NIH DIRECTOR'S PIONEER AWARD
美国国立卫生研究院院长先锋奖
基本信息
- 批准号:7292761
- 负责人:
- 金额:$ 78.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-09-28 至 2011-07-31
- 项目状态:已结题
- 来源:
- 关键词:AdoptedAmino Acid SequenceAnabolismAwardBacterial ProteinsCellsCollaborationsComplexConditionDefectDiseaseEukaryotic CellFluorescenceIn VitroLabelLeadMethodsModelingNuclear Magnetic ResonanceOrganismPatternProcessProteinsReactionRelianceReportingResearchSignal TransductionStructureSystemTestingTherapeutic InterventionTubecellular imagingfitnessin vivointerestnovelprotein expressionprotein foldingprotein function
项目摘要
Surprisingly little is known about how proteins fold in vivo, yet it is this process, and not the test-tube
idealized folding reaction so intensively studied over the past several decades, that is crucial to the
fitness of an organism. The fidelity of folding and the stability of proteins in the cell are critical to their
functions, their degradation, and their vulnerability to aggregation. Many diseases are now known to
arise from defects in protein folding, either because of the loss or alteration of essential protein
functions, or because of the build-up of toxic species such as aggregates. We believe that novel
methods and creative collaborations will allow us to overcome the daunting technical obstacles that
have impeded progress on protein folding in the cell. Focusing first on a small model protein for which
we have detailed descriptions of folding in vitro will enable methods optimization. Folding in cellular
conditions will be followed in systems of increasing complexity: bacterial protein expression, cell-free
biosynthesis, and semi-permeabilized or intact eukaryotic cell expression. The new strategies will
reveal how larger proteins of biomedical interest adopt their structures in their cellular context and how
this process may go awry. Methodologically, we anticipate placing major reliance on spectroscopic
methods, including fluorescence and nuclear magnetic resonance, and using novel labeling strategies
to observe the protein under study in the complex cellular milieu. Complementary in-cell imaging
methods will be used to insure that observed signals report on relevant phenomena and to reveal novel
functionally significant spatial localization patterns. We anticipate that this research will lead to new
paradigms for how amino acid sequences encode folding information and that the resulting enhanced
understanding of folding in vivo will lead to new strategies for therapeutic intervention in misfolding and
aggregation diseases.
令人惊讶的是,我们对蛋白质如何在体内折叠知之甚少,但它是这个过程,而不是试管
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
LILA M GIERASCH其他文献
LILA M GIERASCH的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('LILA M GIERASCH', 18)}}的其他基金
Protein folding in the cell: Challenges and coping mechanisms
细胞中的蛋白质折叠:挑战和应对机制
- 批准号:
10410352 - 财政年份:2016
- 资助金额:
$ 78.5万 - 项目类别:
Protein folding in the cell: Challenges and coping mechanisms
细胞中的蛋白质折叠:挑战和应对机制
- 批准号:
10808021 - 财政年份:2016
- 资助金额:
$ 78.5万 - 项目类别:
Protein folding in the cell: Challenges and coping mechanisms
细胞中的蛋白质折叠:挑战和应对机制
- 批准号:
10647692 - 财政年份:2016
- 资助金额:
$ 78.5万 - 项目类别:
Protein folding in the cell: Challenges and coping mechanisms Administrative Supplement for Equipment Purchase
细胞内蛋白质折叠:挑战与应对机制设备采购行政补充
- 批准号:
10795171 - 财政年份:2016
- 资助金额:
$ 78.5万 - 项目类别:
Administrative Supplement to Protein folding in the cell: Challenges and coping mechanisms
细胞内蛋白质折叠的行政补充:挑战和应对机制
- 批准号:
10592508 - 财政年份:2016
- 资助金额:
$ 78.5万 - 项目类别:
Allosteric Mechanism of Hsp70 Molecular Chaperones
Hsp70分子伴侣的变构机制
- 批准号:
7924926 - 财政年份:2009
- 资助金额:
$ 78.5万 - 项目类别:
相似海外基金
Cerebral infarction treatment strategy using collagen-like "triple helix peptide" containing functional amino acid sequence
含功能氨基酸序列的类胶原“三螺旋肽”治疗脑梗塞策略
- 批准号:
23K06972 - 财政年份:2023
- 资助金额:
$ 78.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Establishment of a screening method for functional microproteins independent of amino acid sequence conservation
不依赖氨基酸序列保守性的功能性微生物蛋白筛选方法的建立
- 批准号:
23KJ0939 - 财政年份:2023
- 资助金额:
$ 78.5万 - 项目类别:
Grant-in-Aid for JSPS Fellows
Effects of amino acid sequence and lipids on the structure and self-association of transmembrane helices
氨基酸序列和脂质对跨膜螺旋结构和自缔合的影响
- 批准号:
19K07013 - 财政年份:2019
- 资助金额:
$ 78.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Construction of electron-transfer amino acid sequence probe with an interaction for protein and cell
蛋白质与细胞相互作用的电子转移氨基酸序列探针的构建
- 批准号:
16K05820 - 财政年份:2016
- 资助金额:
$ 78.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of artificial antibody of anti-bitter taste receptor using random amino acid sequence library
利用随机氨基酸序列库开发抗苦味受体人工抗体
- 批准号:
16K08426 - 财政年份:2016
- 资助金额:
$ 78.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The aa15-17 amino acid sequence in the terminal protein domain of HBV polymerase as a viral factor affect-ing in vivo as well as in vitro replication activity of the virus.
HBV聚合酶末端蛋白结构域中的aa15-17氨基酸序列作为影响病毒体内和体外复制活性的病毒因子。
- 批准号:
25461010 - 财政年份:2013
- 资助金额:
$ 78.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Amino acid sequence analysis of fossil proteins using mass spectrometry
使用质谱法分析化石蛋白质的氨基酸序列
- 批准号:
23654177 - 财政年份:2011
- 资助金额:
$ 78.5万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Precise hybrid synthesis of glycoprotein through amino acid sequence-specific introduction of oligosaccharide followed by enzymatic transglycosylation reaction
通过氨基酸序列特异性引入寡糖,然后进行酶促糖基转移反应,精确杂合合成糖蛋白
- 批准号:
22550105 - 财政年份:2010
- 资助金额:
$ 78.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Estimating selection on amino-acid sequence polymorphisms in Drosophila
果蝇氨基酸序列多态性选择的估计
- 批准号:
NE/D00232X/1 - 财政年份:2006
- 资助金额:
$ 78.5万 - 项目类别:
Research Grant
Construction of a neural network for detecting novel domains from amino acid sequence information only
构建仅从氨基酸序列信息检测新结构域的神经网络
- 批准号:
16500189 - 财政年份:2004
- 资助金额:
$ 78.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)