Training in Animal Cardiac Transplant Surgical Procedure

动物心脏移植手术培训

• 批准号: 7215817
• 负责人: michael a solomon
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7215817
• 关键词: heart transplantation; histopathology; laboratory rat; surgery; training; transplantation immunology; veterinary science

This protocol is part of an overall program to study acute cellular rejection, immunotolerance and cardiac allograft vasculopathy in a rat cardiac transplant model. The rodent model of heterotopic cardiac transplantation is a well established model in the field of solid organ transplantation. The purpose of this protocol is to make provisions to train personnel in the rat cardiac transplant surgical procedure. The anticipated outcome is to master the technical skills prior to the initiation of an animal study, thereby minimizing unnecessary animal suffering and loss due to research procedures. The protocol also allows for the harvesting of organs after euthanizing the animal for the purposes of: histopathological review; training individuals in the sample processing techniques; and providing means for experienced laboratory personnel to develop improved sample processing techniques.

该协议是研究大鼠心脏移植模型中急性细胞排斥、免疫耐受和心脏同种异体移植血管病变的总体计划的一部分。啮齿动物异位心脏移植模型是实体器官移植领域成熟的模型。本协议的目的是为大鼠心脏移植手术人员的培训做出规定。预期的结果是在开始动物研究之前掌握技术技能，从而最大限度地减少由于研究程序造成的不必要的动物痛苦和损失。该协议还允许在对动物实施安乐死后采集器官，目的是：组织病理学检查；对个人进行样本处理技术培训；为经验丰富的实验室人员提供改进样品处理技术的手段。