Androgen Regulated Development of the Xenopus Larynx

雄激素调节爪蟾喉的发育

• 批准号: 7186670
• 负责人: Brian Thomas Nasipak
• 金额: $ 4.24万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7186670
• 关键词: Androgen Receptor; Androgens; Animals; Back; Biological Models; Cells; Development; Dominant-Negative Mutation; Fiber; Gene Expression; Gene Targeting; Genes; Genomics; Goals; Green Fluorescent Proteins; Laryngeal muscle structure; Larynx; Mitotic; Modeling; Molecular; Muscle; Muscle Fibers; Myoblasts; Pharmaceutical Preparations; Physiology; Population; Process; Proliferating; Protein Synthesis Inhibitors; Radiation; Role; Sex Characteristics; Slow-Twitch Muscle Fibers; Stanolone; Stem cells; Testing; Transplantation; Xenopus; Xenopus laevis; cell type; irradiation; myogenesis; neoplastic; research study; response

DESCRIPTION (provided by applicant): The laryngeal muscle of the anuran Xenopus laevis undergoes a complete switch from a mix of fast and slow twitch muscle fibers to all fast twitch fibers in response to androgen treatment. The proposed experiments will investigate the role of myogenic stem cells (myoblasts) in this androgen response. Mitotic cells, including myoblasts, are first depleted from larynges by the use of anti-neoplastic drugs and radiation. Myoblasts will then be isolated from larynges of animals expressing green fluorescent protein and transplanted back into irradiated larynges in order to determine their ability to rescue the response to androgen. Further experiments will determine whether myoblasts are the target of androgen by rendering larynges androgen insensitive by means of a dominant negative androgen receptor (dnAR). Myoblasts from wild-type larynges will then be transplanted into those expressing the dnAR in order to determine their ability to rescue the androgen response. The final aim of the application is to determine the androgen responsive target genes responsible for fiber type switching.

描述（由申请人提供）：响应于雄激素治疗，无尾爪蟾的喉部肌肉经历了从快肌纤维和慢肌纤维的混合到所有快肌纤维的完全转变。拟议的实验将研究生肌干细胞（成肌细胞）在这种雄激素反应中的作用。有丝分裂细胞，包括成肌细胞，首先通过使用抗肿瘤药物和辐射从喉部被耗尽。然后将从表达绿色荧光蛋白的动物的喉中分离出成肌细胞，并将其移植回受辐射的喉中，以确定它们挽救雄激素反应的能力。进一步的实验将通过显性失活雄激素受体（dnAR）使喉雄激素不敏感，从而确定成肌细胞是否是雄激素的目标。然后将来自野生型喉的成肌细胞移植到表达 dnAR 的成肌细胞中，以确定它们挽救雄激素反应的能力。该应用的最终目的是确定负责纤维类型转换的雄激素响应靶基因。

项目成果

The genome of the diploid anuran Xenopus tropicalis contains a novel array of sarcoplasmic myosin heavy chain genes expressed in larval muscle and larynx.

二倍体蜉蝣热带爪蟾的基因组包含一系列在幼虫肌肉和喉中表达的新型肌浆肌球蛋白重链基因。

• DOI: 10.1007/s00427-008-0225-0
• 发表时间: 2008
• 期刊: Development genes and evolution
• 影响因子: 2.4
• 作者: Nasipak,BrianT;Kelley,DarcyB
• 通讯作者: Kelley,DarcyB

Developing laryngeal muscle of Xenopus laevis as a model system: androgen-driven myogenesis controls fiber type transformation.

• DOI: 10.1002/dneu.20983
• 发表时间: 2012-04
• 期刊: DEVELOPMENTAL NEUROBIOLOGY
• 影响因子: 3
• 作者: Nasipak, Brian;Kelley, Darcy B.
• 通讯作者: Kelley, Darcy B.