Training in Animal Cardiac Transplant Surgical Procedure

该方案（CCM 0404）是在大鼠心脏移植模型中研究急性细胞排斥反应、免疫耐受和同种异体心脏移植血管病变的整体方案的一部分。啮齿动物异位心脏移植模型是实体器官移植领域中较为成熟的模型。本协议的目的是对大鼠心脏移植手术过程中的人员进行培训。预期的结果是在开始动物研究之前掌握技术技能，从而最大限度地减少由于研究程序造成的不必要的动物痛苦和损失。该协议还允许在对动物实施安乐死后采集器官，用于：组织病理学检查；对个人进行样品处理技术培训；并为经验丰富的实验室人员提供开发改进的样品处理技术的手段。在ACUC报告期间共使用了80只动物，自方案批准以来共使用了350只动物，并培训了3名调查员。

This protocol (CCM 0404) is part of an overall program to study acute cellular rejection, immunotolerance and cardiac allograft vasculopathy in a rat cardiac transplant model. The rodent model of heterotopic cardiac transplantation is a well established model in the field of solid organ transplantation. The purpose of this protocol is to make provisions to train personnel in the rat cardiac transplant surgical procedure. The anticipated outcome is to master the technical skills prior to the initiation of an animal study, thereby minimizing unnecessary animal suffering and loss due to research procedures. The protocol also allows for the harvesting of organs after euthanizing the animal for the purposes of: histopathological review; training individuals in the sample processing techniques; and providing means for experienced laboratory personnel to develop improved sample processing techniques. There were 80 animals used during the ACUC reporting period, with a total of 350 used and 3 investigators trained since the protocol was approved.