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OmpR and acid regulation of Escherichia coli fim genes

大肠杆菌基因的 OmpR 和酸调节

基本信息

批准号：
7247066
负责人：
WILLIAM R SCHWAN
金额：
$ 18.47万
依托单位：
UNIVERSITY OF WISCONSIN LA CROSSE
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-05-01 至 2011-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7247066
关键词：
Acids Affect Animals Binding Biological Assay Cells Chromosomes Condition Count Cues Deoxyribonuclease I Deoxyribonucleases Down-Regulation Elements Environment Environmental Risk Factor Enzyme Immunoassay Epithelial Cells Escherichia coli Future Gene Expression Gene Proteins Genes Genetic Transcription Grant Growth Human In Vitro Individual Infective cystitis Investigation LacZ Genes Lead Life Measurement Measures Mediating Mus Mutation Numbers OmpR protein Osmolar Concentration Pharmaceutical Preparations Phase Pilum Plasmids Polymerase Chain Reaction Positioning Attribute Prevention strategy Proteins Reading Regulation Reporter Repression Research Reverse Transcriptase Polymerase Chain Reaction Role Site Surface System Testing Therapeutic Intervention Time Transcriptional Regulation United States Urinary tract Urinary tract infection Uropathogenic E. coli Variant Western Blotting Work acid stress in vivo luminescence mutant prevent recombinase

项目摘要

DESCRIPTION (provided by applicant): Uropathogenic Escherichia coli (UPEC) are the leading cause of urinary tract infections in the United States, resulting in millions of cases each year. Attachment to epithelial cells is a vital first step in UPEC induced urinary tract infections. Type 1 pili mediate this binding, and animal studies have shown a critical role for this pilus type in human bladder infections. Expression of type 1 pili is affected by phase variation whereby cells can switch between piliated and nonpiliated states. Nine fim genes are involved in type 1 pilus expression, including the fimA gene that encodes the structural subunits and site-specific recombinases that affect phase variation encoded by fimB and fimE. Several environmental factors influence regulation of these fim genes, but little is known about the environmental cues regulating fim gene expression in UPEC living in the human urinary tract. Because UPEC cause many urinary tract infections each year, this grant proposes to answer how OmpR and acid tolerance gene proteins may be regulating type 1 pilus expression in UPEC by performing DNase footprinting, PCRs, Western blots, and reporter assays to elucidate fimA, fimB, and fimE expression. Moreover, this proposal will examine for the first time the regulation of these three genes in UPEC cells inoculated into murine urinary tracts using a reporter system. How the fim genes are regulated will be uncovered, which could be useful for future prevention strategies that use dietary changes to change the internal environment of the human urinary tract and prevent the UPEC from binding. This research will help us understand the regulation of type 1 pili fim genes in uropathogenic Escherichia coli growing in human urinary tracts. By understanding how the fim genes are regulated, we may be able to treat urinary tract infections in the future through dietary changes or drugs that affect the regulatory cascade that leads to type 1 pilus expression.

描述（由申请人提供）：尿路致病性大肠杆菌（UPEC）是美国尿路感染的主要原因，每年导致数百万例病例。附着于上皮细胞是UPEC诱导尿路感染的重要第一步。1型菌毛介导这种结合，动物研究表明这种菌毛在人类膀胱感染中起关键作用。1型毛的表达受阶段变化的影响，细胞可以在有毛和无毛状态之间切换。9个膜基因参与1型菌毛的表达，包括编码结构亚基的fimA基因和影响由fimB和fme编码的相变异的位点特异性重组酶。多种环境因素影响这些膜基因的调控，但对于人类尿路UPEC中调控膜基因表达的环境因素知之甚少。由于UPEC每年引起许多尿路感染，该资助计划通过dna酶足迹分析、pcr、Western blots和报告基因分析来阐明fimA、fimB和time的表达，来回答OmpR和耐酸基因蛋白如何调节UPEC中1型菌毛的表达。此外，本提案将首次使用报告系统研究这三个基因在UPEC细胞接种到小鼠尿路中的调控。这些膜基因是如何被调控的将被揭示，这可能对未来的预防策略有用，即通过改变饮食来改变人类尿路的内部环境，防止UPEC的结合。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Escherichia coli pyomyositis in an immunocompromised host.

免疫功能低下宿主中的大肠杆菌化脓性肌炎。

DOI：
发表时间：
2011
期刊：
WMJ : official publication of the State Medical Society of Wisconsin
影响因子：
0
作者：
Sharma,Umesh;Schwan,WilliamR;Agger,WilliamA
通讯作者：
Agger,WilliamA

GadE regulates fliC gene transcription and motility in Escherichia coli.

GadE 调节大肠杆菌中的 fliC 基因转录和运动性。

DOI：
10.5495/wjcid.v10.i1.14
发表时间：
2020
期刊：
World journal of clinical infectious diseases
影响因子：
0
作者：
Schwan,WilliamR;Flohr,NicoleL;Multerer,AbigailR;Starkey,JordanC
通讯作者：
Starkey,JordanC

Regulation of Escherichia coli fim gene transcription by GadE and other acid tolerance gene products.

GadE 和其他耐酸基因产物对大肠杆菌 fim 基因转录的调节。

DOI：
10.1099/mic.0.001149
发表时间：
2022
期刊：
Microbiology (Reading, England)
影响因子：
0
作者：
Schwan,WilliamR;Luedtke,Joshua;Engelbrecht,Kathleen;Mollinger,Jeremy;Wheaton,Andrew;Foster,JohnW;Wolchak,Robert
通讯作者：
Wolchak,Robert

Survival of uropathogenic Escherichia coli in the murine urinary tract is dependent on OmpR.