IN VIVO MICROCT IMAGING AND ANALYSIS SYSTEM FOR RODENTS: OSTEOPOROSIS

啮齿动物体内显微成像和分析系统：骨质疏松症

• 批准号: 7335122
• 负责人: DOUGLAS J ADAMS
• 金额: $ 6.98万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335122
• 关键词: aging; bone; health; medicine; model; osteoporosis; tissues; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This application is to obtain funding for the purchase of a high resolution in vivo micro-computed tomography (microCT) imaging system for use with rodent models at the University of Connecticut Health Center. This instrument, the Scanco VivaCT40, will become part of an active MicroCT Facility. The core facility provides services to investigators from several departments within the Schools of Medicine and Dental Medicine, with a primary research emphasis in bone biology and combined annual NIH funding of $6.5 million. The facility also provides core services to investigators from Yale University and the University of Pennsylvania. Although our present microCT imaging capability has proven to be beneficial to a large number of investigators, it is limited to analysis of excised tissue samples, precluding longitudinal studies and requiring large numbers of animals for analysis at multiple time points. In vivo microCT imaging and analysis will be helpful in studies involving transgenic mouse models, allowing phenotypic quantitation of skeletal elements during maturation and aging. Likewise, current studies involving contrast-enhanced imaging of organs, tumors, and vascular structures will benefit greatly with the capability of high-resolution in vivo CT imaging and real-time perfusion of contrast media. Because ex vivo microCT imaging and morphometric analysis of excised tissue structures is nondestructive and rapid, our existing system will continue to provide essential data from specimens harvested for histomorphometric examination, gene expression assays, and mechanical integrity tests. Moreover, because the resolution of the in vivo and ex vivo systems are equivalent and analysis software identical, the Alphastations used to operate the instruments can be clustered together. The resulting increase in computational capacity will allow us to scan at the highest resolution available. Relevance to public health: The investigators who will use this instrument have developed murine models that are highly relevant to our understanding of the mechanisms of bone loss with aging, estrogen deficiency, and immobilization. The increase in the quality and quantity of results that will be obtained with an in vivo microCT system will make it possible to develop new approaches to the maintenance of bone health and treatment of bone disease more rapidly. This has been recognized as a critical issue by the Surgeon General's Report on "Bone Health and Osteoporosis".

该副本是利用NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是赠款，这不一定是调查人员的机构。描述（由申请人提供）：此申请是为了购买高分辨率在康涅狄格大学健康中心的啮齿动物模型中使用的高分辨率中的资金。该仪器是Scanco Vivact40，将成为活动微型CTIONT的一部分。核心设施为医学和牙科医学学校多个部门的调查人员提供服务，主要研究重点是骨骼生物学，并将年度NIH资金合并为650万美元。该设施还为耶鲁大学和宾夕法尼亚大学的调查人员提供核心服务。尽管我们目前的MicroCT成像能力已被证明对大量研究人员有益，但它仅限于分析切除的组织样品，排除纵向研究，并需要大量动物在多个时间点进行分析。体内MicroCT成像和分析将有助于涉及转基因小鼠模型的研究，从而可以在成熟和衰老过程中对骨骼元素进行表型定量。同样，当前涉及器官，肿瘤和血管结构的对比增强成像的研究将在体内成像中高分辨率的能力以及对比造影介质的实时灌注的能力受益匪浅。由于切除的组织结构的离体微观成像和形态计量分析是无损和快速的，因此我们现有的系统将继续从收获的标本中提供基本数据，用于组织形态计量学检查，基因表达测定和机械完整性测试。此外，由于体内和体内系统的分辨率是等效的，并且分析软件相同，因此用于操作仪器的字母可以聚集在一起。由此产生的计算能力增加将使我们能够在可用的最高分辨率下扫描。与公共卫生的相关性：将使用该工具的研究人员开发了与我们对衰老，雌激素缺乏和固定化的骨质流失机制高度相关的鼠模型。通过体内Microct系统将获得的结果的质量和数量的增加将使您可以更快地开发维持骨骼健康和治疗骨骼疾病的新方法。外科医生关于“骨骼健康和骨质疏松症”的报告已将其视为关键问题。