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In-Vivo Microscopy for Tissue Engineering

用于组织工程的体内显微镜

基本信息

批准号：
7230228
负责人：
STANISLAV Y EMELIANOV
金额：
$ 18.21万
依托单位：
UNIVERSITY OF TEXAS AT AUSTIN
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-06-01 至 2009-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7230228
关键词：
3-Dimensional Address Algorithms Anatomy Animals Architecture Biomechanics Cell physiology Custom Data Development Diagnostic Imaging Dimensions Elasticity Elements Engineering Frequencies Functional Imaging Goals Growth Image Imagery Imaging Device Imaging technology Invasive Laboratories Lasers Life Light Microscope Microscopic Microscopy Microsurgery Modality Molecular Conformation Monitor Natural regeneration Performance Property Range Rate Research Research Personnel Research Project Grants Resolution Risk Scanning Signal Transduction Source Structure Surface System Technology Testing Time Tissue Engineering Tissue Sample Tissues Transducers Ultrasonic Transducer Ultrasonics Ultrasonography United States National Institutes of Health Week Work anticancer research base blood perfusion cellular imaging day design image processing imaging probe in vivo insight millimeter millisecond novel optical fiber performance tests programs prototype success time use tool ultrasound imaging/scanning viscoelasticity

项目摘要

DESCRIPTION (provided by applicant): With the great success of tissue engineering over the past decade, there is a definite and urgent need to image the engineered living tissues in a qualitative and quantitative manner. The overall goal of our research program is to develop an advanced in-vivo imaging technology; namely, combined ultrasound, photoacoustic and elasticity microscopy, capable of visualizing both the structural and functional properties of living tissue such as internal micro- and macro-architecture, surface topography, conformation, transformation, compliance, homogeneity, growth rate, biomechanics and even cell function within tissues. The underlying hypothesis of this project is that remote, non-invasive, high-frequency, high-resolution, in-vivo microscopy is possible and will provide marked advantages over existing imaging tools available for tissue engineers. The fundamental premise of our research program is to develop an advanced in-vivo microscopy based on the fusion of three complementary imaging modalities - ultrasound, photoacoustics, and elastography - and to take full advantage of the many synergistic features of these systems, thus providing a much needed quantitative imaging tool to tissue engineers. Indeed, ultrasound-based imaging on the microscopic scale offers a conceptually and technically novel imaging tool for tissue engineering. The main objective of this application is to develop a prototype of the high-resolution, multifunctional microscope for tissue engineers. To achieve our objective, we will design and build the combined ultrasound- based microscopy system based on a mechanically scanned, single element transducer interfaced with a laser source. We will also develop algorithms for ultrasound, photoacoustic and elasticity imaging to optimize the performance of the combined system. We will then test the developed microscope and corresponding signal and image processing algorithms using tissue mimicking phantoms. Finally, based on the insights gathered during the project, we will outline the design and technical specifications of an in-vivo microscopy system. The long-range goal of our research program is to develop a combined ultrasound-based microscopy system for tissue engineers.

描述（由申请人提供）：随着过去十年组织工程的巨大成功，迫切需要以定性和定量的方式对工程活组织进行成像。我们研究项目的总体目标是开发先进的体内成像技术；即结合超声、光声和弹性显微镜，能够可视化活体组织的结构和功能特性，例如内部微观和宏观结构、表面形貌、构象、转变、顺应性、均匀性、生长速率、生物力学，甚至组织内的细胞功能。该项目的基本假设是，远程、非侵入性、高频、高分辨率的体内显微镜是可能的，并且将比组织工程师现有的成像工具具有显着的优势。我们研究计划的基本前提是开发一种基于超声波、光声学和弹性成像这三种互补成像模式融合的先进体内显微镜，并充分利用这些系统的许多协同特征，从而为组织工程师提供急需的定量成像工具。事实上，基于超声的微观成像为组织工程提供了概念上和技术上新颖的成像工具。该应用的主要目标是为组织工程师开发高分辨率、多功能显微镜的原型。为了实现我们的目标，我们将设计和构建基于机械扫描的单元件换能器与激光源连接的组合超声显微系统。我们还将开发超声波、光声和弹性成像算法，以优化组合系统的性能。然后，我们将使用模仿组织的模型来测试开发的显微镜以及相应的信号和图像处理算法。最后，根据项目期间收集的见解，我们将概述体内显微镜系统的设计和技术规格。我们研究计划的长期目标是为组织工程师开发一种基于超声的组合显微镜系统。