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High Definition Ion Mobility Spectrometer

高清晰度离子淌度谱仪

基本信息

批准号：
8826548
负责人：
Evan R Williams
金额：
$ 59.03万
依托单位：
UNIVERSITY OF CALIFORNIA BERKELEY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-05-01 至 2016-04-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Funds are requested to purchase a Waters Synapt G2-Si mass spectrometer that will provide new capabilities to researchers on the Berkeley campus, on other University of California campuses and other researchers outside of the UC system. This instrument will be part of the QB3/Chemistry Mass Spectrometry Facility with Profs. Williams, Healy, Portnoy, Rappaport, and Raymond as major users and Prof. Coates as a minor user of the instrument, and approximately 14 other research groups who would likely benefit from the capabilities of this instrument as well. The Mass Spectrometry Facility serves a local UC Berkeley community of about 75 chemistry and life sciences research laboratories. The requested instrument features ion mobility separation, which is currently not available in our facility or at any other laboratory on the Berkeley Campus. The Synapt will support a wide range of research activities focusing on separating isomers, isoforms, and isobaric compounds. When combined with high- performance tandem MS, ion mobility separations will allow us to differentiate samples by size, shape and charge as well as mass. Additionally the ion mobility feature of the Synapt appears to be well suited for the analysis of complex proteomics samples, i.e., proteolytic digests of complex protein mixtures. In particular, the unique ability of the ion mobility analyzer to separate peptide ions that overlap in the mass-to-charge ratio (m/z) dimension but which have different charge states produces dramatic improvements in overall proteome coverage. Project descriptions including preliminary from five major users and one minor user are provided to support our need for this instrument. This instrument will be housed in a QB3 Core Service Facility that will ensure its widest availability and most effective use in our research community. We are confident the addition of the Synapt G2-Si will significantly improve our ability to assist researchers within the life science community solve complicated research projects requiring advanced mass spectrometric equipment.

描述（由申请人提供）：资金要求购买沃茨Synapt G2-Si质谱仪，将提供新的能力，在伯克利分校的研究人员，在其他加州大学校园和其他研究人员以外的加州大学系统。该仪器将是QB 3/化学质谱设施的一部分，教授。威廉姆斯、希利、波特诺伊、拉帕波特和雷蒙德是该仪器的主要用户，科茨教授是该仪器的次要用户，还有大约14个其他研究小组也可能从该仪器的功能中受益。质谱设施服务于当地的加州大学伯克利分校社区约75个化学和生命科学研究实验室。所要求的仪器具有离子迁移率分离，这是目前在我们的设施或在伯克利校区的任何其他实验室不可用。Synapt将支持广泛的研究活动，重点是分离异构体，异构体和同量异序化合物。当与高性能串联质谱相结合时，离子迁移率分离将使我们能够通过大小，形状和电荷以及质量来区分样品。此外，Synapt的离子迁移率特征似乎非常适合分析复杂的蛋白质组学样本，即，复杂蛋白质混合物的蛋白水解酶。特别是离子的独特能力迁移率分析仪分离在质荷比（m/z）维度上重叠但具有不同电荷状态的肽离子，在总体蛋白质组覆盖率方面产生显著改善。项目描述，包括初步从五个主要用户和一个小用户提供，以支持我们需要这种仪器。该仪器将被安置在一个QB 3核心服务设施，这将确保其最广泛的可用性和最有效地使用在我们的研究社区。我们相信，Synapt G2-Si的加入将显著提高我们的能力，帮助生命科学界的研究人员解决需要先进质谱设备的复杂研究项目。

项目成果

期刊论文数量（13）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Identification of Glutaminyl Cyclase Genes Involved in Pyroglutamate Modification of Fungal Lignocellulolytic Enzymes.

DOI：
10.1128/mbio.02231-16
发表时间：
2017-01-17
期刊：
mBio
影响因子：
6.4
作者：
Wu VW;Dana CM;Iavarone AT;Clark DS;Glass NL
通讯作者：
Glass NL

Quick and facile preparation of histone proteins from the green microalga Chlamydomonas reinhardtii and other photosynthetic organisms

从绿色微藻莱茵衣藻和其他光合生物中快速简便地制备组蛋白

DOI：
10.1016/j.ymeth.2020.01.019
发表时间：
2020
期刊：
Methods
影响因子：
4.8
作者：
Wong, Amanda L.;Totah, Nicholas N.;Iavarone, Anthony T.;Pesavento, James J.
通讯作者：
Pesavento, James J.

Dissimilatory Sulfate Reduction Under High Pressure by Desulfovibrio alaskensis G20.

DOI：
10.3389/fmicb.2018.01465
发表时间：
2018
期刊：
Frontiers in microbiology
影响因子：
5.2
作者：
Williamson AJ;Carlson HK;Kuehl JV;Huang LL;Iavarone AT;Deutschbauer A;Coates JD
通讯作者：
Coates JD

Nitric Oxide-Induced Conformational Changes Govern H-NOX and Histidine Kinase Interaction and Regulation in Shewanella oneidensis.