Verification of the Structures of Bacterial Polysacchari

研究人员利用一种或多种核磁共振方法对多糖进行了分析，其中包括1H NMR、2D相关光谱（COSY）和2D全相关光谱（TOCSY），用于1H谱的赋值；13C NMR、1H耦合和解耦、2D异核单量子相关（HSQC）和2D异核多键相关（HMBC）用于13C谱的解析。

The polysaccharides of interest have been analyzed by one or more of a variety of NMR methods, including 1D 1H NMR, 2D correlation spectroscopy (COSY), and 2D total correlation spectroscopy (TOCSY) for assignment of 1H spectra, and 1D 13C NMR, 1H coupled and decoupled, 2D heteronuclear single quantum correlation (HSQC), and 2D heteronuclear multiple bond correlation (HMBC) for the elucidation of 13C NMR spectra. NEISSERIA MENINGITIDIS W135: Preparations of native and de-O-acetylated capsular polysaccharide from W135 CPS were analyzed for N-acetyl and O-acetyl content by 1H NMR spectroscopy. Quantitative integration of the N-acetyl and O-acetyl methyl signals of the 7-O-acetyl- and 9-O-acetyl-N-acetylneuraminic acid components indicated that the total O-acetyl content of the CPS was 1.1-1.2 mole%, i.e., close to the detection limit of the spectrometer. HAEMOPHILUS INFLUENZAE A: Preparations of the native and purified CPS were analyzed by 1D 1H NMR, 1D 13C NMR, 2D COSY, 2D TOCSY, 1H coupled and decoupled 2D HSQC, and 2D HMBC. The strongest evidence for the polyphosphate structure of the CPS was apparent in the 13C NMR spectrum, which displayed five 13C-31P coupling constants in the appropriate 13C resonances. NMR also indicated that the purity of the preparations was excellent.