Rapid Detection and Monitoring of Biomarkers for Traumatic Brain Injury

快速检测和监测创伤性脑损伤的生物标志物

• 批准号: 7324679
• 负责人: William Lawrence
• 金额: $ 9.17万
• 依托单位: RADIATION MONITORING DEVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-25 至 2008-09-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7324679
• 关键词: Antibodies; Apoptosis; Biological Assay; Biological Markers; Blood specimen; Boston; Bovine Serum Albumin; Brain Injuries; Cephalic; Cerebrospinal Fluid; Cerebrum; Clinical; Collaborations; Collection; Complex; Condition; Count; Craniocerebral Trauma; Data Analyses; Detection; Development; Devices; Diagnosis; Diagnostic; Evaluation; Evaluation Research; Event; Government; Hemorrhage; Hospitalization; Hospitals; Immobilization; Inflammation; Ion Transport; Label; Laboratories; Light; Magnetic Resonance Imaging; Measurement; Measures; Microfluidic Microchips; Microfluidics; Modeling; Molecular; Monitor; Monoclonal Antibodies; Necrosis; Nervous System Trauma; Optics; Palm-Top Computers; Patients; Performance; Personal Digital Assistant; Persons; Phase; Photons; Placement; Process; Proteins; Proteomics; Purpose; Radiation Monitoring; Range; Reading; Rehabilitation therapy; Running; Sampling; Semiconductors; Serum; Signal Transduction; Silicon; Source; Specific qualifier value; Specificity; Speed; Swelling; System; Techniques; Technology; Testing; Time; Transport Process; Traumatic Brain Injury; Woman; Work; base; clinically significant; computer data analysis; cost; design; detector; injured; instrumentation; interest; lens; metal oxide; microchip; monitoring device; neuroimaging; neurosurgery; novel; pressure; rapid detection; relating to nervous system; response; sensor; size; software development

DESCRIPTION (provided by applicant): Over 370,000 people in the US are hospitalized for traumatic brain injury (TBI) each year. In the US, the cost associated with the treatment, hospitalization and rehabilitation of TBI are estimated to be $48.3 billion annually. A fast, easy, accurate and effective test for brain injury is required to identify head trauma and monitor the progression of the condition. Once the primary impact associated with TBI occurs, rapid response is critical to limit the impact of the secondary damage due to increased cranial pressure and abnormal changes in ion transport processes. Neuroproteomic changes in injured patients underlie the complex molecular events that are indicative of TBI. The high- speed and high-immediacy assessment of these proteomic changes should permit the specific and sensitive diagnosis of TBI and continued evaluation of the condition. Radiation Monitoring Devices proposes to develop a compact, disposable microfluidic sensor that will rapidly detect a panel of multiple TBI biomarkers from a blood sample. To accomplish this, we will integrate silicon-based CMOS (complementary metal oxide semiconductor) photon counting optical (GPD) detectors directly into a microfluidic chip which will detect target protein components with very high sensitivity using fluorescently label monoclonal antibodies. This high sensitivity is achieved by the placement of the photon detectors very close to the source of the fluorescent signal within the microfluidic channel. In Phase I, we will modify an existing microfluidic sensor to develop a test platform to demonstrate the integration of the photon-counting detector, and measure clinically significant concentrations of TBI biomarkers in model samples. In Phase II, we will optimize the design of the microfluidic system, develop software to run on a "palmtop" computer for data analysis and user interface, and measure TBI biomarkers in cerebral spinal fluid. The resulting device will be a general technology platform for the detection of multiple proteins in a single unit. RMD proposes to develop a compact disposable sensor that will rapidly detect biomarkers for traumatic brain injury. The sensor will simultaneously detect a panel of ten to twenty protein biomarkers that are used to diagnose and monitor brain injury. The sensor is based RMDs proprietary photon counting detector array technology.

描述（由申请人提供）：美国每年有超过37万人因创伤性脑损伤（TBI）住院。在美国，与TBI的治疗、住院和康复相关的费用估计为每年483亿美元。需要一种快速，简单，准确和有效的脑损伤测试来识别头部创伤并监测病情的进展。一旦发生与TBI相关的主要影响，快速反应对于限制由于颅压增加和离子传输过程异常变化引起的二次损伤的影响至关重要。损伤患者的神经蛋白质组学变化是指示TBI的复杂分子事件的基础。这些蛋白质组变化的高速和高即时性评估应允许特异性和灵敏性诊断TBI和持续评估病情。Radiation Monitoring Devices提出开发一种紧凑的一次性微流体传感器，该传感器将从血液样本中快速检测一组多个TBI生物标志物。为了实现这一目标，我们将基于硅的CMOS（互补金属氧化物半导体）光子计数光学（GPD）检测器直接集成到微流控芯片中，该芯片将使用荧光标记的单克隆抗体以非常高的灵敏度检测目标蛋白质组分。这种高灵敏度是通过将光子检测器放置在非常靠近微流体通道内的荧光信号源来实现的。在第一阶段，我们将修改现有的微流体传感器，以开发一个测试平台来展示光子计数检测器的集成，并测量模型样本中TBI生物标志物的临床显著浓度。在第二阶段，我们将优化微流控系统的设计，开发在“掌上电脑”上运行的软件，用于数据分析和用户界面，并测量脑脊液中的TBI生物标志物。由此产生的设备将是一个通用的技术平台，用于在单个单元中检测多种蛋白质。 RMD提出开发一种紧凑的一次性传感器，可以快速检测创伤性脑损伤的生物标志物。该传感器将同时检测一组10到20种蛋白质生物标志物，用于诊断和监测脑损伤。该传感器基于RMD专有的光子计数探测器阵列技术。