Nanomechanical Test System for Small Length Scale Biomaterials & Tissue Specimens

小尺寸生物材料纳米力学测试系统

• 批准号: 7796480
• 负责人: DOUGLAS J ADAMS
• 金额: $ 35.88万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-13 至 2011-05-12
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7796480
• 关键词: Animal Model; Area; Behavior; Biocompatible Materials; Biological; Biological Assay; Bone Resorption; Cartilage; Cell Culture Techniques; Characteristics; Clinical Data; Core Facility; Data; Development; Dimensions; Disease; Engineering; Equipment; Extracellular Matrix; Fee-for-Service Plans; Fracture; Image; Injury; International; Joints; Laboratories; Length; Measurement; Measures; Mechanics; Methods; Modeling; Molecular; Mus; Musculoskeletal; Natural regeneration; Optics; Osteoclasts; Physics; Process; Property; Publications; Research Personnel; Services; Solutions; Specimen; Structure; Surface; System; Techniques; Testing; Tissue Engineering; Tissues; Tooth structure; Wound Healing; animal tissue; base; biomaterial development; biomaterial interface; bone; genetic manipulation; human disease; improved; instrument; investigator training; nanomechanical; nanometer; novel; pre-clinical; regenerative; repaired; scaffold; soft tissue

DESCRIPTION (provided by applicant): The objectives of the projects outlined in this shared equipment application are broadly defined to provide small-scale mechanical testing data for a large group of NIH-sponsored investigators with expertise in areas of biomaterials development for tissue engineering applications, analysis of tissue repair and regeneration, and molecular studies of bone and joint tissue disorders. The long-term aims are: Aim 1: Establish expertise in nanomechanical testing within the UCHC Micromechanics Core Faciltiy as an additional service to UCHC and regional investigators. Aim 2: Educate and train investigators to use nanomechanical test instruments, with additional focus on investigators in discplines outside of physics and engineering (i.e., biologists). Aim 3: Provide nanomechanical testing on a fee-for-service basis, utilizing the management and operational model of our well-established Micro-CT Imaging & Micromechanics core facility. Aim 4: Disseminate findings and new techniques through publication and presentation of results to national and international audiences. Nanomechanical testing will be used: 1) to measure properties of biomaterials and coatings at minute length scales, allowing direct tests of tissue engineering scaffolds with characteristic dimensions as small as micrometers or tens of nanometers. This will allow initial characterization of material integrity to test hypotheses for improving material behavior toward that of biological extracellular matrix; 2) to examine the integrity of repair and regenerative tissues, including tissue-biomaterial interfaces; 3) for phenotypic mechanical property measurement of bone, tooth, cartilage, and other soft tissue structures obtained from mice with unique genetic manipulations useful for studying human disease processes; 4) to develop novel laboratory benchtop tests to improve existing assays, such as scratch-fracture tests of murine joint cartilage and the optical profilometry approach we describe in the application for measuring volumetric bone resorption rates in osteoclast cell cultures. Collectively, these methods provide a regimented solution to mechanical surface testing of small length scale constructs associated with animal tissues, tissue engineering development, and tissue repair in small animal models. The data provided by nanometer and micrometer scale mechanical tests is paramount in these studies, allowing for meaningful acquisition and interpretation of pre-clinical data in studies aimed at treating musculoskeletal tissue injuries and disorders.

描述（由申请人提供）：此共享设备申请中概述的项目目标广泛定义为为 NIH 资助的一大群研究人员提供小规模机械测试数据，这些研究人员在组织工程应用的生物材料开发、组织修复和再生分析以及骨和关节组织疾病的分子研究等领域具有专业知识。长期目标是： 目标 1：在 UCHC 微力学核心设施内建立纳米力学测试专业知识，作为 UCHC 和区域研究人员的附加服务。目标 2：教育和培训研究人员使用纳米力学测试仪器，特别关注物理和工程学以外学科的研究人员（即生物学家）。目标 3：利用我们完善的 Micro-CT 成像和微机械核心设施的管理和运营模型，以收费服务为基础提供纳米机械测试。目标 4：通过向国内和国际受众出版和展示结果来传播研究结果和新技术。纳米力学测试将用于：1）在微小长度尺度上测量生物材料和涂层的性能，从而可以直接测试特征尺寸小至微米或数十纳米的组织工程支架。这将允许对材料完整性进行初步表征，以测试针对生物细胞外基质改善材料行为的假设； 2）检查修复和再生组织的完整性，包括组织-生物材料界面； 3) 通过独特的基因操作对小鼠的骨、牙齿、软骨和其他软组织结构进行表型机械特性测量，有助于研究人类疾病过程； 4）开发新颖的实验室台式测试以改进现有的测定，例如小鼠关节软骨的划痕骨折测试和我们在应用中描述的用于测量破骨细胞培养物中体积骨吸收率的光学轮廓测定方法。总的来说，这些方法为与动物组织、组织工程开发和小动物模型中的组织修复相关的小长度结构的机械表面测试提供了严格的解决方案。纳米和微米级机械测试提供的数据在这些研究中至关重要，可以在旨在治疗肌肉骨骼组织损伤和疾病的研究中对临床前数据进行有意义的获取和解释。