Development of A Lab-on-a-Chip System for Saliva-Based Diagnostics

开发基于唾液的诊断芯片实验室系统

• 批准号: 7477446
• 负责人: JOHN T MCDEVITT
• 金额: $ 28.63万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7477446
• 关键词: Address; Agreement; Anus; Area; Arts; Bacillus (bacterium); Biological Assay; Biological Markers; Biosensor; Buffers; C-reactive protein; CD40 Ligand; Cardiac; Cardiovascular system; Cells; Characteristics; Chemicals; Chemistry; Chronic; Class; Clinical; Communication; Complex; Computer software; Condition; Coronary Arteriosclerosis; Cytometry; Data; Data Analyses; Detection; Development; Devices; Diagnosis; Diagnostic; Diagnostic tests; Disease; Doctor of Medicine; Dose; Edentulous Mouth; Electrodes; Electronics; Elements; Engineering; Enzyme-Linked Immunosorbent Assay; Equipment; Event; Fingerprint; Fluorescence; Foundations; Funding; Future; General Hospitals; Generations; Glass; Goals; Gold; HIV; Hand; Health Status; Healthcare; Human; Immunoassay; Individual; Industry; Inflammation; Inflammatory; Injection of therapeutic agent; Interleukin-6; Invasive; Joints; Laboratories; Liquid substance; Marriage; Massachusetts; Measurement; Measures; Methodology; Methods; Metric; Microfabrication; Microfluidic Microchips; Microfluidics; Mission; Molds; Monitor; Nanotechnology; Neutrophil Collagenase; None or Not Applicable; Operative Surgical Procedures; Optics; Oral; Oral health; Parotid Gland; Patients; Pattern; Performance; Periodontal Diseases; Periodontitis; Physiological; Plastics; Polymerase Chain Reaction; Polymers; Principal Investigator; Procedures; Process; Proteins; Proteomics; Range; Recovery; Reporting; Reproduction spores; Research; Research Infrastructure; Research Personnel; Resources; Risk; Risk Assessment; Saliva; Salivary; Sampling; Science; Series; Serum; Source; Staging; Standards of Weights and Measures; Structure; System; Systemic disease; Systems Analysis; Technology; Testing; Texas; Thromboplastin; Time; Translating; Treatment outcome; Tumor Necrosis Factor-alpha; Tumor Necrosis Factors; Tumor necrosis factor receptor 11b; United States Food and Drug Administration; United States National Institutes of Health; Universities; Validation; Walkers; Wireless Technology; Work; austin; base; biochip; concept; data acquisition; design; human TNF protein; human disease; immune function; instrument; instrumentation; lens; medical schools; micro-total analysis system; miniaturize; oral fluid diagnostic; point of care; pre-clinical; programs; prototype; real world application; response; saliva diagnostic; salivary diagnostics; sensor; success; tool

Saliva is an important diagnostic fluid that has the potential to be used for detection of essential biomarkers for both oral and systemic diseases. Unfortunately, the current lack of reliable detection methods for measurement of important analytes within this complex biomatrix has hindered progress in salivary diagnostics. To help remove this barrier, recent promising activities have been initiated in microfluidic-based Lab-on-a-Chip (LOG) systems. While several miniaturized platforms are being developed now, few of these systems have evolved to the point where important protein biomarkers can be measured over the physiological range using real saliva samples. Towards this essential goal, the team of investigators from UT and UK have developed and documented carefully an integrated biochip assay system that is suitable for measurement of panels of important inflammatory markers (e.g.CRP, IL-1beta, MMP-8, TNF-alpha, IL-6)in saliva. This newly fashioned ultra-sensitive method has been used to extend saliva-based diagnostics to the low levels of CRP found in saliva, therefore allowing for the first comparison of CRP levels between healthy, periodontitis, and edentulous patients. The miniaturized system correlates nicely with established macroscopic "gold standard" methods. Thus, it now important to take the next steps to combine these functional components and associated methodologies so as to create a flexible platform system that may be used to promote and expand the field of salivary diagnostics. This U01 renewal program targets the development of portable devices that can be used to complete salivary diagnostic tests. The program will evolve with important biochip, analyzer and immunoassay development milestones to be completed in the first two years of the program. In year 1, integrated biochips and analyzers will be developed and tested. These alpha prototypes will be used to define specifications for more final systems. In year 2, fully developed biochips and analyzer suitable to serve, as beta prototypes will be fashioned. These highly functional portable systems will be used in years 3-5 to complete pre-clinical testing and validation. Salivary biomarker fingerprints that our team has already defined will be used to 1) discriminate stages of periodontitis and 2) identify risk for future cardiac events in coronary artery disease patients. Final data acquisition and FDA approval will be completed by year 5.

唾液是一种重要的诊断液体，具有用于检测基本生物标志物的潜力