Regulation of virulence factos in Bartonella henselae
Regulation of virulence factos in Bartonella henselae
批准号:
7433611
负责人:
BURT E ANDERSON
金额:
$32.63万
依托单位国家:
美国
项目类别:
财政年份:
1995
资助国家:
美国
项目状态:
已结题
起止时间:
1995-07-01 至 2008-07-31
关键词:
Angiogenic FactorAntigensBacillary AngiomatosisBacteriaBacterial AdhesinsBacterial GenesBacterial TypingBartonellaBartonella henselaeBindingBiological AssayBlood VesselsCat-Scratch DiseaseCellsClinicalDNADNA Microarray ChipDNA Microarray formatDNA deliveryDataDiseaseEffector CellEndothelial CellsEnvironmentFutureGene ExpressionGene Expression RegulationGenesGenetic TranscriptionGoalsImmunocompromised HostIndividualInfectionInfectious AgentLaboratoriesLesionLifeMediatingModelingOperonOrganPathogenesisPatientsPeptidesPhenotypePlatelet Factor 4PlayProcessProductionProtein OverexpressionProteinsRangeRecruitment ActivityRegulationRegulator GenesReportingRoleSignal TransductionSkinSurfaceSyndromeSystemSystemic infectionTestingTranscriptional ActivationType IV Secretion System PathwayUp-RegulationVascular Endothelial Growth FactorsVirulenceVirulence FactorsVisceralangiogenesisantimicrobialbasegene therapygenetic regulatory proteinin vivoknockout genepromoter
中文摘要
汉赛巴尔通体(Bartonella henselae)细菌引起多种疾病综合征,包括严重的全身性
在某些患者中,特别是在免疫功能低下的个体中,其中的一个表现
新出现的感染因子是杆菌性血管瘤病,其特征在于存在血管性血管瘤。
感染患者皮肤和内脏器官的增生性病变。本项目的目标是测试
中心假设B.汉赛线虫利用几种毒力因子基因的协调调节,
增强其促进血管生成的能力。至少有两个重要的毒力因子已被确定,
B。在引起血管生成中起作用的Henselae。第一个是virB操纵子,它编码IV型
分泌系统,其负责递送作用于内皮细胞以促进内皮细胞增殖的效应蛋白。
延长生存期。第二个是位于B表面的主要粘附素BadA。汉塞莱和哈斯
已显示在感染细胞中诱导血管内皮生长因子分泌中是重要的。我们
初步数据表明,B. henselae至少在
部分负责这些基因的调控。为检验这一假设,提出了以下具体目标:
1)确定ompR和envZ表达与virB操纵子表达之间的相关性,2)
表征参与ompR/envZ上调virB的机制,以及3)鉴定其他毒力
ompR/envZ控制下的因子基因。这些研究应该帮助我们理解这是如何以及为什么的。
细菌在一些患者中引起轻微疾病,在另一些患者中引起危及生命的感染,
血管生成性病变此外,控制细菌的基因调控系统的表征,
毒力因子可能被证明是抗微生物治疗的有价值的靶点。这一应用产品
项目是描述virB IV型分泌系统基因的调控机制。等
这些信息可能在未来尝试利用这种分泌系统来递送
DNA和蛋白质靶向细胞进行基因治疗。
英文摘要
The bacterium Bartonella henselae causes a variety of disease syndromes including severe systemic
infections in some patients, particularly in immunocompromised individuals. One manifestation of this
emerging infectious agent is bacillary angiomatosis which is characterized by the presence of vascular
proliferative lesions of the skin and visceral organs in infected patients. The goal of this project is to test the
central hypothesis that B. henselae utilizes coordinate regulation of several virulence factor genes to
enhance it's ability to promote angiogenesis. At least two important virulence factors have been identified in
B. henselae that play a role in causing angiogenesis. The first is the virB operon that encodes a type IV
secretion system that is resposible for delivery of the effector proteins that act on endothelial cells to promote
their extended survival. The second is the major adhesin BadA that is on the surface of B. henselae and has
been shown to be important in inducing vascular endothelial growth factor secretion in infected cells. Our
preliminary data suggest that the two-component regulatory sytem OmpR/EnvZ of B. henselae is at least in
part resposible for regulation of these genes. The following specific aims are proposed to test the hypothesis;
1) define the correlation between ompR and envZ expression and expression of the virB operon, 2)
