Small Animal Laboratory Core

这个项目的主题是以活体临床前应用为基础的 研究癌症对骨骼影响的分子机制的模型。 每个项目都需要使用动物模型，并对其进行仔细的评估和监测 用于骨骼和软组织转移的动物。这里提出的小动物核心将 为每个单独项目中涉及动物的所有工作提供支持。服务将包括 维持肿瘤细胞系，管理所有涉及小动物的程序以及 评估癌症对骨骼影响的最新方法。这一核心构建在 通过采用光学成像等新技术，实现了以前存在的实验室岩心 表达绿色荧光蛋白的肿瘤细胞、microSPECT、microPET和microCT 评估肿瘤对骨骼和肿瘤负担的影响。小的一个额外的力量 动物核心是这些领域公认的专家的参与。技术，如 MicroSPECT、Micro PET和MicroCT在双侧乳腺临床诊断中的应用 癌症和多发性骨髓瘤，但据我们所知，还没有在小动物身上使用 转移性疾病的模型。使用这些技术将使我们能够进行直接比较 我们的临床前模型和患者的疾病之间的关系，为我们的临床前模型提供了验证 乳腺癌和多发性骨髓瘤的模型。此外，这些技术将建立在这些基础上 我们在过去使用的，如放射摄影术和组织形态计量学，通过允许我们获得 更全面的肿瘤负荷和在整个完整动物中的分布情况，而 提供更好的分辨率。

The theme of this Program Project Grant is heavily based around the use of preclinical in vivo models to study the molecular mechanisms involved with the effects to cancer on the skeleton. Each project requires the use of animal models and careful assessment and monitoring of those animals for both skeletal and soft tissue metastases. The Small Animal Core proposed here will provide support for all work involving animals within each individual project. Services will include maintenance of tumor cell lines, management of all procedures involving small animals as well as state-of-the art methods for assessing the effects of cancer on the skeleton. This core builds on the previously existing laboratory core by incorporating new techniques such as optical imaging of green fluorescent protein expressing tumor cells, MicroSPECT, MicroPET and microCT for the assessment of tumor effects of the skeleton and tumor burden. An additional strength of the Small Animal Core is the participation of recognized experts in these areas. Techniques such as MicroSPECT, Micro PET and MicroCT are used in clinical diagnostics for patients with both breast cancer and multiple myeloma but to our knowledge have not yet been utilized in small animal models of metastatic disease. Use of these techniques will allow us to make direct comparisons between our preclinical models and disease in patients providing for validation of our preclinical models of breast cancer and multiple myeloma. Furthermore, these techniques will build on those that we have used in the past such as radiography and histomorphometry by allowing us to obtain a much more thorough picture of tumor burden and distribution in the whole intact animal, while providing much better resolution.