Temperature-Modulated Bioluminescence Tomography

温度调制生物发光断层扫描

• 批准号: 7234256
• 负责人: Ge Wang
• 金额: $ 19.53万
• 依托单位: VIRGINIA POLYTECHNIC INST AND ST UNIV
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7234256
• 关键词: Affect; Algorithms; Anatomy; Animals; Area; Arts; Bioluminescence; Body Surface; Data; Dependence; Detection; Development; Disease model; Elements; Equilibrium; Feasibility Studies; Focused Ultrasound Therapy; Genes; Goals; Heating; Image; Imaging Techniques; Knowledge; Life; Localized; Measurement; Measures; Modality; Modeling; Molecular Medicine; Mus; Optics; Pattern; Performance; Play; Preventive; Protocols documentation; Reporting; Role; Sampling; Signal Transduction; Simulate; Solutions; Source; System; Techniques; Technology; Temperature; Testing; Time; Transducers; Ultrasonography; United States National Institutes of Health; Validation; absorption; base; density; design; human disease; image reconstruction; improved; in vivo; indexing; interest; molecular imaging; mouse model; pressure; prototype; reconstruction; research study; simulation; success; tomography

DESCRIPTION (provided by applicant): Bioluminescence tomography (BLT) we invented in 2002 is a rapidly developing optical molecular imaging modality for small animal imaging. The state-of-the art results have indicated that BLT can indeed produce valuable tomographic information in favorable cases or with strong prior knowledge. However, the primary challenge in this area remains that it is highly desirable but extremely difficult to stabilize and improve the current BLT performance. It has been recently reported that bioluminescent spectra are significantly affected by temperature, which we recognize as a major opportunity to transform the ill-posed BLT problem into a well-posed setting. Specifically, with focused ultrasound heating, a bioluminescent source distribution in a mouse can be precisely perturbed in a local and controllable fashion. The resultant difference in the externally captured optical signals is solely related to the source parameters in the heated region. Our hypothesis is that this temperature dependence of the bioluminescence can be utilized in the BLT reconstruction for superior image quality. Our overall goal is to develop temperature-modulated bioluminescence tomography (TBT) for mouse studies. The three specific aims are to (1) prototype a focused ultrasound array to heat one small volume of interest (VOI) each time in a living mouse, (2) develop a BLT algorithm to reconstruct an underlying source distribution based on the bioluminescent data measured before and after heating, (3) evaluate the TBT technology in numerical simulation and phantom experiments, as well as apply and validate it in mouse studies. Upon the completion of this project, we will have built the first TBT system and demonstrated its in vivo validity and utility. This success will represent a major step forward in the field of BLT. Since bioluminescent imaging has been used in almost all the mouse models of human diseases, our proposed TBT technology shall have a general, important and immediate applicability in mouse studies, and for development of molecular medicine. Bioluminescence tomography (BLT) invented by us in 2002 is to localize and quantify bioluminescent sources in studies of small animals as many human disease models. This project will improve significantly the accuracy and robustness of the BLT techniques by means of focused ultrasound heating to induce bioluminescent signal changes on the mouse body surface. Our technology shall have a general, important and immediate applicability in mouse studies, and facilitate the development of molecular medicine.

描述（由申请人提供）：我们于2002年发明的生物发光断层扫描（BLT）是一种快速发展的用于小动物成像的光学分子成像模式。最先进的结果表明，在有利的情况下或在有很强的先验知识的情况下，BLT确实可以产生有价值的断层扫描信息。然而，该领域的主要挑战仍然是，稳定和改善当前BLT性能是非常可取的，但极其困难。最近有报道说，生物发光光谱显着受温度的影响，我们认识到这是一个重大的机会，将不适定的BLT问题转化为一个良好的设置。具体地，利用聚焦超声加热，可以以局部和可控的方式精确地扰动小鼠中的生物发光源分布。在外部捕获的光信号中的所得差异仅与加热区域中的源参数有关。我们的假设是，生物发光的这种温度依赖性可以用于BLT重建，以获得上级图像质量。我们的总体目标是开发用于小鼠研究的温度调制生物发光断层扫描（TBT）。这三个具体目标是：（1）制作聚焦超声阵列的原型，每次在活体小鼠中加热一个小的感兴趣体积（VOI），（2）开发BLT算法，根据加热前后测量的生物发光数据重建底层源分布，（3）在数值模拟和体模实验中评估TBT技术，以及在小鼠研究中应用和验证它。在这个项目完成后，我们将建立第一个TBT系统，并证明其在体内的有效性和实用性。这一成功将标志着BLT领域向前迈出了一大步。由于生物发光成像已被用于几乎所有的人类疾病的小鼠模型，我们提出的TBT技术应具有普遍的，重要的和直接的适用性，在小鼠的研究，并为分子医学的发展。生物发光层析成像（BLT）是我们在2002年发明的一种用于小动物研究的生物发光源定位和定量的方法。该项目将通过聚焦超声加热诱导小鼠体表生物发光信号变化，显著提高BLT技术的准确性和鲁棒性。我们的技术将在小鼠研究中具有普遍、重要和直接的应用性，并促进分子医学的发展。