High Sensitivity Multi-Well Plate Reader Based on an Array of Photon Counting Det

基于光子计数探测器阵列的高灵敏度多孔板读数器

• 批准号: 7325587
• 负责人: William Lawrence
• 金额: $ 13.5万
• 依托单位: RADIATION MONITORING DEVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-10 至 2008-09-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7325587
• 关键词: Biological Assay; Biological Models; Clinical; Count; Data; Detection; Development; Electronics; Elements; Evaluation; Fluorescence; Fluorescence Resonance Energy Transfer; Goals; Government; Image; Laboratories; Measurement; Measures; Methods; Numbers; Optics; Performance; Persons; Phase; Photons; Process; Purpose; Radiation Monitoring; Range; Reader; Reading; Reproducibility; Research; Resolution; Sampling; Semiconductors; Signal Transduction; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Solutions; Speed; Standards of Weights and Measures; System; Techniques; Technology; Testing; Time; Title; abstracting; base; cost; design; detector; digital; improved; metal oxide; monitoring device; nanosecond; programs; prototype; research study; response; size

DESCRIPTION (provided by applicant): Title: High throughput and high sensitivity multi-well plate reader Project Summary/Abstract: The multi-well or microwell plate has become ubiquitous in research and clinical laboratories for processing multiple samples with high speed and reproducibility. These plates have a standard size (86 x 128 mm) and are available in 24, 96, 384, or 1536 well configurations. A single experiment or assay is carried out in each well and the results of the assay are most often evaluated using an optical detection technique. Typical optical detection methods include absorbance, fluorescence intensity and polarization, chemiluminescence, fluorescence resonance energy transfer (FRET) and time resolved fluorescence. Current optical detection systems for these microwell plates are based on detectors that are a compromise between speed and sensitivity. Imaging CCD arrays can evaluate all of the wells in a plate simultaneously, but have low sensitivity and limited temporal resolution. PMT based detector systems have high sensitivity and fast temporal response, but can only interrogate one well at a time. Radiation Monitoring Devices proposes to develop a detector system for a high sensitivity multi-well plate reader based on an array of our CMOS photon counting Geiger mode avalanche photodiodes. The proposed detector would incorporate the high throughput of the CCD detector with the high sensitivity of the PMT. In addition, the detector array can measure fluorescent lifetimes of 2 nanoseconds, which cannot be done with CCD arrays and has better near infrared sensitivity than the PMT. The goal of the Phase I SBIR is to develop a prototype 24 well plate reader using the RMD Geiger mode Avalanche Photodiode array detector. The key challenges in the Phase I program will be to build the prototype detector and integrate the digital measurement functions. The proposed plate reader would simultaneously measure fluorescence intensity or fluorescence lifetime signals from all of the samples in the 24 well plate using a 24 element CMOS APD detector. The goal of the Phase II program will be to build a fully integrated plate reader solution which can read 24, 96, or 384 well plates and simultaneously measure fluorescence intensity, polarization transfer, and fluorescence lifetime at each well. In the Phase II program, we plan to increase the number of pixels in the array, increase the functionality of each pixel, and reduce the size of each pixel. Title: High throughput and high sensitivity multi-well plate reader Project Narrative: The multi-well or microwell plate has become ubiquitous in research and clinical laboratories for processing multiple samples with high speed and reproducibility. Radiation Monitoring devices proposes to develop a detector system for a high sensitivity multi-well plate reader based on an array of our CMOS photon counting Geiger mode avalanche photodiodes. The proposed detector will have improved functionality over existing detectors and will have lower cost.

描述（由申请人提供）：标题：高通量和高灵敏度多孔板读数器项目总结/摘要：多孔或微孔板在研究和临床实验室中已变得无处不在，用于以高速和再现性处理多个样品。这些微孔板具有标准尺寸（86 x 128 mm），并提供24、96、384或1536孔配置。在每个孔中进行单个实验或测定，并且最常使用光学检测技术评价测定的结果。典型的光学检测方法包括吸光度、荧光强度和偏振、化学发光、荧光共振能量转移（FRET）和时间分辨荧光。用于这些微孔板的当前光学检测系统基于在速度和灵敏度之间折衷的检测器。成像CCD阵列可以同时评估板中的所有威尔斯孔，但是具有低灵敏度和有限的时间分辨率。基于PMT的检测器系统具有高灵敏度和快速的时间响应，但一次只能询问一个井。Radiation Monitoring Devices提出开发一种用于高灵敏度多孔板读取器的检测器系统，该系统基于我们的CMOS光子计数盖革模式雪崩光电二极管阵列。所提出的检测器将结合CCD检测器的高通量与PMT的高灵敏度。此外，探测器阵列可以测量2纳秒的荧光寿命，这是CCD阵列无法做到的，并且具有比PMT更好的近红外灵敏度。第I阶段SBIR的目标是使用RMD盖革模式雪崩光电二极管阵列检测器开发原型24孔酶标仪。第一阶段计划的主要挑战将是建立原型探测器并集成数字测量功能。所提出的读板器将使用24元件CMOS APD检测器同时测量来自24孔板中的所有样品的荧光强度或荧光寿命信号。II期项目的目标是构建一个完全集成的酶标仪解决方案，该解决方案可以读取24、96或384孔板，并同时测量每个孔的荧光强度、偏振转移和荧光寿命。在第二阶段计划中，我们计划增加阵列中的像素数量，增加每个像素的功能，并减少每个像素的大小。职务名称：高通量和高灵敏度多孔板读取器项目叙述：多孔板或微孔板在研究和临床实验室中已经变得无处不在，用于以高速和再现性处理多个样品。Radiation Monitoring devices提出开发一种用于高灵敏度多孔板读取器的检测器系统，该系统基于我们的CMOS光子计数盖革模式雪崩光电二极管阵列。所提出的探测器将具有比现有探测器更好的功能，并且将具有更低的成本。