QTOF MASS SPECTROMETER: CELL MOLECULAR BIOLOGY, PROTEIN STRUCTURE

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：加州大学伯克利分校化学学院和加州定量生物医学研究所（QB3）正在寻求资金购买一台配备电喷雾电离源的Q-TOF质谱仪。这台仪器将被安置在质谱卓越中心，并将取代一台有14年历史的仪器，该仪器缺乏满足研究人员需求所需的能力。新的质谱仪卓越中心将取代目前的化学学院质谱仪设施，在那里，大多数员工运行的质谱仪无法满足现代生化和生物学研究的需要。新中心将解决对最先进仪器的关键需求，以满足美国国立卫生研究院资助的研究人员对非共价复合物分析和有限数量化学和生化化合物的结构表征日益增长的需求。该仪器将显著提高质谱分析的速度和准确性，使少量寡核苷酸、蛋白质、碳水化合物和生物无机复合物的准确质谱分析成为可能。研究的六名教员，每个人都将受益于一个内部的，国家的最先进的电喷雾质谱仪，描述。这些研究人员中有5人已经建立了NIH资助，而1人过去曾成功获得NIH资助。这六个小组共包括130名博士后研究人员、研究生和本科生，他们将直接受益于该设备。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The University of California, Berkeley, College of Chemistry and the California Institute for Quantitative Biomedical Research (QB3) are seeking funds to purchase a Q-TOF mass spectrometer equipped with an electrospray ionization source. This instrument will be housed in the Mass Spectrometry Center of Excellence and will replace a 14-year-old instrument that lacks the capabilities necessary to serve researchers' needs. The new Mass Spectrometry Center of Excellence will replace the current College of Chemistry Mass Spectrometry Facility, where most of the staff-run mass spectrometers fail to meet the needs of modern biochemical and biological research. The new Center will address a critical need for state-of-the-art instrumentation to meet the growing demand for analysis of noncovalent complexes and structural characterization of limited amounts of chemical and biochemical compounds from its NIH-funded researchers. The proposed instrument will dramatically improve the speed and accuracy with which mass analysis can be done, and will make possible accurate mass analysis of small amounts of oligonucleotides, proteins, carbohydrates, and bio-inorganic complexes. The research of six faculty members, each of whom would benefit greatly from an in-house, state-of-the- art electrospray mass spectrometer, is described. Five of these researchers have established NIH funding, while one has successfully secured NIH funding in the past. These six groups include a total of 130 postdoctoral researchers, graduate students, and undergraduate students who will benefit directly from this equipment.