Nanomechanical Test System for Small Length Scale Biomaterials & Tissue Specimens

小尺寸生物材料纳米力学测试系统

• 批准号: 7796480
• 负责人: DOUGLAS J ADAMS
• 金额: $ 35.88万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-13 至 2011-05-12
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7796480
• 关键词: Animal Model; Area; Behavior; Biocompatible Materials; Biological; Biological Assay; Bone Resorption; Cartilage; Cell Culture Techniques; Characteristics; Clinical Data; Core Facility; Data; Development; Dimensions; Disease; Engineering; Equipment; Extracellular Matrix; Fee-for-Service Plans; Fracture; Image; Injury; International; Joints; Laboratories; Length; Measurement; Measures; Mechanics; Methods; Modeling; Molecular; Mus; Musculoskeletal; Natural regeneration; Optics; Osteoclasts; Physics; Process; Property; Publications; Research Personnel; Services; Solutions; Specimen; Structure; Surface; System; Techniques; Testing; Tissue Engineering; Tissues; Tooth structure; Wound Healing; animal tissue; base; biomaterial development; biomaterial interface; bone; genetic manipulation; human disease; improved; instrument; investigator training; nanomechanical; nanometer; novel; pre-clinical; regenerative; repaired; scaffold; soft tissue

DESCRIPTION (provided by applicant): The objectives of the projects outlined in this shared equipment application are broadly defined to provide small-scale mechanical testing data for a large group of NIH-sponsored investigators with expertise in areas of biomaterials development for tissue engineering applications, analysis of tissue repair and regeneration, and molecular studies of bone and joint tissue disorders. The long-term aims are: Aim 1: Establish expertise in nanomechanical testing within the UCHC Micromechanics Core Faciltiy as an additional service to UCHC and regional investigators. Aim 2: Educate and train investigators to use nanomechanical test instruments, with additional focus on investigators in discplines outside of physics and engineering (i.e., biologists). Aim 3: Provide nanomechanical testing on a fee-for-service basis, utilizing the management and operational model of our well-established Micro-CT Imaging & Micromechanics core facility. Aim 4: Disseminate findings and new techniques through publication and presentation of results to national and international audiences. Nanomechanical testing will be used: 1) to measure properties of biomaterials and coatings at minute length scales, allowing direct tests of tissue engineering scaffolds with characteristic dimensions as small as micrometers or tens of nanometers. This will allow initial characterization of material integrity to test hypotheses for improving material behavior toward that of biological extracellular matrix; 2) to examine the integrity of repair and regenerative tissues, including tissue-biomaterial interfaces; 3) for phenotypic mechanical property measurement of bone, tooth, cartilage, and other soft tissue structures obtained from mice with unique genetic manipulations useful for studying human disease processes; 4) to develop novel laboratory benchtop tests to improve existing assays, such as scratch-fracture tests of murine joint cartilage and the optical profilometry approach we describe in the application for measuring volumetric bone resorption rates in osteoclast cell cultures. Collectively, these methods provide a regimented solution to mechanical surface testing of small length scale constructs associated with animal tissues, tissue engineering development, and tissue repair in small animal models. The data provided by nanometer and micrometer scale mechanical tests is paramount in these studies, allowing for meaningful acquisition and interpretation of pre-clinical data in studies aimed at treating musculoskeletal tissue injuries and disorders.

描述（由申请人提供）：该共享设备应用程序中概述的项目的目标是广泛定义的，是为大量的NIH赞助的研究人员提供小型机械测试数据，这些研究人员在组织工程应用的生物材料开发领域具有专业知识，分析组织修复和再生和再生以及骨骼和关节组织疾病的分子研究。长期的目标是：目标1：在UCHC微力学核心方面的纳米力学测试方面建立专业知识，作为UCHC和区域研究人员的额外服务。 AIM 2：教育和培训研究人员使用纳米力学测试仪器，额外的重点是物理和工程外（即生物学家）以外的疾病中的调查员。 AIM 3：利用我们已建立的Micro-CT成像和微力学核心设施的管理和操作模型，以支付费用提供纳米力学测试。目标4：通过向国家和国际观众发表和呈现结果来传播发现和新技术。将使用纳米力学测试：1）在微小尺度上测量生物材料和涂料的性能，从而直接测试具有特征性尺寸的较小或数十纳米米的特征性尺寸的组织工程支架。这将允许对材料完整性的初始表征来检验假设，以改善材料行为对生物细胞外基质的行为； 2）检查修复和再生组织的完整性，包括组织生物材料界面； 3）用于对骨，牙齿，软骨和其他软组织结构的表型机械性能测量，这些结构从小鼠获得，具有独特的遗传操作，可用于研究人类病过程； 4）开发新的实验室台式测试以改善现有测定法，例如鼠关节软骨的刮擦裂缝测试以及我们在测量骨质细胞细胞培养物中体积骨吸收速率的应用中描述的光学传递方法。总的来说，这些方法为与动物组织，组织工程发展和小动物模型中的小规模构建体的机械表面测试提供了一种有条理的解决方案。在这些研究中，纳米和微米尺度机械测试提供的数据至关重要，从而使旨在治疗肌肉骨骼组织损伤和疾病的研究中有意义地获取和解释临床前数据。