Nanomechanical Test System for Small Length Scale Biomaterials & Tissue Specimens

小尺寸生物材料纳米力学测试系统

• 批准号: 7796480
• 负责人: DOUGLAS J ADAMS
• 金额: $ 35.88万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-13 至 2011-05-12
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7796480
• 关键词: Animal Model; Area; Behavior; Biocompatible Materials; Biological; Biological Assay; Bone Resorption; Cartilage; Cell Culture Techniques; Characteristics; Clinical Data; Core Facility; Data; Development; Dimensions; Disease; Engineering; Equipment; Extracellular Matrix; Fee-for-Service Plans; Fracture; Image; Injury; International; Joints; Laboratories; Length; Measurement; Measures; Mechanics; Methods; Modeling; Molecular; Mus; Musculoskeletal; Natural regeneration; Optics; Osteoclasts; Physics; Process; Property; Publications; Research Personnel; Services; Solutions; Specimen; Structure; Surface; System; Techniques; Testing; Tissue Engineering; Tissues; Tooth structure; Wound Healing; animal tissue; base; biomaterial development; biomaterial interface; bone; genetic manipulation; human disease; improved; instrument; investigator training; nanomechanical; nanometer; novel; pre-clinical; regenerative; repaired; scaffold; soft tissue

DESCRIPTION (provided by applicant): The objectives of the projects outlined in this shared equipment application are broadly defined to provide small-scale mechanical testing data for a large group of NIH-sponsored investigators with expertise in areas of biomaterials development for tissue engineering applications, analysis of tissue repair and regeneration, and molecular studies of bone and joint tissue disorders. The long-term aims are: Aim 1: Establish expertise in nanomechanical testing within the UCHC Micromechanics Core Faciltiy as an additional service to UCHC and regional investigators. Aim 2: Educate and train investigators to use nanomechanical test instruments, with additional focus on investigators in discplines outside of physics and engineering (i.e., biologists). Aim 3: Provide nanomechanical testing on a fee-for-service basis, utilizing the management and operational model of our well-established Micro-CT Imaging & Micromechanics core facility. Aim 4: Disseminate findings and new techniques through publication and presentation of results to national and international audiences. Nanomechanical testing will be used: 1) to measure properties of biomaterials and coatings at minute length scales, allowing direct tests of tissue engineering scaffolds with characteristic dimensions as small as micrometers or tens of nanometers. This will allow initial characterization of material integrity to test hypotheses for improving material behavior toward that of biological extracellular matrix; 2) to examine the integrity of repair and regenerative tissues, including tissue-biomaterial interfaces; 3) for phenotypic mechanical property measurement of bone, tooth, cartilage, and other soft tissue structures obtained from mice with unique genetic manipulations useful for studying human disease processes; 4) to develop novel laboratory benchtop tests to improve existing assays, such as scratch-fracture tests of murine joint cartilage and the optical profilometry approach we describe in the application for measuring volumetric bone resorption rates in osteoclast cell cultures. Collectively, these methods provide a regimented solution to mechanical surface testing of small length scale constructs associated with animal tissues, tissue engineering development, and tissue repair in small animal models. The data provided by nanometer and micrometer scale mechanical tests is paramount in these studies, allowing for meaningful acquisition and interpretation of pre-clinical data in studies aimed at treating musculoskeletal tissue injuries and disorders.

描述（由申请人提供）：本共享设备申请中概述的项目的目的是广泛定义的，为大量NIH申办的研究者提供小规模机械试验数据，这些研究者在组织工程应用的生物材料开发、组织修复和再生分析以及骨和关节组织疾病的分子研究领域具有专业知识。长期目标是：目标1：在UCHC微机械核心设施内建立纳米机械测试专业知识，作为UCHC和区域调查人员的额外服务。目标二：教育和培训研究人员使用纳米机械测试仪器，并额外关注物理和工程以外学科的研究人员（即，生物学家）。目标3：利用我们完善的Micro-CT成像和微力学核心设施的管理和运营模式，提供按服务收费的纳米力学测试。目标4：通过出版物和向国家和国际受众介绍结果，传播研究结果和新技术。纳米力学测试将用于：1）以微小的长度尺度测量生物材料和涂层的特性，允许直接测试特征尺寸小至微米或数十纳米的组织工程支架。这将允许对材料完整性进行初步表征，以测试改善材料行为的假设，使其接近生物细胞外基质; 2）检查修复和再生组织的完整性，包括组织-生物材料界面; 3）用于骨、牙齿、软骨的表型机械性能测量，和其他软组织结构，这些软组织结构通过独特的遗传操作从小鼠获得，可用于研究人类疾病过程; 4）开发新的实验室台式测试以改进现有的测定，例如鼠关节软骨的划痕-断裂测试和我们在用于测量破骨细胞培养物中的体积骨吸收率的应用中描述的光学轮廓测定法。总的来说，这些方法为与动物组织、组织工程开发和小动物模型中的组织修复相关的小长度尺度结构的机械表面测试提供了有组织的解决方案。在这些研究中，纳米和微米尺度力学测试提供的数据至关重要，可以在旨在治疗肌肉骨骼组织损伤和疾病的研究中对临床前数据进行有意义的采集和解释。