NIR Tunable Laser Tissue Welding

近红外可调谐激光组织焊接

• 批准号: 7234338
• 负责人: Robert Richard. Alfano
• 金额: $ 31.13万
• 依托单位: CITY COLLEGE OF NEW YORK
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7234338
• 关键词: Affect; Animal Model; Animals; Birefringence; CD11c Antigens; Cavia; Cicatrix; Clinical; Closure; Collagen; Deposition; Development; Elastin; Electron Microscopy; Electronics; Healed; Heat Waves; Heating; Histology; Histopathology; Hydration status; Infection; Knowledge; Lasers; Lead; Life; Measurement; Methodology; Methods; Microsurgery; Molecular; Monitor; Necrosis; Object Attachment; Operative Surgical Procedures; Outcome; Output; Physiologic pulse; Physiological; Physiological Processes; Plastic Surgical Procedures; Play; Postoperative Period; Process; Pulse taking; Pump; Raman Spectrum Analysis; Rate; Relaxation; Research; Research Project Grants; Risk; Role; Scanning Transmission Electron Microscopy Procedures; Skin Tissue; Structure; Surgical incisions; Surgical sutures; System; Tensile Strength; Thick; Time; Tissues; Translations; Water; Welding; Width; Wound Healing; absorption; base; day; healing; improved; in vivo; optical fiber; research study; response; solid state; tissue welding; transmission process; vibration; wound

DESCRIPTION (provided by applicant): The long-term objectives of this competing continuation application are to: (1) Develop a compact, Cr4+- based tunable laser and optical fiber beam delivery system suitable for in vivo laser tissue welding animal studies, with potential for translation into clinical settings; (2) Develop a detailed understanding of the molecular mechanism behind LTW that includes the roles of thermal and non-thermal processes; (3) Improve welding efficacy by optimizing laser parameters, in particular, laser pulse-width, heat management, and buckling control; and (4) Evaluate the effect of physiological processes on welding and wound healing by extending LTW to living tissues using an animal model, and develop appreciation of its clinical potential and scope. To accomplish the long-term objectives, the specific aims of the research are to: (1) Develop a compact, all-solid-state laser pumped continuous wave (CW) Cr4+:YAG laser and modelock it to generate picosecond and femtosecond pulses, as well as, an optical fiber system to deliver the laser beam to the target.tissue to be welded; (2) Investigate changes in hydration and structure of collagen and elastin in ex vivo tissues as a result of welding, using Raman spectroscopy; polarized transmission (birefringence); histology; tensile strength analysis, and scanning and transmission electron microscopy; (3) Compare effects of laser pulse width (ps, fs and CW), heat management, and control of buckling on welding efficacy to determine whether short pulses can reduce collateral damage and improve bond strength in comparison to thermal processes; and (4) Extend the experimental approach, expertise, and knowledge of key LTW parameters to a carefully chosen animal model - albino guinea pigs - to investigate in vivo the wound healing response following LTW. The healing process and efficacy of welding will be evaluated by monitoring the degree and rate of epithelialization, extent of damage, granulation and collagen deposition, thickness of necrotic tissue sloughed off, degree of tissue apposition, and tensile strength of the welds on guinea pigs, over a 42-day period. The development of leak-proof, sutureless methods of healing surgical incisions will reduce risks of post-operative infection and scarring and provide an improved methodology for wound closure in microsurgery and endoscopic surgery. In addition, the use of ps and fs lasers for LTW is expected to result in reduced collateral damage and stronger tissue fusion. The outcome of the proposed research will have profound impact on wound healing and plastic surgery.

描述（由申请人提供）： 这一竞争性延续申请的长期目标是：（1）开发一种适用于体内激光组织焊接动物研究的紧凑型、基于Cr 4+的可调谐激光和光纤光束传输系统，并有可能转化为临床环境;（2）详细了解LTW背后的分子机制，包括热过程和非热过程的作用;（3）通过优化激光参数，特别是激光脉冲宽度、热管理和屈曲控制，提高焊接效率;以及（4）通过使用动物模型将LTW扩展到活组织，评估生理过程对焊接和伤口愈合的影响，并对其临床潜力和范围进行评估。 为了实现长期目标，本研究的具体目标是：（1）开发一种紧凑的全固态激光泵浦连续波（CW）Cr 4+：YAG激光器，并将其锁模以产生皮秒和飞秒脉冲，以及将激光束传送到待焊接的目标组织的光纤系统;（2）使用拉曼光谱法研究由于焊接而导致的离体组织中胶原蛋白和弹性蛋白的水合和结构的变化;极化传输（双折射）;组织学;拉伸强度分析，扫描和透射电子显微镜;（3）比较激光脉冲宽度（ps、fs和CW）、热管理和屈曲对焊接功效的控制，以确定与热处理相比，短脉冲是否可以减少附带损伤并提高结合强度;和（4）将实验方法、专业知识和关键LTW参数的知识扩展到精心选择的动物模型-白化病豚鼠-以研究LTW后的体内伤口愈合反应。 在42天的时间内，通过监测上皮形成的程度和速率、损伤程度、肉芽和胶原沉积、坏死组织脱落的厚度、组织贴壁程度和豚鼠焊接的抗拉强度，评价焊接的愈合过程和有效性。开发防漏、无缝线的手术切口愈合方法将降低术后感染和瘢痕形成的风险，并为显微外科和内窥镜手术中的伤口闭合提供改进的方法。此外，预期使用ps和fs激光进行LTW可减少附带损伤并增强组织融合。这项研究的结果将对伤口愈合和整形手术产生深远的影响。

项目成果

Laser tissue welding analyzed using fluorescence, Stokes shift spectroscopy, and Huang-Rhys parameter.

使用荧光、斯托克斯位移光谱和 Huang-Rhys 参数分析激光组织焊接。

• DOI: 10.1002/jbio.201100050
• 发表时间: 2012
• 期刊: Journal of biophotonics
• 影响因子: 2.8
• 作者: Sriramoju,Vidyasagar;Alfano,RobertR
• 通讯作者: Alfano,RobertR

In vivo studies of ultrafast near-infrared laser tissue bonding and wound healing.

超快近红外激光组织粘合和伤口愈合的体内研究。

• DOI: 10.1117/1.jbo.20.10.108001
• 发表时间: 2015
• 期刊: Journal of biomedical optics
• 影响因子: 3.5
• 作者: Sriramoju,Vidyasagar;Alfano,RobertR
• 通讯作者: Alfano,RobertR

In vivo molecular evaluation of guinea pig skin incisions healing after surgical suture and laser tissue welding using Raman spectroscopy.

• DOI: 10.1016/j.jphotobiol.2009.06.004
• 发表时间: 2009-09-04
• 期刊: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
• 影响因子: 5.4
• 作者: Alimova, A.;Chakraverty, R.;Muthukattil, R.;Elder, S.;Katz, A.;Sriramoju, V.;Lipper, Stanley;Alfano, R. R.
• 通讯作者: Alfano, R. R.

Management of heat in laser tissue welding using NIR cover window material.

使用 NIR 盖窗材料进行激光组织焊接中的热量管理。

• DOI: 10.1002/lsm.21143
• 发表时间: 2011
• 期刊: Lasers in surgery and medicine
• 影响因子: 2.4
• 作者: Sriramoju,Vidyasagar;Savage,Howard;Katz,Alvin;Muthukattil,Ronex;Alfano,RobertR
• 通讯作者: Alfano,RobertR