Verification of the Structures of Bacterial Polysacchari

已经通过多种NMR方法中的一种或多种分析了感兴趣的多糖，所述NMR方法包括1D 1H NMR、2D相关光谱（COSY）和用于1H光谱归属的2D总相关光谱（TOCSY），以及1D 13 C NMR、1H耦合和去耦合、2D异质单量子相关（HSQC）、和2D异核多键相关（HMBC）用于阐明13 C NMR光谱。 脑膜炎奈瑟氏球菌W135：通过1H NMR光谱分析来自W135 CPS的天然和脱-O-乙酰化荚膜多糖制剂的N-乙酰基和O-乙酰基含量。7-O-乙酰基-和9-O-乙酰基-N-乙酰基神经氨酸组分的N-乙酰基和O-乙酰基甲基信号的定量积分表明CPS的总O-乙酰基含量为1.1-1.2摩尔%，即，接近光谱仪的检测极限。 嗜血嗜血杆菌A：通过1D 1H NMR、1D 13 C NMR、2D COSY、2D TOCSY、1H偶联和去偶联的2D HSQC和2D HMBC分析天然和纯化的CPS的制备物。CPS多磷酸盐结构的最强证据在13 C NMR谱中显而易见，该谱在适当的13 C共振中显示了5个13 C-31 P耦合常数。NMR也表明制备物的纯度是极好的。

The polysaccharides of interest have been analyzed by one or more of a variety of NMR methods, including 1D 1H NMR, 2D correlation spectroscopy (COSY), and 2D total correlation spectroscopy (TOCSY) for assignment of 1H spectra, and 1D 13C NMR, 1H coupled and decoupled, 2D heteronuclear single quantum correlation (HSQC), and 2D heteronuclear multiple bond correlation (HMBC) for the elucidation of 13C NMR spectra. NEISSERIA MENINGITIDIS W135: Preparations of native and de-O-acetylated capsular polysaccharide from W135 CPS were analyzed for N-acetyl and O-acetyl content by 1H NMR spectroscopy. Quantitative integration of the N-acetyl and O-acetyl methyl signals of the 7-O-acetyl- and 9-O-acetyl-N-acetylneuraminic acid components indicated that the total O-acetyl content of the CPS was 1.1-1.2 mole%, i.e., close to the detection limit of the spectrometer. HAEMOPHILUS INFLUENZAE A: Preparations of the native and purified CPS were analyzed by 1D 1H NMR, 1D 13C NMR, 2D COSY, 2D TOCSY, 1H coupled and decoupled 2D HSQC, and 2D HMBC. The strongest evidence for the polyphosphate structure of the CPS was apparent in the 13C NMR spectrum, which displayed five 13C-31P coupling constants in the appropriate 13C resonances. NMR also indicated that the purity of the preparations was excellent.