Training in Animal Cardiac Transplant Surgical Procedure

该方案(CCM 0404)是研究大鼠心脏移植模型中急性细胞排斥、免疫耐受和心脏移植物血管病变的整体计划的一部分。异位心脏移植啮齿动物模型是实体器官移植领域较为成熟的动物模型。该议定书的目的是为培训大鼠心脏移植手术程序的人员做好准备。预计的结果是在开始动物研究之前掌握技术技能，从而将研究过程中不必要的动物痛苦和损失降至最低。该议定书还允许在对动物实施安乐死后采集器官，目的是：组织病理学审查；对个人进行样本处理技术培训；以及为有经验的实验室人员提供手段，以开发改进的样本处理技术。在ACUC报告所述期间，使用了40只动物，总共使用了270只，自协议获得批准以来，有3名调查人员接受了培训。

This protocol (CCM 0404) is part of an overall program to study acute cellular rejection, immunotolerance and cardiac allograft vasculopathy in a rat cardiac transplant model. The rodent model of heterotopic cardiac transplantation is a well established model in the field of solid organ transplantation. The purpose of this protocol is to make provisions to train personnel in the rat cardiac transplant surgical procedure. The anticipated outcome is to master the technical skills prior to the initiation of an animal study, thereby minimizing unnecessary animal suffering and loss due to research procedures. The protocol also allows for the harvesting of organs after euthanizing the animal for the purposes of: histopathological review; training individuals in the sample processing techniques; and providing means for experienced laboratory personnel to develop improved sample processing techniques. There were 40 animals used during the ACUC reporting period, with a total of 270 used and 3 investigators trained since the protcol was approved.