Capillary Electrophoresis and Protein Biophysics

毛细管电泳和蛋白质生物物理学

• 批准号: 7262943
• 负责人: GEORGE M WHITESIDES
• 金额: $ 34.2万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7262943
• 关键词: Acylation; Adsorption; Affinity; Affinity Chromatography; Area; Binding; Biochemical; Biochemistry; Biological; Biological Models; Biophysics; Bioterrorism; Buffers; Calorimetry; Capillary Electrophoresis; Charge; Chemicals; Clinical; Complex; Development; Diagnosis; Disease; Drug Design; Electrophoresis; Event; Funding; Gel; Hydrophobicity; Laboratories; Ligand Binding; Ligands; Membrane; Membrane Proteins; Methods; Modeling; Modification; Molecular Biology; Molecular Weight; Pattern; Plastics; Polymers; Precipitation; Predisposition; Process; Property; Protein Chemistry; Protein Region; Proteins; Proteomics; Public Health; Purpose; Range; Research; Science; Screening procedure; Set protein; Solutions; Solvents; Structure; Surface; Surface Plasmon Resonance; System; Systems Biology; Techniques; Therapeutic Uses; Time; Ubiquitin; Urea; Vision; Work; base; carbonate dehydratase; design; human disease; improved; in vivo; micro-total analysis system; numb protein; physical property; poly(dimethylsiloxane); protein purification; response; surfactant; tool

DESCRIPTION (provided by applicant): This research is focused on the development of new techniques, based on the hydrophobicity of their surfaces, for separating, characterizing, and manipulating proteins. (The proposal is an extensively revised version of a previous proposal that was not funded.) Improving the methods used to separate, characterize, and manipulate proteins is broadly important in biochemistry and molecular biology: areas of application include proteomics and systems biology, purification of protein therapeutics, use in lab-on-a-chip analytical systems for clinical analyses, rational drug design, and fundamental protein science. The proposed research has five specific objectives: 1. To develop a new, validated model system for studying the biophysics of hydrophobic regions of protein surfaces. 2. To develop new techniques for characterization of proteins based on their susceptibility to denaturation by hydrophobic surfactants and hydrotropes (e.g., SDS, not urea). 3. To rationalize systems that use hydrophobic surfactants in manipulating proteins. 4. To provide a solution to the problem of adsorption of proteins on the hydrophobic walls of microchannels in plastic LoC systems. 5. To help to understand the influence of hydrophobicity and organic character of the medium on binding of ligands to proteins. Lay Description: This research will contribute to the analysis and purification of proteins used in treatment of human disease, and to the development of instrumental techniques useful in the diagnosis of disease, in screening for public health, and in response to biological events (emerging disease, bioterrorism).

描述（由申请人提供）：本研究的重点是开发新技术，基于其表面的疏水性，用于分离，表征和操纵蛋白质。(The该提案是对先前未获得资助的提案的广泛修订版本）。改进用于分离、表征和操纵蛋白质的方法在生物化学和分子生物学中具有广泛的重要性：应用领域包括蛋白质组学和系统生物学、蛋白质治疗剂的纯化、用于临床分析的芯片实验室分析系统、合理的药物设计和基础蛋白质科学。本研究有五个具体目标：1。建立一个新的、经过验证的模型系统，用于研究蛋白质表面疏水区域的生物物理学。2.根据蛋白质对疏水表面活性剂和水溶助长剂（例如，SDS，不是尿素）。3.使使用疏水表面活性剂操纵蛋白质的系统合理化。4.为解决塑料LoC系统中蛋白质在微通道疏水壁上的吸附问题提供了一种解决方案。5.帮助理解介质的疏水性和有机性对配体与蛋白质结合的影响。 铺设说明：这项研究将有助于分析和纯化用于治疗人类疾病的蛋白质，并有助于开发用于疾病诊断、公共卫生筛查和应对生物事件（新出现的疾病、生物恐怖主义）的仪器技术。