characterize the mechanism involved in ompR/envZ upregulation of virB, and 3) identify other virulence
factor genes under control of ompR/envZ. These studies should help us understand how and why this
bacterium causes mild disease in some patients and life-threatening infections in other pateints resulting in
angiogenic lesions. Furthermore, characterization of a gene regulatory system that controls bacterial
virulence factors may prove to be a valuable target for antimicrobial therapy. An applied product of this
project is the description of the regulatory mechanism of the virB type IV secretion system genes. Such
information may prove valuable in future attempts to harness the use of this secretion system for delivery of
DNA and protein to target cells for gene therapy.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
A novel zebrafish embryo model to define virulence factors of Bartonella henselae
-
批准号:8911767
-
项目类别:
-
资助金额:$18.69万
-
财政年份:2014
-
负责人:BURT E ANDERSON
-
依托单位:
A novel zebrafish embryo model to define virulence factors of Bartonella henselae
-
批准号:8651984
-
项目类别:
-
资助金额:$22.43万
-
财政年份:2014
-
负责人:BURT E ANDERSON
-
依托单位:
Regulation of virulence factors in Bartonella henselae
-
批准号:8111405
-
项目类别:
-
资助金额:$14.55万
-
财政年份:2010
-
负责人:BURT E ANDERSON
-
依托单位:
Regulation of virulence factors in Bartonella henselae
-
批准号:7665116
-
项目类别:
-
资助金额:$33.08万
-
财政年份:2008
-
负责人:BURT E ANDERSON
-
依托单位:
Regulation of virulence factors in Bartonella henselae
-
批准号:7907681
-
项目类别:
-
资助金额:$32.74万
-
财政年份:2008
-
负责人:BURT E ANDERSON
-
依托单位:
Regulation of virulence factors in Bartonella henselae
-
批准号:7523995
-
项目类别:
-
资助金额:$33.08万
-
财政年份:2008
-
负责人:BURT E ANDERSON
-
依托单位:
Regulation of virulence factors in Bartonella henselae
-
批准号:8097990
-
项目类别:
-
资助金额:$32.42万
-
财政年份:2008
-
负责人:BURT E ANDERSON
-
依托单位:
MOLECULAR CHARACTERIZATION OF ROCHALIMAEA ANTIGENS
-
批准号:2672522
-
项目类别:
-
资助金额:$10.13万
-
财政年份:1995
-
负责人:BURT E ANDERSON
-
依托单位:
MOLECULAR CHARACTERIZATION OF ROCHALIMAEA ANTIGENS
-
批准号:2075119
-
项目类别:
-
资助金额:$9.39万
-
财政年份:1995
-
负责人:BURT E ANDERSON
-
依托单位:
Bartonella henselae antigens of biological significance
-
批准号:6627792
-
项目类别:
-
资助金额:$32.63万
-
财政年份:1995
-
负责人:BURT E ANDERSON
-
依托单位:
Bartonella henselae antigens of biological significance
-
批准号:6868099
-
项目类别:
-
资助金额:$32.63万
-
财政年份:1995
-
负责人:BURT E ANDERSON
-
依托单位:
MOLECULAR CHARACTERIZATION OF ROCHALIMAEA ANTIGENS
-
批准号:2887028
-
项目类别:
-
资助金额:$10.52万
-
财政年份:1995
-
负责人:BURT E ANDERSON
-
依托单位:
Bartonella henselae antigens of biological significance
-
批准号:6495918
-
项目类别:
-
资助金额:$32.15万
-
财政年份:1995
-
负责人:BURT E ANDERSON
-
依托单位:
MOLECULAR CHARACTERIZATION OF ROCHALIMAEA ANTIGENS
-
批准号:2442652
-
项目类别:
-
资助金额:$9.75万
-
财政年份:1995
-
负责人:BURT E ANDERSON
-
依托单位:
Bartonella henselae antigens of biological significance
-
批准号:6703078
-
项目类别:
-
资助金额:$32.63万
-
财政年份:1995
-
负责人:BURT E ANDERSON
-
依托单位:
MOLECULAR CHARACTERIZATION OF ROCHALIMAEA ANTIGENS
-
批准号:2075118
-
项目类别:
-
资助金额:$10.16万
-
财政年份:1995
-
负责人:BURT E ANDERSON
-
依托单位:
国内基金
海外基金
Neo-antigens暴露对肾移植术后体液性排斥反应的影响及其机制研究
-
批准号:2022J011295
-
项目类别:省市级项目
-
资助金额:10.0万元
-
批准年份:2022
-
负责人:王亚伟
-
依托单位:
结核分枝杆菌持续感染期抗原(latency antigens)的重组BCG疫苗研究
-
批准号:30801055
-
项目类别:青年科学基金项目
-
资助金额:19.0万元
-
批准年份:2008
-
负责人:王丽梅
-
依托单位: