Training in Animal Cardiac Transplant Surgical Procedure

动物心脏移植手术培训

• 批准号: 7332556
• 负责人: michael a solomon
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7332556
• 关键词: Training; Animal; Cardiac; Transplant; Surgical

This protocol (CCM 0404) is part of an overall program to study acute cellular rejection, immunotolerance and cardiac allograft vasculopathy in a rat cardiac transplant model. The rodent model of heterotopic cardiac transplantation is a well established model in the field of solid organ transplantation. The purpose of this protocol is to make provisions to train personnel in the rat cardiac transplant surgical procedure. The anticipated outcome is to master the technical skills prior to the initiation of an animal study, thereby minimizing unnecessary animal suffering and loss due to research procedures. The protocol also allows for the harvesting of organs after euthanizing the animal for the purposes of: histopathological review; training individuals in the sample processing techniques; and providing means for experienced laboratory personnel to develop improved sample processing techniques. There were 40 animals used during the ACUC reporting period, with a total of 270 used and 3 investigators trained since the protcol was approved.

本方案（CCM 0404）是在大鼠心脏移植模型中研究急性细胞排斥反应、免疫耐受和心脏移植物血管病变的整体计划的一部分。啮齿动物异位心脏移植模型是实体器官移植领域中建立良好的模型。本方案旨在提供大鼠心脏移植手术中的人员培训。预期的结果是在开始动物研究之前掌握技术技能，从而最大限度地减少研究程序导致的不必要的动物痛苦和损失。该方案还允许在对动物实施安乐死后收获器官，用于：组织病理学审查;对个体进行样本处理技术培训;以及为经验丰富的实验室人员提供开发改进的样本处理技术的方法。ACUC报告期间使用了40只动物，自方案获批以来，共使用了270只动物，3名研究者接受了培训。