Transgenic/Knockout Mice

转基因/基因敲除小鼠

• 批准号: 7081710
• 负责人: ASTAR WINOTO
• 金额: $ 23.68万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-15 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7081710
• 关键词: T cell receptor; animal care; animal colony; biomedical facility; biotechnology; cellular immunity; disease /disorder model; gene targeting; genetic polymorphism; genetic promoter element; genetic strain; genetically modified animals; green fluorescent proteins; host organism interaction; immunogenetics; immunoregulation; laboratory mouse; major histocompatibility complex; microorganism immunology; parasite infection mechanism; virus infection mechanism

The ability to manipulate the mouse genome through transgenic and knock-out or knock-in technologies has revolutionized modern Immunology. The purpose of this core facility (Core B) is to integrate the transgenic and gene targeting approach into the studies of host-pathogen interaction, a major thrust of the program project "Mouse models for understanding host-pathogen interactions". This Transgenic/Knockout Mouse Core will provide the critical technical capability to generate and to maintain transgenic and mutant mice commonly used by two or more investigators of the program project (PPG). In particular, this Core will complement Core C to provide transgenic mice expressing fluorescent proteins to be used for imaging studies. Core B will also generate and distribute transgenic strains of mice as needed by the individual investigator in the PPG. In aim 1, C57Bl/6 transgenic mice expressing fluorescent genes, whose expression is driven by different promoter/enhancers will be generated and maintained for use in video imaging experiments. These include mice expressing membrane Cyan Fluorescent Protein (CFP) in dendritic cells or macrophages and mice with CFP or YFP (Yellow Fluorescent Protein) expressed in all cell types. In aim 2, knock-out/knock-in mice with genes involved in innate immunity will be maintained as well as back-crossed to C57BI/6 or Balb/c if necessary. These mice, including DC- and macrophage-specific FADD-/-, MyD88-/-, IFN-alpha/betaR-/-, TRAIL-R-/-, NK deficient mice, NKG2D-/-, IL-12/GFP (Green Fluorescent Protein) knock-in and lFN-gamma/GFP knock-in mice, will be provided to investigators in the PPG for their biochemical, infection or imaging experiments. In aim 3, Core B will generate and provide transgenic mice for the specific needs of the individual PPG investigator. Completion of these aims is necessary and essential for the success of the PPG designed to understand how the immune system interacts with model pathogens.

通过转基因和敲除或敲入技术操纵小鼠基因组的能力 彻底改变了现代免疫学此核心设施（核心B）的目的是整合 转基因和基因打靶方法进入宿主-病原体相互作用的研究， “理解宿主-病原体相互作用的小鼠模型”项目。这 转基因/敲除小鼠核心将提供关键的技术能力， 转基因和突变小鼠通常由两个或两个以上的研究人员的计划项目（PPG）。 特别是，这个Core将补充Core C，以提供表达荧光蛋白的转基因小鼠 用于成像研究。核心B还将产生和分发转基因小鼠品系， 个人研究者在PPG中需要的。目的1：建立C57 B1/6转基因小鼠， 将产生荧光基因，其表达由不同的启动子/增强子驱动， 保持用于视频成像实验。这些包括表达膜青色的小鼠， 树突状细胞或巨噬细胞和CFP或YFP小鼠中的荧光蛋白（CFP）（黄色） 荧光蛋白）在所有细胞类型中表达。在aim 2中，基因敲除/敲入小鼠 在先天免疫中，如果需要的话，将维持并回交到C57 BI/6或Balb/c。这些 小鼠，包括DC-和巨噬细胞特异性FADD-/-、MyD 88-/-、IFN-α/β R-/-、TRAIL-R-/-、NK缺陷型 小鼠，NKG 2D-/-、IL-12/GFP（绿色荧光蛋白）敲入和IFN-γ/GFP敲入小鼠，将被 提供给PPG中的研究者用于其生化、感染或成像实验。在目标3中， 核心B将生成并提供转基因小鼠，以满足个体PPG的特定需求 调查员这些目标的完成是PPG成功的必要和关键， 了解免疫系统如何与模型病原体相互作用。