Genetic Epidemiology

遗传流行病学

• 批准号: 7288861
• 负责人: ALISA GOLDSTEIN
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7288861
• 关键词: Genetic; Epidemiology

Many of the investigations in this genetic epidemiology project arise from observations in families at high risk of cancer or in other etiologic studies. Case-control study data from 718 non-Hispanic white patients with invasive cutaneous melanoma from melanoma clinics in Philadelphia and San Francisco and 945 matched controls from outpatient clinics with similar catchment areas to the cases are being used to develop a risk assessment model for estimating absolute risk of melanoma. Previously, DNA repair capacity (DRC) in lymphocytes from subjects from a case-control study of 183 incident melanoma cases and 179 controls conducted in North-Eastern Italy showed that DRC did not differ between cases and controls overall, but subjects with low DRC and poor tanning ability or dysplastic nevi were at increased risk of melanoma. To explore the genetic basis of these findings, a series of SNPs in DNA repair genes were analyzed. SNPs found in DNA polymerase or abasic repair genes were associated with reduced melanoma risk, while SNPs in NER genes were associated with higher risk in older subjects, or with decreased DRC. Standardized instruments were used to measure subjects constitutive skin color and UV sensitivity, and the role of SNPs was analyzed in potential pigmentation genes. The results showed that both colorimeter-based skin brightness and variants of the MC1R pigmentation gene were associated with melanoma risk, and the risk increased with the number of MC1R variants, even after adjustment for the traditionally assessed pigmentation characteristics. MC1R variants were also associated with melanoma thickness in the cases. As a follow-up to a DCEG comprehensive case-control study of adults with brain tumors, a family-based study of the parents, siblings and adult children of the 480 eligible glioma cases has been conducted. Relatives of 365 of the glioma cases were interviewed about personal and family medical history and other risk factors and were asked to provide buccal cells as a source of DNA. Analyses to examine the risk of cancer among the first degree relatives compared to population controls are in process. In the future, the relatives will be used as controls for the glioma cases in association studies and in analyses to evaluate the roles of genetic susceptibility and environmental exposures on the risk of gliomas and etiologically related tumors. Retrospective review of clinical data comparing 33 medulloblastoma patients from a single institution to their 46 unaffected relatives revealed a paucity of clinical findings among the majority of medulloblastoma patients. The results suggest that clinically recognizable syndromes are uncommon among patients with medulloblastoma, however, PTCH1 and SUFU mutations were present at a low but significant frequency.Registry data from Sweden and Denmark are continuing to be analyzed. A paper in press describes the risk of lymphoproliferative (LP) tumors in relatives of patients with non-Hodgkin lymphoma (NHL). Relatives were at significantly increased risk for NHL (hazard ratio=1.7, 95% CI, 1.4-2.2) and Hodgkin lymphoma (HL, hazard ratio=1.4, 95% CI,1.0-2.0). The risk for chronic lymphocytic leukemia (CLL) was increased but was not significant. There was no increased risk of multiple myeloma (MM). Age at diagnosis of case proband did not affect risk in relatives. Relatives of cases with an aggressive subtype of NHL were at further increased risk for developing NHL (hazard ratio=3.6, 95% CI, 1.8-7.0). A manuscript describing the results for MM cases, controls and relatives has been submitted. Relatives of MM cases are at significantly increased risk for developing MM (hazard ratio=1.7, 95% CI, 1.0-2.7) but not for other LP tumors. The association of autoimmune diseases and LP tumors in cases, controls, and relatives has also been examined.

这个遗传流行病学项目中的许多调查来自于对癌症高危家庭的观察或其他病因学研究。来自费城和弗朗西斯科黑色素瘤诊所的718例非西班牙裔白色侵袭性皮肤黑色素瘤患者和来自门诊诊所的945例匹配对照的病例对照研究数据与病例相似的集水区被用于开发风险评估模型，以估计黑色素瘤的绝对风险。以前，在意大利东北部进行的一项对183例黑色素瘤病例和179例对照进行的病例对照研究中，受试者淋巴细胞的DNA修复能力（DRC）显示，总体而言，DRC在病例和对照之间没有差异，但DRC低、晒黑能力差或发育不良痣的受试者患黑色素瘤的风险增加。为了探索这些发现的遗传基础，我们分析了DNA修复基因中的一系列SNPs。在DNA聚合酶或脱碱基修复基因中发现的SNP与黑色素瘤风险降低相关，而NER基因中的SNP与老年受试者的较高风险或DRC降低相关。使用标准化仪器测量受试者的组成性肤色和紫外线敏感度，并分析SNP在潜在色素沉着基因中的作用。结果表明，基于比色计的皮肤亮度和MC 1 R色素沉着基因的变体都与黑色素瘤风险相关，即使在调整传统评估的色素沉着特征后，风险也会随着MC 1 R变体的数量而增加。MC 1 R变异体也与病例中的黑色素瘤厚度相关。作为一项成年脑肿瘤患者的DCEG综合病例对照研究的后续研究，对480例符合条件的胶质瘤病例的父母、兄弟姐妹和成年子女进行了一项以家庭为基础的研究。365例胶质瘤患者的亲属接受了有关个人和家族病史以及其他风险因素的采访，并被要求提供口腔细胞作为DNA来源。正在进行分析，以检查与人口控制相比，一级亲属患癌症的风险。在未来，亲属将被用作关联研究和分析中神经胶质瘤病例的对照，以评估遗传易感性和环境暴露对神经胶质瘤和病因相关肿瘤风险的作用。回顾性审查临床数据，将来自同一机构的33名髓母细胞瘤患者与其46名未受影响的亲属进行比较，发现大多数髓母细胞瘤患者的临床发现很少。结果表明，临床上可识别的综合征是罕见的髓母细胞瘤患者，然而，PTCH 1和SUFU突变是目前在一个低的，但显着frequency.Registry数据从瑞典和丹麦正在继续进行分析。一篇正在出版的论文描述了非霍奇金淋巴瘤（NHL）患者亲属患淋巴增生性（LP）肿瘤的风险。亲属患NHL（风险比=1.7，95%CI，1.4-2.2）和霍奇金淋巴瘤（HL，风险比=1.4，95%CI，1.0 -2.0）的风险显著增加。慢性淋巴细胞白血病（CLL）的风险增加，但不显着。多发性骨髓瘤（MM）的风险没有增加。先证者诊断时的年龄不影响亲属的风险。NHL侵袭性亚型病例的亲属发生NHL的风险进一步增加（风险比=3.6，95%CI，1.8-7.0）。已提交描述MM病例、对照和亲属结果的手稿。MM病例的亲属发生MM的风险显著增加（风险比=1.7，95% CI，1.0-2.7），但其他LP肿瘤的风险不显著增加。自身免疫性疾病和LP肿瘤在病例、对照和亲属中的相关性也进行了研究。