Nanotechnology Strategies for Growth of Bones and Teeth

骨骼和牙齿生长的纳米技术策略

• 批准号: 7448583
• 负责人: SAMUEL I STUPP
• 金额: $ 59.36万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-12 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7448583
• 关键词: Affect; Allografting; Ameloblasts; Autologous; Binding Proteins; Biological; Biological Assay; Biology; Biomedical Research; Biomimetics; Bone Growth; Bone Matrix; Bone Regeneration; Bone Tissue; Cell Adhesion; Cells; Cephalic; Chemistry; Defect; Dental; Dental Enamel; Development; Embryo; Encapsulated; Extracellular Space; Gene Expression; Growth Factor; Habits; Healed; Implant; In Vitro; Integrin Binding; Laboratories; Liquid substance; Modeling; Mus; Nanostructures; Nanotechnology; Natural regeneration; Organ Culture Techniques; Orthopedic Surgery procedures; Osteogenesis; Pattern; Peptides; Performance; Physiological; Production; Proteins; Rattus; Recombinant Proteins; Research; Research Personnel; Signal Transduction; Site; Standards of Weights and Measures; Stem cells; System; Testing; Tissue Transplantation; Tissues; Tooth structure; base; biomineralization; bone; design; healing; in vitro Assay; in vitro Model; in vivo; in vivo regeneration; interest; mineralization; nanofiber; nanolithography; nanoscale; nanoscience; nanostructured; performance tests; programs; repaired; scaffold; self assembly

DESCRIPTION (provided by applicant): This application proposes to develop molecularly designed peptide nanostructures that can trigger differentiation of progenitor cells and mineralization in physiological media with features that mimic enamel or bone matrices. The proposed research is based on the hypothesis that regeneration of enamel and bone can be triggered by templates designed at the nanoscale to present biological signals and induce biomimetic mineralization. Using in vitro assays, we will investigate how these biomimetic mineralization templates affect matrix synthesis by ameloblasts and osteoprogenitor cells, and also test their performance in embryonic tooth culture and in vivo bone repair models. This research will guide the development of synthetic, versatile, nanostructured templates that can be used for hard tissue (enamel or bone) regeneration and repair without the need for autologous or autogenic tissue transplantation. The proposed program's specific aims include: development of peptide amphiphiles that will self-assemble in physiological fluids and trigger biomimetic mineralization of enamel- or bone-like matrices, testing of these nanofiber systems in vitro with osteoprogenitor and ameloblast cells using mineralization, protein and gene expression assays, and using patterned substrates or scaffolds of peptide amphiphile nanofibers for in vitro and in vivo regeneration of dental and bone tissues in murine models. The proposed research program is a collaborative effort of four investigators specializing in self-assembly/materials chemistry, dental tissue biology and orthopedic surgery, whose interests intersect on the focused application of nanoscience and nanotechnology to biomedical research.

描述（由申请人提供）：本申请提出开发分子设计的肽纳米结构，其可以在具有模拟釉质或骨基质的特征的生理介质中触发祖细胞的分化和矿化。这项研究基于这样的假设，即牙釉质和骨的再生可以通过纳米级设计的模板来触发，以呈现生物信号并诱导仿生矿化。使用体外试验，我们将研究这些仿生矿化模板如何影响成釉细胞和骨祖细胞的基质合成，并测试它们在胚胎牙齿培养和体内骨修复模型中的性能。这项研究将指导合成，通用，纳米结构模板的开发，可用于硬组织（牙釉质或骨）再生和修复，而无需自体或自体组织移植。拟议方案的具体目标包括：开发肽两亲物，其将在生理流体中自组装并触发釉质或骨样基质的仿生矿化，使用矿化、蛋白质和基因表达测定在体外用骨祖细胞和成釉细胞测试这些生物活性系统，以及使用肽两亲物纳米纤维的图案化基底或支架在鼠模型中进行牙和骨组织的体外和体内再生。拟议的研究计划是四名专门从事自组装/材料化学，牙科组织生物学和整形外科的研究人员的合作努力，他们的兴趣集中在纳米科学和纳米技术在生物医学研究中的应用。