Role of the mPFC-Amygdala Circuit in Fear Extinction

mPFC-杏仁核回路在恐惧消退中的作用

• 批准号: 7127327
• 负责人: EKATERINA LIKHTIK
• 金额: $ 2.07万
• 依托单位: RUTGERS THE STATE UNIV OF NJ NEWARK
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7127327
• 关键词: Accounting; Address; Affect; Agonist; Amygdaloid structure; Animals; Anxiety Disorders; Area; Auditory; Axon; Behavior; Behavioral; Brain Stem; Cell Nucleus; Cells; Chromosome Pairing; Condition; Data; Disruption; Event; Extinction (Psychology); Fright; Goals; Human; Hypothalamic structure; Image; Intercalated Cell; Learning; Lesion; Link; Medial; Mediating; Microinjections; Modeling; Neurons; Output; Play; Post-Traumatic Stress Disorders; Prefrontal Cortex; Process; Public Health; Rattus; Rodent; Role; Sensory; Stimulus; Structure; Synapses; Techniques; Testing; Training; Work; conditioned fear; conditioning; falls; in vivo; mu opioid receptors; neurobiotin; relating to nervous system; research study; response

DESCRIPTION (provided by applicant): The goal of the proposed work is to elucidate the mechanisms underlying extinction of conditioned fear. Since extinction is widely used as a model for the treatment of anxiety disorders such as Post Traumatic Stress Disorder (PTSD), understanding its neural correlates will undoubtedly be beneficial to public health. Cortical influence over the amygdala is known to be inhibitory and [to] suppress conditioned fear, thus serving as the model structure for inhibitory control of amygdala output. The medial prefrontal cortex (mPFC) can inhibit the central nucleus of the amygdala (CEm), which is the main output projecting to downstream targets to produce a fearful response. We will test a model which posits that mPFC-mediated inhibition in the CEm can occur via its projection to small groups of GABAergic cells in the amygdala (the intercalated cell masses (ITCs)), which then project to and inhibit the CEm. ITCs will be lesioned by microinjections of saporin conjugated to an agonist of the mu-opioid receptor (which they abundantly express) and fear conditioning and its extinction will be tested. Electrophysiological recordings will determine whether the lesion has affected the inhibitory drive that the mPFC is known to exert on amygdala output.

描述（由申请人提供）：拟议工作的目标是阐明条件恐惧消退的潜在机制。由于灭绝被广泛用作治疗创伤后应激障碍（PTSD）等焦虑症的模型，因此了解其神经相关性无疑将有利于公共卫生。大脑皮层对杏仁核的影响是抑制性的，可以抑制条件性恐惧，因此可以作为杏仁核输出抑制控制的模型结构。内侧前额叶皮层（mPFC）可以抑制杏仁核中央核（CEm），这是向下游目标投射产生恐惧反应的主要输出。我们将测试一个模型，该模型假定mPFC介导的CEm抑制可以通过其投射到杏仁核中的小组GABA能细胞（嵌入细胞团（ITC）），然后投射到CEm并抑制CEm而发生。将通过显微注射与μ-阿片受体（其大量表达）的激动剂缀合的皂草素来损伤ITC，并测试恐惧条件反射及其消退。电生理记录将确定损伤是否影响了mPFC对杏仁核输出的抑制驱动。