QTOF MASS SPECTROMETER: CELL MOLECULAR BIOLOGY, PROTEIN STRUCTURE

QTOF 质谱仪：细胞分子生物学、蛋白质结构

• 批准号: 7335136
• 负责人: Evan R Williams
• 金额: $ 49.92万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2007-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335136
• 关键词: carbohydrates; gait; housing; ionization; mass spectrometry; molecular biology; oligonucleotides; protein structure; proteins; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The University of California, Berkeley, College of Chemistry and the California Institute for Quantitative Biomedical Research (QB3) are seeking funds to purchase a Q-TOF mass spectrometer equipped with an electrospray ionization source. This instrument will be housed in the Mass Spectrometry Center of Excellence and will replace a 14-year-old instrument that lacks the capabilities necessary to serve researchers' needs. The new Mass Spectrometry Center of Excellence will replace the current College of Chemistry Mass Spectrometry Facility, where most of the staff-run mass spectrometers fail to meet the needs of modern biochemical and biological research. The new Center will address a critical need for state-of-the-art instrumentation to meet the growing demand for analysis of noncovalent complexes and structural characterization of limited amounts of chemical and biochemical compounds from its NIH-funded researchers. The proposed instrument will dramatically improve the speed and accuracy with which mass analysis can be done, and will make possible accurate mass analysis of small amounts of oligonucleotides, proteins, carbohydrates, and bio-inorganic complexes. The research of six faculty members, each of whom would benefit greatly from an in-house, state-of-the- art electrospray mass spectrometer, is described. Five of these researchers have established NIH funding, while one has successfully secured NIH funding in the past. These six groups include a total of 130 postdoctoral researchers, graduate students, and undergraduate students who will benefit directly from this equipment.

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：加州大学伯克利分校化学学院和加州定量生物医学研究所（QB 3）正在寻求资金购买配备电喷雾电离源的Q-TOF质谱仪。该仪器将被安置在质谱卓越中心，并将取代一个14岁的仪器，缺乏必要的能力，以满足研究人员的需求。 新的质谱卓越中心将取代目前的化学学院质谱设施，其中大多数工作人员运行的质谱仪无法满足现代生化和生物研究的需求。新中心将满足对最先进仪器的迫切需求，以满足其NIH资助的研究人员对非共价复合物分析和有限数量化学和生化化合物结构表征日益增长的需求。所提出的仪器将大大提高质量分析的速度和准确性，并将使少量的寡核苷酸，蛋白质，碳水化合物和生物无机复合物的准确质量分析成为可能。六名教员，每个人都将大大受益于内部，国家的最先进的电喷雾质谱仪的研究，进行了说明。其中五名研究人员已经建立了NIH资金，而一名研究人员过去曾成功获得NIH资金。这六个群体包括总共130名博士后研究人员，研究生和本科生，他们将直接受益于该设备。