Modifying HA biomaterials with collagen-derived peptides

用胶原蛋白衍生肽修饰 HA 生物材料

• 批准号: 7289277
• 负责人: KRISTIN M Walters
• 金额: $ 3万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7289277
• 关键词: Address; Adhesions; Alloys; Binding; Binding Proteins; Binding Sites; Biocompatible Materials; Biomimetics; Blood; Cell Adhesion; Cell Adhesion Molecules; Cell Differentiation process; Cell-Matrix Junction; Cells; Collagen; Data; Dental Implants; Differentiation Antigens; Fibronectins; Germ Cells; Goals; HA coating; Hydroxyapatites; Implant; In Vitro; Integrin Binding; Integrins; Laboratories; Lead; Light; Liquid substance; Mesenchymal Stem Cells; Methods; Molecular Conformation; Morphology; Orthopedics; Osteoblasts; Osteogenesis; Osteotomy; Patients; Peptides; Phase; Play; Polymers; Proteins; RGD (sequence); Rate; Rattus; Research; Serum; Signal Induction; Signal Transduction; Site; Standards of Weights and Measures; Surface; Testing; Thinking; Tissues; Titanium; Vitronectin; base; biomaterial compatibility; bone; bone morphogenetic protein 2; calcium phosphate; cell behavior; in vivo; mimetics; receptor; response; scaffold; surface coating; synthetic peptide

DESCRIPTION (provided by applicant): Synthetic peptides composed of short sequences within adhesion molecules, referred to as "biomimetic peptides", have been used to promote cell adhesion on many different types of implant biomaterials. However, our laboratory has shown that hydroxyapatite (HA) biomaterials have the ability to adsorb cell attachment factors from blood/serum, and this caused us to question whether biomimetic approaches-would be helpful on this biomaterial. We opted to explore the use of mimetic peptides of proteins which were not present in high abundance in the blood, but played a major roll in pre-osteoblast cell differentiation. Collagen is the major component of the organic phase of bone, but is not present in large quantities in the blood. Activation of the integrin receptors which bind collagen I cause downstream signaling effects which lead to an increase in osteoblastic markers. Our goal is to test the efficacy of three collagen I mimetic peptides, to see which, if any, has the ability to increase initial cell adhesion or the rate of differentiation.

描述（由申请人提供）：由粘附分子内的短序列组成的合成肽，称为“仿生肽”，已用于促进细胞粘附在许多不同类型的植入物生物材料上。然而，我们的实验室已经表明，羟基磷灰石（HA）生物材料具有从血液/血清中吸附细胞附着因子的能力，这使我们质疑仿生方法是否对这种生物材料有帮助。我们选择探索使用蛋白质的模拟肽，这些蛋白质在血液中不存在高丰度，但在前成骨细胞分化中发挥了重要作用。胶原蛋白是骨的有机相的主要成分，但在血液中并不大量存在。结合胶原I的整合素受体的活化引起下游信号传导效应，其导致成骨细胞标志物的增加。我们的目标是测试三种胶原蛋白I模拟肽的功效，以观察哪种肽（如果有的话）具有增加初始细胞粘附或分化速率的能力。