Structure and Function of Granulocyte Antigens

粒细胞抗原的结构和功能

• 批准号: 7331984
• 负责人: David Frank Stroncek
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7331984
• 关键词: Structure; Function; Granulocyte; Antigens

Granulocyte antigens play an important role in cell function including adhesion, cell activation, and binding of immunoglobulins. The purpose of these studies is to better define the molecular basis of variations in neutrophil antigens and their role in neutrophil function. Neutrophil-specific antigen HNA-2a (NB1) has been localized to NB1 glycoprotein (gp) which is expressed on subpopulations of neutrophils. The gene encoding the NB1 gp was recently sequenced and called NB1. Another group described a gene called PRV-1 that is highly homologous to NB1. We found that NB1 and PRV-1 are alleles of the same gene, CD177, and showed that this gene is located on chromosome 19q13.31. In addition, we found that a pseudo gene homologous to exons 4 through 9 of CD177 was located on adjacent to CD177 on chromosome 19q13.31. The most common polymorphisms of CD177 was found to be a G to C change at bp42. Recently, CD177 has been found to be over expressed in neutrophils from patients with polycythemia vera. On going studies are using the leukemia cell line HL60 to characterize the molecular mechanisms responsible for CD177 expression. Future studies will investigate the molecular basis for the variable expression of CD177 and the expression of CD177 in patients with myeloproliferative diseases.

粒细胞抗原在细胞功能中起重要作用，包括粘附，细胞活化和免疫球蛋白的结合。这些研究的目的是更好地定义中性粒细胞抗原变异的分子基础及其在中性粒细胞功能中的作用。中性粒细胞特异性抗原HNA-2A（NB1）已定位在NB1糖蛋白（GP）上，该糖蛋白（GP）以中性粒细胞的亚群表示。最近对编码NB1 GP的基因进行了测序，称为NB1。另一组描述了一个称为PRV-1的基因，该基因与NB1高度同源。我们发现NB1和PRV-1是同一基因CD177的等位基因，并且表明该基因位于19q13.31染色体上。此外，我们发现CD177的外显子4至9同源的伪基因位于染色体19q13.31上的CD177。发现CD177最常见的多态性是BP42时C变化的A G。最近，已经发现CD177在来自多性细胞增多症的患者的中性粒细胞中过度表达。进行研究的是使用白血病细胞系HL60来表征负责CD177表达的分子机制。未来的研究将研究CD177可变表达和CD177表达的分子基础。