Response Of Renal Cells To Osmotic Stress

肾内髓质血液渗透压高，随尿液浓度变化而变化。氯化钠和尿素都升高了。显然，骨髓细胞在这种不利的环境中存活并发挥作用。正在进行的研究关注于了解所涉及的机制。当细胞受到高盐环境的胁迫时，它们通常会积累具有渗透活性的有机溶质(渗透压物质)，以维持良好的内部环境，同时调节其体积。我们鉴定了肾内髓细胞中的有机渗透物质为甘油磷胆碱(GPC)、甜菜碱、山梨醇、牛磺酸和肌醇，并表明渗透物质水平随尿液浓度(可能还有髓盐和尿素浓度)而变化。我们现在正在使用肾细胞培养和活体动物来研究这些有机渗透调节物质的渗透调节积累的机制和控制，包括涉及的转录因子(TONEBP/OREBP)。高盐通过PKA、ATM、PARP-1、HSP90、PI3K等系统激活TONEBP。此外，对高渗透压的急性反应可能包括肾髓质细胞增殖培养中的细胞周期停滞和凋亡。高盐会导致细胞培养中DNA的损伤，而高尿素会导致DNA(8-氧鸟嘌呤损伤)和蛋白质(羧化)损伤。正常肾内髓既有DNA损伤，又有蛋白质羰化。当利尿剂速尿降低肾内髓质渗透压时，DNA损伤可在体内迅速修复。我们正在研究相关的机制，包括P53、p38、Ku86和GADD蛋白的作用。

The osmolality of the blood in the renal inner medulla is high and varies with the urinary concentration. Both NaCl and urea are elevated. The medullary cells evidently survive and function in this adverse environment. The ongoing studies are concerned with understanding the mechanisms involved. When cells are stressed by a high salt environment, they generally accumulate osmotically active organic solutes ("osmolytes") in order to maintain a favorable internal milieu, while regulating their volume. We identified the organic osmolytes in renal inner medullary cells as glycerophosphocholine (GPC), betaine, sorbitol, taurine, and inositol, and showed that the osmolyte levels varied with urine concentration (and, presumably, medullary salt and urea concentrations). We are now using renal cell cultures and living animals to study the mechanism and control of osmoregulatory accumulation of these organic osmolytes, including the transcription factor (TonEBP/OREBP) that is involved. High NaCl signals activation of TonEBP via PKA, ATM, PARP-1, HSP90, PI3K and other systems. In addition, the acute response to high osmolality may include cell cycle arrest and apoptosis in proliferating cultures of renal medullary cells. High NaCl causes DNA damage in cell culture, while high urea causes both DNA (8-oxoguanine lesions) and protein(cabonylation) damage. Both the DNA damage and protein carbonylation are present in normal renal inner medullas. The DNA damage is rapidly repaired in vivo when renal inner medullary osmolality is reduced by the diuretic, furosemide. We are studying the mechanisms involved, including the role of p53, p38, Ku86, and GADD proteins.