Modifying HA biomaterials with collagen-derived peptides

说明书(申请人提供)：由黏附分子内的短序列组成的合成肽，称为仿生肽，已被用于促进细胞在许多不同类型的植入生物材料上的黏附。然而，我们的实验室表明，羟基磷灰石(HA)生物材料具有从血液/血清中吸附细胞附着因子的能力，这引发了我们对仿生方法是否对这种生物材料有帮助的疑问。我们选择探索使用模拟多肽的蛋白质，这些蛋白质在血液中并不存在高丰度，但在成骨细胞前细胞分化中发挥了重要作用。胶原蛋白是骨骼有机相的主要成分，但在血液中并不大量存在。与I型胶原结合的整合素受体的激活引起下游信号转导效应，从而导致成骨细胞标志物的增加。我们的目标是测试三种I型胶原蛋白模拟肽的有效性，看看哪一种具有增加初始细胞黏附或分化速度的能力。

DESCRIPTION (provided by applicant): Synthetic peptides composed of short sequences within adhesion molecules, referred to as "biomimetic peptides", have been used to promote cell adhesion on many different types of implant biomaterials. However, our laboratory has shown that hydroxyapatite (HA) biomaterials have the ability to adsorb cell attachment factors from blood/serum, and this caused us to question whether biomimetic approaches-would be helpful on this biomaterial. We opted to explore the use of mimetic peptides of proteins which were not present in high abundance in the blood, but played a major roll in pre-osteoblast cell differentiation. Collagen is the major component of the organic phase of bone, but is not present in large quantities in the blood. Activation of the integrin receptors which bind collagen I cause downstream signaling effects which lead to an increase in osteoblastic markers. Our goal is to test the efficacy of three collagen I mimetic peptides, to see which, if any, has the ability to increase initial cell adhesion or the rate of differentiation.