Anti-apoptotic role of raf-1 in beta-cell insulin signaling

raf-1 在 β 细胞胰岛素信号转导中的抗凋亡作用

• 批准号: 7475370
• 负责人: Emilyn Alejandro
• 金额: $ 0.79万
• 依托单位: UNIVERSITY OF BRITISH COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7475370
• 关键词: Adult; American; Americas; Animal Model; Apoptosis; Apoptotic; Autoimmune Process; Beta Cell; Cell Death; Cell Survival; Cell physiology; Cells; Clinical; Condition; Development; Diabetes Mellitus; Diagnosis; Disease; Disruption; Dose; Epidemic; Equilibrium; Event; General Population; Glucose; Goals; Growth Factor; Health; Human; Inherited; Insulin; Insulin Resistance; Insulin-Dependent Diabetes Mellitus; Islets of Langerhans; Islets of Langerhans Transplantation; Knockout Mice; Laboratories; Life; Molecular; Molecular Biology; Mus; Natural regeneration; Nitric Oxide; Non-Insulin-Dependent Diabetes Mellitus; Numbers; Nutrient; Outcome; Oxygen; Pancreas; Pathway interactions; Patients; Phosphotransferases; Physiological; Pima Indian; Population; Prevalence; Proto-Oncogene Proteins c-raf; Protocols documentation; Public Health; Relative (related person); Research; Research Project Grants; Role; Staging; Stimulus; Transplantation; United States; cellular imaging; cytokine; functional loss; improved; insulin secretion; insulin signaling; islet; prevent; protective effect; research study; transcription factor; type I and type II diabetes

Background: Diabetes is a major health problem in United States of America. Despite extensive study, the exact causes of this disease are not known. However, evidence suggests that all forms of diabetes are associated with reduced beta-cell function and survival. Our laboratory has recently shown that insulin itself controls beta-cell survival. Objective of research: The goal of the proposed research is to characterize the mechanisms through which insulin regulates beta-cell survival in human and mouse islets. We hypothesize that insulin may promote beta-cell survival via a kinase called Raf-1. Description of research project: We will utilize molecular biology, live-cell imaging and knockout mice to dissect the role of Raf-1 in anti-apoptotic beta-cell insulin signaling. Relevancy to diabetes: We expect our proposed experiments to yield new mechanistic information regarding insulin as a beta-cell survival factor. Greater understanding of the control of apoptosis in human islets is crucial to prevent beta-cell death in all types of diabetes and to improve clinical islet transplantation.

背景：糖尿病是美国的一个主要健康问题。尽管进行了广泛的研究