MOLECULAR PACKING AND ORIENTATION OF SELF-ASSEMBLED PEPTIDE AMPHIPHILE SYSTEM

自组装肽两亲体系的分子堆积和取向

• 批准号: 8363691
• 负责人: SAMUEL I STUPP
• 金额: $ 1.22万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363691
• 关键词: Biocompatible Materials; Biological; Biology; Biomedical Engineering; Biomimetic Materials; Cells; Collection; Crystallization; Dental Enamel; Deposition; Development; Drug vehicle; Enamel Formation; Exhibits; Funding; Goals; Grant; Growth; Individual; Injury; Laboratories; Length; Mechanics; Minerals; Molecular; National Center for Research Resources; Peptides; Principal Investigator; Process; Property; Proteins; Research; Research Infrastructure; Resources; Scaffolding Protein; Source; Structure; Structure-Activity Relationship; System; Tissues; Tooth structure; United States National Institutes of Health; X ray diffraction analysis; X-Ray Diffraction; biomineralization; bone; carboapatite; cell motility; cost; mineralization; nanoscale; novel; research study; response; scaffold; stem cell differentiation; structural biology; tissue regeneration

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Biological minerals such as vertebrate bone and tooth exhibit remarkable levels of hierarchy that are controlled over multiple length scales to produce superior mechanical properties compared to their individual building blocks. The process of mineralization involves the deposition of a protein scaffold upon which controlled crystallization of carbonated apatite occurs. In an effort to create biomimetic materials for use with mineralized tissues our lab is exploring the use of peptide amphiphiles as a synthetic scaffold. These molecules have been utilized by the Stupp laboratory to achieve a collection of bioengineering goals: as cell and tissue artificial scaffolds; as biomaterials that direct stem cell differentiation cell migration cellular response and tissue regeneration after injury; as vehicles for drug cell peptide and protein delivery [8-12]; and as materials that induce biomineralization for bone and tooth enamel formation [13-15]. The proposed experiments focus on understanding the supramolecular structures that these PA molecules create. Additionally we plan to utilize PA scaffolds as a structural matrix for supporting nucleation and growth of biologically relevant mineral and characterize the inorganic-organic relationships at the nanoscale using X-ray diffraction. Understanding the assembly of peptide amphiphiles and interactions occurring at the organic-inorganic interface in biomineralized structures can elucidate structure-function relationships achieved by biology and enable the development of novel intelligent materials.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 脊椎动物骨和牙齿等生物矿物质表现出显着水平的层次结构，这些层次结构在多个长度尺度上受到控制，与单独的构件相比，它们在多个长度尺度上产生了出色的机械性能。矿化过程涉及蛋白支架的沉积，在该蛋白质支架上发生碳酸磷灰石的结晶。为了创建与矿化组织一起使用的仿生材料，我们的实验室正在探索将肽两亲物用作合成支架的使用。这些分子已被Stupp实验室利用来实现生物工程目标的集合：作为细胞和组织人造支架；作为直接干细胞分化细胞迁移的生物材料，损伤后细胞迁移反应和组织再生；作为药物细胞肽和蛋白质递送的车辆[8-12]；作为诱导骨骼和牙齿搪瓷形成生物矿化的材料[13-15]。提出的实验着重于理解这些PA分子产生的超分子结构。此外，我们计划利用PA支架作为结构基质，以支持生物学相关矿物质的成核和生长，并使用X射线衍射在纳米级处表征无机有机关系。了解生物矿化结构中有机无机界面上发生的肽两亲物的组装以及相互作用可以阐明生物学实现的结构 - 功能关系，并促进新型智能材料的发展。