MOLECULAR PACKING AND ORIENTATION OF SELF-ASSEMBLED PEPTIDE AMPHIPHILE SYSTEM

自组装肽两亲体系的分子堆积和取向

• 批准号: 8363691
• 负责人: SAMUEL I STUPP
• 金额: $ 1.22万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363691
• 关键词: Biocompatible Materials; Biological; Biology; Biomedical Engineering; Biomimetic Materials; Cells; Collection; Crystallization; Dental Enamel; Deposition; Development; Drug vehicle; Enamel Formation; Exhibits; Funding; Goals; Grant; Growth; Individual; Injury; Laboratories; Length; Mechanics; Minerals; Molecular; National Center for Research Resources; Peptides; Principal Investigator; Process; Property; Proteins; Research; Research Infrastructure; Resources; Scaffolding Protein; Source; Structure; Structure-Activity Relationship; System; Tissues; Tooth structure; United States National Institutes of Health; X ray diffraction analysis; X-Ray Diffraction; biomineralization; bone; carboapatite; cell motility; cost; mineralization; nanoscale; novel; research study; response; scaffold; stem cell differentiation; structural biology; tissue regeneration

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Biological minerals such as vertebrate bone and tooth exhibit remarkable levels of hierarchy that are controlled over multiple length scales to produce superior mechanical properties compared to their individual building blocks. The process of mineralization involves the deposition of a protein scaffold upon which controlled crystallization of carbonated apatite occurs. In an effort to create biomimetic materials for use with mineralized tissues our lab is exploring the use of peptide amphiphiles as a synthetic scaffold. These molecules have been utilized by the Stupp laboratory to achieve a collection of bioengineering goals: as cell and tissue artificial scaffolds; as biomaterials that direct stem cell differentiation cell migration cellular response and tissue regeneration after injury; as vehicles for drug cell peptide and protein delivery [8-12]; and as materials that induce biomineralization for bone and tooth enamel formation [13-15]. The proposed experiments focus on understanding the supramolecular structures that these PA molecules create. Additionally we plan to utilize PA scaffolds as a structural matrix for supporting nucleation and growth of biologically relevant mineral and characterize the inorganic-organic relationships at the nanoscale using X-ray diffraction. Understanding the assembly of peptide amphiphiles and interactions occurring at the organic-inorganic interface in biomineralized structures can elucidate structure-function relationships achieved by biology and enable the development of novel intelligent materials.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 生物矿物质如脊椎动物的骨骼和牙齿表现出显著的层次结构，这些层次结构在多个长度尺度上受到控制，以产生与其单个构建块相比上级的机械性能。矿化过程涉及蛋白质支架的沉积，在蛋白质支架上发生碳酸磷灰石的受控结晶。为了创造用于矿化组织的仿生材料，我们实验室正在探索使用肽两亲物作为合成支架。这些分子已被Stupp实验室用于实现一系列生物工程目标：作为细胞和组织人工支架;作为指导干细胞分化、细胞迁移、细胞反应和损伤后组织再生的生物材料;作为药物细胞肽和蛋白质递送的载体[8-12];以及作为诱导骨和牙釉质形成的生物矿化的材料[13-15]。拟议的实验重点是了解这些PA分子创建的超分子结构。此外，我们计划利用PA支架作为结构基质，用于支持生物相关矿物的成核和生长，并使用X射线衍射在纳米级上表征无机-有机关系。了解肽两亲物的组装和生物矿化结构中有机-无机界面处发生的相互作用可以阐明生物学实现的结构-功能关系，并能够开发新型智能材料。