代谢表型，从冷暴露到动物的中温区(在那里它们需要尽可能少的新陈代谢能量来冷却或加热他们的有机体)。此外，该系统还包括一种新颖的方式，通过智能软件来“夹住”食物的摄入量，允许喂食独立研究细胞新陈代谢和能量消耗。我们建议的关键点是，所要求的系统将允许将鼠标模型的能量代谢分析作为此处列出的主要用户和次要用户的众多赠款和项目的中心部分，达到前所未有的技术复杂程度，从而允许高通量生成更具体、更详细、更相关、更具科学和生理意义的结果。将从这一共享工具中大量受益的直接相关项目侧重于通过中枢神经系统直接调节能量代谢、肠道激素-大脑相互作用或已知也调节能量代谢的炎症途径。最终，该仪器及其在辛辛那提大学正在进行的众多新陈代谢和肥胖研究中的使用将有助于在更好地了解肥胖和糖尿病方面取得进展。