Nanotechnology Strategies for Growth of Bones and Teeth

骨骼和牙齿生长的纳米技术策略

• 批准号: 8260509
• 负责人: SAMUEL I STUPP
• 金额: $ 52.88万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-12 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8260509
• 关键词: Adult; Architecture; Binding; Biological; Biological Models; Bioreactors; Bone Growth; Bone Regeneration; Cells; Characteristics; Clinical; Clinical Trials; Complex; Defect; Dental; Dental Enamel; Deposition; Development; Developmental Biology; Disease; Enamel Formation; Engineering; Environment; Epitopes; Event; Extracellular Matrix; Filament; Funding; Future; Gene Expression; Genes; Genetic; Goals; Grant; Growth Factor; Healthcare; Human; Human body; Hybrids; Hydroxyapatites; Implant; In Situ; In Vitro; Incisor; Individual; Injury; Island; Kidney; Lead; Length; Libraries; Life Expectancy; Life Style; Maintenance; Measures; Mediating; Methodology; Methods; Minerals; Modeling; Molecular; Molecular Profiling; Mus; Nanostructures; Nanotechnology; Natural regeneration; Osteogenesis; Pathway interactions; Peptide Signal Sequences; Peptides; Production; Productivity; Property; Proteins; Rattus; Regenerative Medicine; Relative (related person); Signal Transduction; Signaling Pathway Gene; Small Interfering RNA; Structure; Surface; Synthesis Chemistry; System; Technology; Testing; Therapeutic; Tissues; Tooth structure; Translations; Trauma; Weight-Bearing state; age related; amelogenin; base; bone; bone morphogenetic protein 2; capsule; clinical application; design; extracellular; improved; in vivo; interdisciplinary approach; leucine-rich amelogenin peptide; millimeter; mimetics; mineralization; nanofiber; nanoscale; nanoscience; novel; osteogenic; performance tests; protein aminoacid sequence; public health relevance; regenerative; repaired; research study; response; scaffold; skeletal disorder; tissue regeneration

DESCRIPTION (provided by applicant): Hard tissues in the human body, such as bone and tooth enamel, are architecturally highly complex tissues with superior strength modulus and rigidity compared to other tissues. Their formation involves specific and tightly regulated molecular events between cells and their surrounding extracellular environments. Following injury or disease, the adult human body cannot initiate molecular mechanisms for repair similar to those that occur during initial hard tissue development. The emerging field of regenerative medicine aims at the successful structural and functional replacement of tissues lost to trauma or disease. With life expectancy increasing worldwide, age related tissue degradation, injury, or disease of skeletal and dental tissues pose a significant expense to healthcare, individual productivity, and the maintenance of an active lifestyle. In response to this pressing need, breakthroughs are needed to transform the strategies used for hard tissue regeneration. Our collaborative team seeks to uncover principles governing this regenerative response in hard tissue using three-dimensional self-assembling bioactive scaffolds as a model therapeutic material. Using a multidisciplinary approach spanning the fields of nanoscience, synthetic chemistry, genetics, and developmental biology, we propose the development of highly bioactive materials containing bottom up designed nanostructures with potential to effectively regenerate bone and tooth enamel. Our team aims to accomplish three main goals: 1) use rational molecular design to optimize new materials that can trigger the regeneration of bone and enamel, including the development of artificial substitutes that emulate the architecture of hard tissue matrices; 2) improve our understanding of the cellular and molecular mechanisms operating during hard tissue development and regeneration in order to optimize clinical regenerative strategies; and 3) assess the scalability of our technology toward future clinical trials. PUBLIC HEALTH RELEVANCE: Following injury or disease in hard tissues such as bone and tooth enamel, the adult human body cannot initiate repair mechanisms similar to those that occur during development. Our collaborative team uses nanoscience, synthetic chemistry, genetics, and developmental biology to engineer biologically instructive scaffolds for cells targeting bone and enamel formation. We pursue three main goals: 1) use rational design to optimize materials for use in methods to regenerate bone and enamel; 2) identify and employ cellular and molecular mechanisms operating during hard tissue regeneration so as to optimize clinical regenerative strategies; and 3) assess the scalability of our technology toward future clinical trials.

描述（由申请人提供）：人体中的硬组织，如骨和牙釉质，是结构上高度复杂的组织，与其他组织相比具有上级强度模量和刚度。它们的形成涉及细胞及其周围细胞外环境之间的特异性和严格调节的分子事件。在受伤或疾病之后，成年人体不能启动类似于在最初的硬组织发育期间发生的分子修复机制。再生医学的新兴领域旨在成功地替代因创伤或疾病而失去的组织的结构和功能。随着世界范围内预期寿命的增加，与年龄相关的组织退化、骨骼和牙齿组织的损伤或疾病对医疗保健、个人生产力和维持积极的生活方式造成了巨大的费用。为了满足这一迫切需求，需要突破性地改变用于硬组织再生的策略。我们的合作团队试图揭示使用三维自组装生物活性支架作为模型治疗材料在硬组织中控制这种再生反应的原理。使用跨纳米科学，合成化学，遗传学和发育生物学领域的多学科方法，我们提出了开发含有自下而上设计的纳米结构的高生物活性材料，具有有效再生骨骼和牙釉质的潜力。我们的团队旨在实现三个主要目标：1）使用合理的分子设计来优化可以引发骨和釉质再生的新材料，包括开发模拟硬组织基质结构的人工替代品; 2）提高我们对硬组织发育和再生过程中细胞和分子机制的理解，以优化临床再生策略;以及3）评估我们的技术对未来临床试验的可扩展性。 公共卫生关系：在硬组织（如骨骼和牙釉质）受伤或患病后，成年人体无法启动类似于发育期间发生的修复机制。我们的合作团队使用纳米科学，合成化学，遗传学和发育生物学，为靶向骨和釉质形成的细胞设计生物学指导性支架。我们追求三个主要目标：1）使用合理的设计来优化用于再生骨和牙釉质的方法的材料; 2）识别和采用在硬组织再生过程中操作的细胞和分子机制，以优化临床再生策略; 3）评估我们的技术对未来临床试验的可扩展性。