The use of Granulocyte Colony-Stimulating-Factor in Heal

粒细胞集落刺激因子在治疗中的应用

• 批准号: 7331987
• 负责人: David Frank Stroncek
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7331987
• 关键词: use; Granulocyte; Colony; Stimulating; Factor

Granulocyte colony-stimulating factor (G-CSF) is the standard growth factor for mobilizing hematopoietic progenitor cells in healthy peripheral blood progenitor cell (PBPC) donors. G-CSF is given daily for 5 days to PBPC donors prior collecting PBPC concentrates by apheresis. PBPC donors given G-CSF experience splenic enlargement and, rarely, spontaneous rupture of the spleen. We evaluated the incidence and time course of splenic enlargement in PBPC concentrate donors and accessed factors effecting size changes. Healthy adults were given G-CSF (10 micrograms/kg/day) for 5 days and a PBPC concentrate was collected by apheresis. Ultrasound was used to assess craniocaudal spleen length prior to giving G-CSF, the day of apheresis, and 4 or 10 days after apheresis. Among 38 healthy subjects a 5-day course of G-CSF causes spleen length to increase in 95% of the donors. The mean spleen length increased from a baseline length of 10.8 ? 1.7 cm to 12.2 ? 1.7 cm and the mean increase in length was 13%. However, spleen enlargement was transient. Four days after apheresis the spleen length fell to 11.3 ? 1.8 cm, but it remained greater than baseline levels. Ten days after apheresis, spleen length fell to 10.5 ? 1.2 cm and did not differ from baseline length. The magnitude of spleen length increase was not affected by donor gender, race, or age. We are currently investigating the mechanisms of G-CSF mobilization of stem cells into the peripheral blood. Plasma protein profiles are being compared before and after G-CSF administration. Associations between the expression of cell adhesion molecules on mature neutrophils and neutrophil precursors and spleen size change will also be investigated.

粒细胞刺激因子（G-CSF）是动员健康外周血祖细胞（PBPC）供体中造血祖细胞的标准生长因子。 G-CSF每天给PBPC捐赠者进行5天的时间，以前通过放置良心收集PBPC浓缩物。给予G-CSF的PBPC供体经历了脾脏增大，并且很少发生脾脏的自发破裂。我们评估了PBPC浓缩剂供体的脾扩大的发病率和时间过程，并访问了影响大小变化的因素。给予健康的成年人G-CSF（10微克/千克/天），持续5天，并通过格式收集PBPC浓缩液。超声是在给出G-CSF之前，格式（格式）和格式后4或10天之前评估颅脑脾长度。在38名健康受试者中，为期5天的G-CSF导致脾脏长度增加了95％的捐助者。平均脾长度从基线长度增加了10.8？ 1.7厘米至12.2？ 1.7厘米，长度的平均增加为13％。但是，脾脏扩大是短暂的。放逐后四天，脾长度降至11.3？ 1.8厘米，但仍大于基线水平。骨折十天后，脾长度降至10.5？ 1.2厘米，与基线长度没有区别。脾脏长度的增加不受捐助性别，种族或年龄的影响。我们目前正在研究将干细胞动员G-CSF动员到外周血中的机制。在G-CSF给药前后，正在比较血浆蛋白谱。还将研究细胞粘附分子在成熟中性粒细胞和中性粒细胞前体和脾脏大小变化之间的关联。