Antidiabetic Constituents from the Dominican Medicinal Plant Momordica charantia

多米尼加药用植物苦瓜的抗糖尿病成分

• 批准号: 7409263
• 负责人: Amy Celeste Keller
• 金额: $ 2.77万
• 依托单位: HERBERT H. LEHMAN COLLEGE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7409263
• 关键词: Affect; Antidiabetic Drugs; Biological Assay; Blood Glucose; Cell Line; Cells; Chemical Structure; Chronic Disease; Collection; Communities; Complementary and alternative medicine; Cucurbitaceae; Diabetes Mellitus; Diagnosis; Disease; Dominican; Fruit; Goals; Herbal Medicine; Hispanics; Human; Hyperglycemia; Hypoglycemia; Hypoglycemic Agents; In Vitro; Insulin; Insulin-Dependent Diabetes Mellitus; Investigation; Islet Cell; Islets of Langerhans; Juice; Left; Measures; Medical; Medicinal Plants; Medicine; Metformin; Methods; Modeling; Momordica charantia; Morbidity - disease rate; Non-Insulin-Dependent Diabetes Mellitus; Not Hispanic or Latino; Obesity; Oral; Pharmaceutical Preparations; Phytochemical; Pioglitazone; Plants; Plasma; Population; Rattus; Research; Research Technics; Rodent Model; Standards of Weights and Measures; Techniques; Testing; Training; United States; Week; Whole Blood; base; career; cell growth; diabetic; follow-up; improved; in vitro Assay; in vivo; insulin secretion; mortality; mouse model; skills; stem

DESCRIPTION (provided by applicant): Momordica charantia is a widely-used plant in traditional Dominican medicine for diabetes treatment. This project hypothesizes that the active compounds isolated from M. charantia fruits lower blood glucose concentrations via an increase in (3-cell replication and function, resulting in improved insulin secretion. To test this hypothesis, the fruits of M. charantia will be extracted, fractionated, and tested with a p-cell assay in order to identify the active fractions. Various chromatographic methods will be used to isolate the bioactive compounds from the active fractions. After isolation, the active compounds will be identified using modern phytochemical techniques and further tested in vitro using (3-cells to quantify the effects against an established oral hypoglycemic drug as a reference. Two of the bioactive compounds will be selected for in vivo testing based on their activity in the (3-cell line, uniqueness of chemical structure, and yield. The selected compounds will then be tested in a mouse model of obesity-induced hyperglycemia along with positive controls of commonly prescribed medications for Type II diabetes mellitus to functionally investigate the compounds' hypoglycemic activity. In addition, the active compounds isolated from the fruit will be quantified in the stems and leaves of M. charantia, as these parts of the plants are used in traditional Dominican medicine. Many traditional remedies have not been rigorously researched in the scientific and medical communities. Therefore, this project will provide training both in phytochemical techniques and research, and also in the skills necessary for the scientific research of herbal medicine. This interdisciplinary training is vital to a goal of a lifetime career in the rigorous scientific study of complementary and alternative medicine. Momordica charantia is a widely-used plant in treating one of the world's most threatening diseases, diabetes. Although this plant's antidiabetic activity has been researched, very few studies have rigorously tested the bioactive M. charantia compounds for specific mechanisms of action. The results of this study will allow people to make more informed decisions about the use of M. charantia in diabetes treatment.

描述（申请人提供）：苦瓜（Momordica charantia）是多米尼加传统医学中广泛用于治疗糖尿病的植物。本项目假设，从沙兰果实中分离的活性化合物通过增加3细胞的复制和功能来降低血糖浓度，从而改善胰岛素分泌。为了验证这一假设，我们将提取沙兰的果实，对其进行分离，并用p细胞测定法进行测试，以确定其活性组分。将使用各种色谱方法从活性组分中分离出生物活性化合物。分离后，将使用现代植物化学技术鉴定活性化合物，并使用3-细胞在体外进行进一步测试，以量化对既定口服降糖药的影响，作为参考。其中两种生物活性化合物将根据其在3细胞系中的活性、化学结构的独特性和产量进行体内试验。然后，选定的化合物将在肥胖引起的高血糖小鼠模型中进行测试，同时对II型糖尿病常用处方药的阳性对照进行测试，以功能性地研究化合物的降糖活性。此外，从果实中分离出的活性化合物将在M. charantia的茎和叶中进行量化，因为这些植物的这些部分被用于传统的多米尼加医学。许多传统疗法在科学界和医学界都没有经过严格的研究。因此，该项目将提供植物化学技术和研究方面的培训，以及草药科学研究所需的技能。这种跨学科的培训对于在补充和替代医学的严格科学研究中终身职业生涯的目标至关重要。苦瓜（Momordica charantia）是一种广泛用于治疗世界上最具威胁性的疾病之一——糖尿病的植物。虽然这种植物的抗糖尿病活性已经被研究过，但很少有研究严格地测试了这种生物活性化合物的具体作用机制。这项研究的结果将使人们对在糖尿病治疗中使用M. charantia做出更明智的决定。