Acquisition of a 500nm Resolution Nano-CT Scanner

购置 500nm 分辨率 Nano-CT 扫描仪

• 批准号: 7595618
• 负责人: Ge Wang
• 金额: $ 50万
• 依托单位: VIRGINIA POLYTECHNIC INST AND ST UNIV
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-15 至 2009-12-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595618
• 关键词: Animal Organ; Animals; Area; Arts; Award; Biological; Biological Markers; Caliber; Clinic; Development; Disease; Disease Progression; Equipment; Funding; Goals; Hydration status; Image; Imaging Device; Inflammation; Inflammatory; Medical; Modeling; Molecular; Monitor; Operative Surgical Procedures; Optics; Pathogenesis; Penetration; Phase; Physiological; Preparation; Prize; Radiation; Research; Research Personnel; Resolution; Sampling; Screening procedure; Societies; Source; Specimen; System; Time; Tissue Sample; Tissues; United States National Institutes of Health; Virginia; Work; bioimaging; cellular imaging; charge coupled device camera; cost; forest; medical schools; mouse model; nano; nanomedicine; public health relevance; response; skeletal

DESCRIPTION (provided by applicant): Over past decades, x-ray CT has revolutionized many aspects of our society especially biomedical imaging. Recently, advances in x-ray optics have enabled x-ray nano-CT with 50-500nm resolution and its wide range of applications. Among modern 5m/nm-scale imaging tools, nano-CT fills in an important gap in image resolution, contrast mechanism, penetration depth, sample preparation and application territory. From biomedical perspective, nano-CT is advantageous in screening of small animals, imaging of physiological and pathological features, and monitoring of disease progression and response to therapy. The overall goal of this project is to acquire a state-of-the-art nano-CT system for a large group of NIH/NSF- funded major users on the Virginia Tech and Wake Forest Campuses to enable or accelerate their research efforts in various biomedical areas. Specifically, in the inflammation studies, Virginia Tech is undergoing a major expansion focusing on inflammation. The newly funded Carilion Clinics and Virginia Tech Carilion School of Medicine have also placed a major emphasis on inflammation. On the campus of Virginia Tech alone, we have at least a dozen highly productive NIH investigators working synergistically on inflammation. One unifying theme underlying most of their research is to visualize the inflamed tissues in the 5m/sub-5m domain, and determine the molecular pathogenesis of inflammatory diseases in mice models. In the nano- medicine research, the requested system is capable of imaging deeply-embedded 5m-sized features of samples non-invasively to maintain specimen integrity and hydration, minimize radiation damage, and reduce operation time and system cost. Also, we have NIH-funded projects involving vasculature modeling and analysis, cellular imaging of biomarkers for skeletal developmental and other studies. The requested system is made by the leading nano-CT company Xradia. This product was awarded the prestigious R&D100 Prize in 2003. The specifications include 90kV micro-focus source, 2kx2k CCD camera, multiple resolution modes down to 500nm, phase-enhanced optics, and up to 100mm diameter samples. It will be highly complementary and synergic with a variety of the 5u-/nm-scale imaging equipment in Virginia Tech. Also, it will be one of few nano-CT systems on the East Coast, and will most likely serve the entire eastern US. PUBLIC HEALTH RELEVANCE: This project is to acquire a state-of-the-art nano-CT scanner that resolves features down to 500nm (which is also referred to as "500nm resolution"), as compared to medical CT scanners of up to 0.3mm resolution and typical micro-CT scanners of up to 55m resolution. This nano-CT capability enables imaging studies of small animal organs and biological tissue samples at a cellular level, and will significantly benefit many leading investigators on the Virginia Tech and Wake Forest campuses.

描述（由申请人提供）：在过去的几十年里，X射线CT已经彻底改变了我们社会的许多方面，特别是生物医学成像。最近，X射线光学的进步使得具有50- 500 nm分辨率的X射线纳米CT及其广泛的应用成为可能。在现代5 m/nm级成像工具中，纳米CT填补了图像分辨率、对比机制、穿透深度、样品制备和应用领域的重要空白。从生物医学的角度来看，纳米CT在筛选小动物、成像生理和病理特征、监测疾病进展和治疗反应方面具有优势。该项目的总体目标是为弗吉尼亚理工大学和维克森林校区的大量NIH/NSF资助的主要用户获得最先进的纳米CT系统，以实现或加速他们在各种生物医学领域的研究工作。具体来说，在炎症研究中，弗吉尼亚理工大学正在进行一项主要的扩张，重点是炎症。新资助的Carpal诊所和弗吉尼亚理工大学Carpal医学院也把重点放在炎症上。仅在弗吉尼亚理工大学的校园里，我们就有至少12名高效的NIH研究人员协同研究炎症。他们大多数研究的一个统一主题是可视化5 m/sub-5 m域中的炎症组织，并确定小鼠模型中炎症性疾病的分子发病机制。在纳米医学研究中，所要求的系统能够非侵入性地对样品的深度嵌入的5米大小的特征进行成像，以保持样品的完整性和水合作用，最大限度地减少辐射损伤，并减少操作时间和系统成本。此外，我们有NIH资助的项目，涉及血管建模和分析，骨骼发育和其他研究的生物标志物的细胞成像。所要求的系统由领先的纳米CT公司Xradia制造。该产品于2003年获得著名的R & D 100奖。规格包括90 kV微聚焦光源、2kx 2k CCD相机、低至500 nm的多种分辨率模式、相位增强光学器件和直径高达100 mm的样品。它将与弗吉尼亚理工大学的各种5 u/nm级成像设备高度互补和协同。此外，它将是东海岸为数不多的纳米CT系统之一，很可能服务于整个美国东部。 公共卫生相关性：该项目旨在获得最先进的纳米CT扫描仪，与分辨率高达0.3 mm的医用CT扫描仪和分辨率高达55 m的典型微型CT扫描仪相比，该扫描仪可分辨低至500 nm的特征（也称为“500 nm分辨率”）。这种纳米CT能力使小动物器官和生物组织样本在细胞水平上的成像研究，并将显着受益于弗吉尼亚理工大学和维克森林校园的许多领先的研究人员。

项目成果

Scout-view assisted interior micro-CT.

• DOI: 10.1088/0031-9155/58/12/4297
• 发表时间: 2013-06-21
• 期刊: Physics in medicine and biology
• 影响因子: 3.5
• 作者: Sen Sharma K;Holzner C;Vasilescu DM;Jin X;Narayanan S;Agah M;Hoffman EA;Yu H;Wang G
• 通讯作者: Wang G