High Sensitivity Multi-Well Plate Reader Based on an Array of Photon Counting Det

说明书(申请人提供)：标题：高通量和高灵敏度多孔板阅读器项目摘要/摘要：多孔板或微孔板在研究和临床实验室中已变得普遍，用于高速和重复性地处理多个样本。这些板具有标准尺寸(86 X 128 Mm)，有24、96、384或1536孔配置可供选择。在每口井中进行单一的实验或化验，通常使用光学检测技术来评估化验的结果。典型的光学检测方法包括吸光度、荧光强度和偏振、化学发光、荧光共振能量转移(FRET)和时间分辨荧光。目前用于这些微孔板的光学检测系统是基于在速度和灵敏度之间折衷的检测器。成像CCD阵列可以同时对平板上的所有井进行成像，但灵敏度低，时间分辨率有限。基于光电倍增管的探测器系统具有高灵敏度和快速的时间响应，但一次只能探测一口井。辐射监测设备公司计划开发一种用于高灵敏度多孔板读取器的探测器系统，该系统基于我们的CMOS型光子计数盖革模式雪崩光电二极管阵列。建议的探测器将结合电荷耦合器件的高通量和光电倍增管的高灵敏度。此外，探测器阵列可以测量2纳秒的荧光寿命，这是CCD阵列无法做到的，并且具有比光电倍增管更好的近红外灵敏度。第一阶段SBIR的目标是开发一个使用RMD盖革模式雪崩光电二极管阵列探测器的24孔板读取器原型。第一阶段计划的关键挑战将是建立原型探测器和集成数字测量功能。所提出的平板阅读器将使用24个单元的雪崩光电二极管探测器同时测量24孔板中所有样品的荧光强度或荧光寿命信号。第二阶段计划的目标将是建立一种完全集成的平板读取器解决方案，可以读取24、96或384孔板，并同时测量每个孔处的荧光强度、偏振转移和荧光寿命。在第二阶段计划中，我们计划增加阵列中的像素数量，增加每个像素的功能，并减小每个像素的大小。标题：高通量和高灵敏度多孔平板阅读器项目简介：多孔或微孔平板已经在研究和临床实验室中变得普遍，以高速和重复性处理多个样本。辐射监测设备公司计划开发一种用于高灵敏度多孔板读取器的探测器系统，该系统基于我们的CMOS型光子计数盖革模式雪崩光电二极管阵列。拟议的探测器将具有比现有探测器更好的功能，并将具有更低的成本。

DESCRIPTION (provided by applicant): Title: High throughput and high sensitivity multi-well plate reader Project Summary/Abstract: The multi-well or microwell plate has become ubiquitous in research and clinical laboratories for processing multiple samples with high speed and reproducibility. These plates have a standard size (86 x 128 mm) and are available in 24, 96, 384, or 1536 well configurations. A single experiment or assay is carried out in each well and the results of the assay are most often evaluated using an optical detection technique. Typical optical detection methods include absorbance, fluorescence intensity and polarization, chemiluminescence, fluorescence resonance energy transfer (FRET) and time resolved fluorescence. Current optical detection systems for these microwell plates are based on detectors that are a compromise between speed and sensitivity. Imaging CCD arrays can evaluate all of the wells in a plate simultaneously, but have low sensitivity and limited temporal resolution. PMT based detector systems have high sensitivity and fast temporal response, but can only interrogate one well at a time. Radiation Monitoring Devices proposes to develop a detector system for a high sensitivity multi-well plate reader based on an array of our CMOS photon counting Geiger mode avalanche photodiodes. The proposed detector would incorporate the high throughput of the CCD detector with the high sensitivity of the PMT. In addition, the detector array can measure fluorescent lifetimes of 2 nanoseconds, which cannot be done with CCD arrays and has better near infrared sensitivity than the PMT. The goal of the Phase I SBIR is to develop a prototype 24 well plate reader using the RMD Geiger mode Avalanche Photodiode array detector. The key challenges in the Phase I program will be to build the prototype detector and integrate the digital measurement functions. The proposed plate reader would simultaneously measure fluorescence intensity or fluorescence lifetime signals from all of the samples in the 24 well plate using a 24 element CMOS APD detector. The goal of the Phase II program will be to build a fully integrated plate reader solution which can read 24, 96, or 384 well plates and simultaneously measure fluorescence intensity, polarization transfer, and fluorescence lifetime at each well. In the Phase II program, we plan to increase the number of pixels in the array, increase the functionality of each pixel, and reduce the size of each pixel. Title: High throughput and high sensitivity multi-well plate reader Project Narrative: The multi-well or microwell plate has become ubiquitous in research and clinical laboratories for processing multiple samples with high speed and reproducibility. Radiation Monitoring devices proposes to develop a detector system for a high sensitivity multi-well plate reader based on an array of our CMOS photon counting Geiger mode avalanche photodiodes. The proposed detector will have improved functionality over existing detectors and will have lower cost.