The Effects of Microglia on Oligodendrocyte Cell Death

小胶质细胞对少突胶质细胞死亡的影响

• 批准号: 7495740
• 负责人: BRANDON A MILLER
• 金额: $ 4.55万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-15 至 2009-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7495740
• 关键词: Accounting; Actins; Address; Affect; Agonist; Asses; Brain Hypoxia-Ischemia; Cell Communication; Cell Death; Cells; Central Nervous System Diseases; Cessation of life; Coculture Techniques; Condition; Development; Disease; Environment; Experimental Models; Exposure to; Free Radicals; Future; Generations; Glutamate Receptor; Glutamates; Immunohistochemistry; In Vitro; Incubators; Infection; Injury; Kainic Acid; Laboratories; Lipopolysaccharides; Measures; Mediating; Mediator of activation protein; Microglia; Modeling; Neuraxis; Neurotransmitters; Oligodendroglia; Oligonucleotides; Oxygen; Pathway interactions; Physical condensation; Poison; Predisposing Factor; Predisposition; Process; Production; Reporting; Source; Staging; Toxic effect; Trauma; Tumor Necrosis Factor-alpha; cytokine; day; design; extracellular; human disease; insight; research study; response

DESCRIPTION (provided by applicant): Oligodendrocyte (oligo) cell death occurs in response to several pathophysiological processes, including ischemia, hypoxia, infection and trauma. Several laboratories have shown that oligos in the above models of injury die or are damaged by excessive amounts of the neurotransmitter glutamate. However, there are varying reports of just how susceptible oligos are to glutamate, and it is not known what factors are responsible these differing results. The proposed studies will begin to determine how the extracellular environment in a damaged central nervous system (CNS) changes oligos' response to glutamate. This proposal will focus on how two factors - oxygen changes and the presence of microglia - affect oligos vulnerability to toxic substances released during CNS trauma and disease. Using Oligodendrocyte and microglia cultures, both in isolation and combination, along with oxygen-regulating incubators and the glutamate receptor agonist kainic acid, the proposed experiments will help determine what conditions and cellular responses in the CNS mediate oligo death, and provide insights into future therapies to protect oligos in multiple diseases.

描述（由申请人提供）：