Multiplexed Charge Detection Mass Spectrometer for Extended Mass and Collisional Cross Section Measurements
用于扩展质量和碰撞截面测量的多重电荷检测质谱仪
基本信息
- 批准号:10473780
- 负责人:
- 金额:$ 30.18万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-09-22 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:AreaBypassCell physiologyChargeComplexComplex MixturesConsumptionDataDetectionDevelopmentDissociationDrug TargetingElectrospray IonizationElectrostaticsEventFrequenciesGasesGenerationsGoalsHealthHeterogeneityHourHumanIndividualInjectionsIonsLipoproteinsMacromolecular ComplexesMass Spectrum AnalysisMeasurementMeasuresMethodsModelingMolecularOpticsParentsPathway interactionsPhasePolymersProcessProteinsPublishingResearchResolutionSamplingSchemeSignal TransductionSolventsSpeedSystemTechniquesTestingTherapeuticTherapeutic AgentsTimeViral VectorVirus-like particleWorkbasedesigndetectorevaporationexperienceexperimental studyimprovedinnovationinsightinstrumentinstrumentationion mobilitymacromolecular assemblymacromoleculemass spectrometermolecular assembly/self assemblymolecular shapenext generationnovelpreventprotein aggregationstemtargeted deliverytooltransmission processviral DNA
项目摘要
Project Summary
Large biomolecular assemblies constitute much of the molecular machinery necessary for
cellular function. These assemblies are often the target of a host of therapeutic molecules.
Other large biomolecular assemblies, including virus-like particles, proteinaceous cellular
compartments, and some large synthetic polymers, are potential delivery agents for these
therapeutics. The large molecules and molecular assemblies involved in cellular function can be
challenging to analyze using conventional mass spectrometry methods owing to their high mass
(>MDa) and heterogeneity. Electrospray ionization can transfer intact large
molecules/complexes into the gas phase, but overlapping and unresolved charge states that
stem from the heterogeneity inherent to high mass analytes often prevent mass measurements
using conventional mass spectrometers. Charge detection mass spectrometry weighs individual
ions, avoiding these interferences between ions and can be used to analyze molecules with
masses well above 100 MDa. However, this method can be slow because only one ion is
analyzed at a time. Here, a new generation of charge detection mass spectrometry is proposed
which provides information about the mass, the collisional cross section and the dissociation
pathways of individual ions of large macromolecular complexes, information that cannot be
obtained using conventional instruments. A key innovation is the development of multiplexing
methods that make it possible to simultaneously weigh many individual ions. These methods
have the potential to increase the speed of these measurements by up to 150x and reduce
sample analysis times to less than one minute. This ion multiplexing is aided by a novel
decoupling scheme whereby ions with a controlled range of energies are introduced into the
electrostatic ion detector trap so that two or more ions with the same m/z can have different
frequencies. The mass of each ion can be obtained from simultaneous measurements of the
individual ion frequency, energy, and charge. This scheme significantly reduces the potential for
overlapping ion signal by distributing the signal over a broad frequency bandwidth. The rate of
ion energy change can also be determined from these measurements, providing information
about collisional cross sections and giving insight into molecular shape. Similarly, individual ion
fragmentation events can be tracked and used to obtain additional structural information.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Evan R Williams其他文献
Combined Multiharmonic Frequency Analysis for Improved Dynamic Energy Measurements and Accuracy in Charge Detection Mass Spectrometry.
组合多谐波频率分析可提高动态能量测量和电荷检测质谱的准确性。
- DOI:
- 发表时间:
2023 - 期刊:
- 影响因子:7.4
- 作者:
Conner C Harper;Zachary M. Miller;Evan R Williams - 通讯作者:
Evan R Williams
Evan R Williams的其他文献
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{{ truncateString('Evan R Williams', 18)}}的其他基金
Multiplexed Charge Detection Mass Spectrometer for Extended Mass and Collisional Cross Section Measurements
用于扩展质量和碰撞截面测量的多重电荷检测质谱仪
- 批准号:
10267735 - 财政年份:2020
- 资助金额:
$ 30.18万 - 项目类别:
Integrated Methods for Structural Elucidation of Proteins and Macromolecular Comp
蛋白质和大分子化合物结构解析的综合方法
- 批准号:
8297329 - 财政年份:2012
- 资助金额:
$ 30.18万 - 项目类别:
Integrated Methods for Structural Elucidation of Proteins and Macromolecular Comp
蛋白质和大分子化合物结构解析的综合方法
- 批准号:
8641395 - 财政年份:2012
- 资助金额:
$ 30.18万 - 项目类别:
Integrated Methods for Structural Elucidation of Proteins and Macromolecular Comp
蛋白质和大分子化合物结构解析的综合方法
- 批准号:
8828711 - 财政年份:2012
- 资助金额:
$ 30.18万 - 项目类别:
Integrated Methods for Structural Elucidation of Proteins and Macromolecular Comp
蛋白质和大分子化合物结构解析的综合方法
- 批准号:
8442272 - 财政年份:2012
- 资助金额:
$ 30.18万 - 项目类别:
Development of Single Particle Analyzer of Mass and Mobility (SPAMM)
单粒子质量和迁移率分析仪(SPAMM)的开发
- 批准号:
8499373 - 财政年份:2011
- 资助金额:
$ 30.18万 - 项目类别:
Development of Single Particle Analyzer of Mass and Mobility (SPAMM)
单粒子质量和迁移率分析仪(SPAMM)的开发
- 批准号:
8686003 - 财政年份:2011
- 资助金额:
$ 30.18万 - 项目类别:
Development of Single Particle Analyzer of Mass and Mobility (SPAMM)
单粒子质量和迁移率分析仪(SPAMM)的开发
- 批准号:
8026269 - 财政年份:2011
- 资助金额:
$ 30.18万 - 项目类别:
Development of Single Particle Analyzer of Mass and Mobility (SPAMM)
单粒子质量和迁移率分析仪(SPAMM)的开发
- 批准号:
8290324 - 财政年份:2011
- 资助金额:
$ 30.18万 - 项目类别:
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