Verification of the Structures of Bacterial Polysacchari

通过一种或多种核磁共振方法对目标多糖进行了分析，包括用于1H谱归属的1D 1H核磁共振、2D相关谱(COSY)和2D全相关谱(TOCSY)，以及用于13C核磁共振谱解析的1D 13CNMR、1H偶联和解偶联、2D异核单量子相关(HSQC)和2D异核多键相关(HMBC)。 脑膜炎奈瑟氏菌W135：用核磁共振氢谱分析了W135 CPS中天然和去O-乙酰化的荚膜多糖的N-乙酰和O-乙酰含量。7-O-乙酰基和9-O-乙酰基-N-乙酰神经氨酸的N-乙酰甲基和O-乙酰甲基信号的定量积分表明，CPS的总O-乙酰基含量为1.1-1.2摩尔%，接近光谱仪的检出限。 流感嗜血杆菌A：用1D 1H核磁共振、1D 13C核磁共振、2D COSY、2D TOCSY、1H偶联和解偶联2D HSQC、2D HMBC对纯化前后的CPS进行了分析。CPS的聚磷酸盐结构的最强证据是在13C核磁共振谱上，它在适当的13C共振中显示了5个13C-31P偶联常数。核磁共振谱也表明制备的产物纯度很高。

The polysaccharides of interest have been analyzed by one or more of a variety of NMR methods, including 1D 1H NMR, 2D correlation spectroscopy (COSY), and 2D total correlation spectroscopy (TOCSY) for assignment of 1H spectra, and 1D 13C NMR, 1H coupled and decoupled, 2D heteronuclear single quantum correlation (HSQC), and 2D heteronuclear multiple bond correlation (HMBC) for the elucidation of 13C NMR spectra. NEISSERIA MENINGITIDIS W135: Preparations of native and de-O-acetylated capsular polysaccharide from W135 CPS were analyzed for N-acetyl and O-acetyl content by 1H NMR spectroscopy. Quantitative integration of the N-acetyl and O-acetyl methyl signals of the 7-O-acetyl- and 9-O-acetyl-N-acetylneuraminic acid components indicated that the total O-acetyl content of the CPS was 1.1-1.2 mole%, i.e., close to the detection limit of the spectrometer. HAEMOPHILUS INFLUENZAE A: Preparations of the native and purified CPS were analyzed by 1D 1H NMR, 1D 13C NMR, 2D COSY, 2D TOCSY, 1H coupled and decoupled 2D HSQC, and 2D HMBC. The strongest evidence for the polyphosphate structure of the CPS was apparent in the 13C NMR spectrum, which displayed five 13C-31P coupling constants in the appropriate 13C resonances. NMR also indicated that the purity of the preparations was excellent.