CORE--Assay

核心检测

• 批准号: 7425079
• 负责人: MATTHIAS H TSCHOP
• 金额: $ 17.18万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7425079
• 关键词: Biological Assay; Cholecystokinin; Corticosterone; Development; End Point; Funding; Gastrin releasing peptide; Glucose; Individual; Insulin; Label; Laboratories; Leptin; Measurement; Measures; Peptides; Purpose; Techniques; Tracer; peptide hormone; programs; tissue processing

Since the measurement of peptide hormones and circulating substrates will provide key experimental endpoints for each of the separate projects that make up this program project, funding is requested for an Assay Core laboratory. The primary purpose of this laboratory will be to establish and perform assays for the measurement of the key regulatory peptides necessary for the completion of the three projects. Routine assays for the core will include insulin, leptin, glucose, corticosterone, gastrin releasing peptide, and cholecystokinin. This core will also be responsible for adapting commercially available kits necessary to measure other hormones and peptides, labeling peptides for use as tracers, processing tissues for assay of peptides and development of new techniques and assays. This core will be utilized extensively by all 3 of the individual projects.

由于测量肽激素和循环底物将提供关键的实验 每个组成该计划项目的独立项目的终点，要求资金用于 测定核心实验室。该实验室的主要目的是建立和执行测定 完成三个项目所需的关键调节肽的测量。 核心的常规测定法包括胰岛素，瘦素，葡萄糖，皮质酮，胃蛋白释放肽， 和胆囊动蛋白。该核心还将负责调整市售套件 测量其他激素和肽，将肽用作示踪剂的肽所需的必要 用于测定肽以及新技术和测定的开发。此核心将被使用 所有三个项目都广泛地。