MOLECULAR PACKING AND ORIENTATION OF SELF-ASSEMBLED PEPTIDE AMPHIPHILE SYSTEM

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 脊椎动物骨骼和牙齿等生物矿物表现出惊人的等级水平，这些等级在多个长度尺度上受到控制，与它们各自的构建块相比，可以产生更好的机械性能。矿化过程涉及蛋白质支架的沉积，碳化磷灰石在其上发生受控结晶。为了创造用于矿化组织的仿生材料，我们的实验室正在探索将多肽两亲分子用作合成支架。Stupp实验室利用这些分子实现了一系列生物工程目标：作为细胞和组织人工支架；作为引导干细胞分化、细胞迁移、细胞反应和损伤后组织再生的生物材料；作为药物细胞肽和蛋白质输送的载体[8-12]；以及作为诱导生物矿化形成骨和牙釉质的材料[13-15]。拟议的实验重点是了解这些PA分子创造的超分子结构。此外，我们计划利用PA支架作为结构基质来支持生物相关矿物的成核和生长，并使用X射线衍射在纳米尺度上表征无机-有机关系。了解生物矿化结构中两亲肽的组装和在有机-无机界面上发生的相互作用，可以阐明生物实现的结构-功能关系，并使新型智能材料的开发成为可能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Biological minerals such as vertebrate bone and tooth exhibit remarkable levels of hierarchy that are controlled over multiple length scales to produce superior mechanical properties compared to their individual building blocks. The process of mineralization involves the deposition of a protein scaffold upon which controlled crystallization of carbonated apatite occurs. In an effort to create biomimetic materials for use with mineralized tissues our lab is exploring the use of peptide amphiphiles as a synthetic scaffold. These molecules have been utilized by the Stupp laboratory to achieve a collection of bioengineering goals: as cell and tissue artificial scaffolds; as biomaterials that direct stem cell differentiation cell migration cellular response and tissue regeneration after injury; as vehicles for drug cell peptide and protein delivery [8-12]; and as materials that induce biomineralization for bone and tooth enamel formation [13-15]. The proposed experiments focus on understanding the supramolecular structures that these PA molecules create. Additionally we plan to utilize PA scaffolds as a structural matrix for supporting nucleation and growth of biologically relevant mineral and characterize the inorganic-organic relationships at the nanoscale using X-ray diffraction. Understanding the assembly of peptide amphiphiles and interactions occurring at the organic-inorganic interface in biomineralized structures can elucidate structure-function relationships achieved by biology and enable the development of novel intelligent materials.