Commercialization of Embryonic Kidney Cell Lines

描述（由申请人提供）：肾脏疾病研究迫切需要增加资源和人力。Probetex成立时是一家“肾脏研究资源公司”，旨在创建一个“现成”组织产品库，以提供随时可用的样本，并帮助研究人员快速有效地探索他们对已建立和标准化模型或已确定的人类疾病病例的想法。该目标的一部分是建立一个肾脏细胞系库，以补充其肾脏组织产品组合。事实上，肾祖细胞的分离、克隆、维持和表征在国家糖尿病、消化和肾病研究所和美国肾脏学会理事会赞助的战略规划会议的报告中被确定为研究重点。这些优先事项包括以下举措：1）开发新的器官型体外培养模型和2）开发允许干细胞定向为肾祖细胞的条件，然后维持这些分化的细胞系。该I期申请的基础（与圣安东尼奥的德克萨斯大学健康科学中心合作）是通过定义来自小鼠和大鼠胚胎原基的三种现有肾祖细胞系的生长和分化条件来解决上述优先事项，并从发育中的胚胎肾中分离和表征新的细胞系。具体目标是：目标1。目的：研究肾原基来源的胚胎后肾间充质（MM）和输尿管芽（UB）细胞系的分化潜能。分化条件包括在共培养物中、在专门的条件培养基中以及在将细胞单独地和组合地植入SCID小鼠中之后的生长。将使用免疫组织化学和RT-PCR进行表达分析，以验证特定细胞类型和细胞分化状态的扩展表达标志物集。目标二。分离和表征来自胚胎后肾肾的肾发生区的新胚胎细胞系，并使用上述标准表征细胞;目的3和4旨在建立胚胎肾细胞库和商业化计划，用于扩增新细胞系、肾细胞繁殖的大规模生产方案、外包，和许可，旨在扩大Probetex储存库中可供公司研究者/客户使用的细胞系的范围。

DESCRIPTION (provided by applicant): There is a crucial need for increases in resources and manpower in research in kidney disease. Probetex was founded as a "Renal Research Resource Company" to create a repository of "off the-shelf" tissue products to provide readily available samples and help investigators to quickly and efficiently explore their ideas on established and standardized models or identified cases of human disease. Part of this goal is to create a repository of kidney cell lines to supplement its portfolio of renal tissue products. Indeed, the isolation, cloning, maintenance, and characterization of renal progenitor cells is identified as a research priority in a Report of the Strategic Planning Conferences sponsored by the National Institute of Diabetes and Digestive and Kidney Diseases and the Council of American Kidney Societies. Included in these priorities are initiatives to 1) develop new organotypic in vitro culture models and 2) develop conditions to permit commitment of stem cells into renal progenitors, and then the maintenance of these differentiated cell lines. The basis of this Phase I application (in collaboration with the University of Texas Health Science Center at San Antonio) is to address the above priorities by defining growth and differentiation conditions for three existing kidney progenitor cell lines derived from mouse and rat embryonic primordia and to isolate and characterize new cell lines from developing embryonic kidney. Specific aims are: Aim 1. To characterize the differentiation potential of embryonic metanephric mesenchymal (MM) and ureteric bud (UB) cell lines derived from kidney primordia. Differentiation conditions include growth in co-culture, in specialized conditioned media, and after implantation of the cells individually and in combination in SCID mice. Expression analysis will be conducted using immunohistochemistry and RT-PCR to validate expanded set of expression markers for specific cell types and differentiation states of the cells. Aim 2. To isolate and characterize new embryonic cell lines derived from the nephrogenic zone of embryonic metanephric kidney and characterize the cells using criteria as outlined above; Aims 3 and 4 are designed to establish an Embryonic Kidney Cell Bank and Commercialization Plan for expansion of new cell lines, large-scale production protocols for kidney cell propagation, out-sourcing, and licensing with the intent to expand the scope of cell lines in the Probetex's repository that are available to the company's investigator/ customers.