Development of Inhibition in the Avian Cochlear Nucleus

描述（由申请人提供）：听觉系统通过计算耳间时间延迟来定位声音的方位。在鸟类听觉脑干中，大细胞核（NM）是这条时间编码通路的第一个中心核。NM包含高度专门化的解剖、突触和内在特性，这些特性在形成抑制反应和维持NM的时间保真度方面发挥关键作用。人们对这些专门化的发展知之甚少。第一个目的是利用体外电生理技术描述鸡纳米抑制发展的时间过程。第二个目的是了解传入兴奋输入在耳囊肿切除后的生理记录抑制发展中的发展重要性。第三个目标是利用RNA干扰技术研究Kv1.1在正常NM表型发展中的作用。编码干扰knal、Kv1.1基因和EGFP报告基因的rna的质粒将在早期胚胎的卵细胞中电穿孔。转染细胞的生理学将通过发育进行检查。假设这些操作可以改变NM的正常发育和对兴奋性和抑制性输入的生理反应。

DESCRIPTION (provided by applicant): The auditory system localizes sound in azimuth by computing interaural time delays. In the avian auditory brainstem, nucleus magnocellularis (NM) is the first central nucleus in this temporal coding pathway. NM contains highly specialized anatomic, synaptic, and intrinsic properties that play key roles in shaping inhibitory responses and maintaining temporal fidelity in NM. The development of these specializations is poorly understood. The first aim is to describe the time-course of the development of inhibition in chick NM using in vitro electrophysiological techniques.. The second aim is to understand the developmental importance of afferent excitatory inputs in the development of inhibition using otocyst removal followed by physiological recordings. The third aim will examine role of Kv1.1 in developing the normal NM phenotype using RNA interference techniques. Plasmids encoding RNAs designed to interfere with the Kcnal, the Kv1.1 gene, and an EGFP reporter gene will be electroporated in ovo in early embryos. The physiology of transfected cells will be examined through development. These manipulations are hypothesized to alter the normal development of NM anatomy and physiological responses to excitatory and inhibitory inputs.