Design and evolution of metalloenzymes for efficient and selective CO2 reduction

该项目将在PI实验室开发的第一代从头二氧化碳还原酶的基础上，创造出用于高效和选择性地将二氧化碳转化为一氧化碳和商品化学品的从头酶。对这些从头酶的表征将提供对活性中心特征和控制二氧化碳还原和转化的催化机理的详细了解，从而为下一代催化剂的设计奠定基础。这些雄心壮志将通过在计算酶设计、结构生物学、遗传密码扩展、定向进化和生物物理酶表征方面使用最先进的方法来实现。

This project will create de novo enzymes for efficient and selective conversion of CO2 into CO and commodity chemicals, building from a first generation de novo CO2 reductase enzymes developed in the PI lab. Characterization of these de novo enzymes will provide a detailed understanding of the active site features and catalytic mechanisms controlling CO2 reduction and transformation to underpin the design of future generations of catalysts. These ambitions will be achieved by employing state-of-the-art methods in computational enzyme design, structural biology, genetic code expansion, directed evolution and biophysical enzyme characterization.