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VISCOELASTIC PROPERTIES OF NORMAL AND OA CHONDRONS

正常软骨和 OA 软骨的粘弹性特性

基本信息

批准号：
7172926
负责人：
Farshid Guilak
金额：
$ 32.85万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
起止时间：
1998-01-01 至 2008-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7172926
关键词：
Abbreviations Adult Affect Alginates Anabolism Animals Atomic Force Microscopy Behavior Biochemical Biomechanics Cartilage Cells Characteristics Chondrocytes Collagen Collagen Type VI Complex Condition Confocal Microscopy Degenerative polyarthritis Development Diffusion Elements Environment Environmental Risk Factor Equilibrium Exhibits Extracellular Matrix Finite Element Analysis Fluorescence Recovery After Photobleaching Funding Goals Health Histology In Situ Intervention Joints Knock-out Lead Liver Acinus Zone 3 Measurement Measures Mechanical Stress Mechanics Mediating Metabolic Microscopy Modeling Molecular Mus Operative Surgical Procedures Osteoarthrosis Deformans Pathway interactions Pharmacologic Substance Play Property Proteoglycan Rate Regulation Role Signal Transduction Site Staging Stress Structure Techniques Testing Theoretical model Tissues Transgenic Mice Transport Process articular cartilage cartilage metabolism improved macromolecule novel physical property research study response size wasting

项目摘要

The mechanical environment of the chondrocytes is an important factor that affects the health and function of the diarthrodial joint. The mechanical signals to which chondrocytes are exposed depend on the biomechanical interactions between the cell, pericellular matrix, and extracellular matrix. Currently, there is little or no information available on the mechanical properties of the pericellular matrix of articular cartilage. The goals of this study are to measure the intrinsic biomechanical and diffusion properties of the chondrocyte pericellular matrix, and to test the hypothesis that these properties are altered in osteoarthritic cartilage. Furthermore, we propose that type VI collagen, which is abundantly present in the pericellular matrix, influences the physical properties of this region. We will use several novel experimental techniques to quantify the micromechanical behavior of pericellular matrix using the isolated _hondron model. The specific aims of this study are: 1) Measure the mechanical properties of the 3ericellular matrix from normal and osteoarthritic cartilage using micropipette aspiration and atomic force microscopy, incorporate these findings in a theoretical model of cell-matrix interactions in cartilage, and validate these predictions using 3D confocal microscopy; 2) Measure the diffusion properties of the pericellular matrix of normal and OA cartilage; 3) Determine how the presence of a normal or OA pericellular matrix influences the metabolic response of chondrocytes to dynamic compression within an lartificial matrix; and 4) Determine what role type VI collagen plays in the mechanical properties of the pericellular matrix. The long-term goals of this study are to improve our understanding of the role of mechanical factors in the regulation of cartilage metabolism in normal and diseased conditions. A better understanding of these pathways will hopefully lead to the development of new pharmaceutical or biophysical interventions for the treatment of osteoarthritis.

软骨细胞的力学环境是影响其健康和健康的重要因素。腹股沟关节的功能。软骨细胞所暴露的机械信号取决于细胞、细胞周围基质和细胞外基质之间的生物力学相互作用。目前，有关关节细胞周围基质的力学性质的信息很少或根本没有。软骨。本研究的目的是测量内固定材料的内在生物力学和扩散特性。软骨细胞细胞周围基质，并检验这些特性在骨关节炎软骨。此外，我们提出了VI型胶原，它丰富地存在于细胞周围基质，影响这一区域的物理性质。我们将用几本小说细胞外基质微观力学行为量化的实验技术 _洪德龙模型。本研究的具体目的是：1)测量材料的力学性能 3用微管抽吸法和原子力法从正常软骨和骨关节病软骨中提取细胞基质显微镜下，将这些发现纳入软骨细胞-基质相互作用的理论模型中，并且使用3D共聚焦显微镜验证这些预测；2)测量正常软骨和骨性关节炎的细胞外基质；3)确定正常或骨性关节炎的存在细胞外基质影响软骨细胞对动态压缩的代谢反应以及4)确定VI型胶原在血管内皮细胞的力学性能中所起的作用细胞周围基质。这项研究的长期目标是提高我们对在正常和疾病条件下软骨代谢调节中的力学因素。更好的对这些途径的理解将有望导致新的药物或治疗骨性关节炎的生物物理干预。